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The Use of Three Long Non-Coding RNAs as Potential Prognostic Indicators of Astrocytoma.

Zhi F, Wang Q, Xue L, Shao N, Wang R, Deng D, Wang S, Xia X, Yang Y - PLoS ONE (2015)

Bottom Line: Long noncoding RNAs (lncRNAs) are pervasively transcribed and play a key role in tumorigenesis.Using Kaplan-Meier survival analysis, we showed that the low expression of BC002811 or XLOC_010967, or the high expression of NR_002809 was significantly associated with poor patient survival.Moreover, Cox proportional hazard regression analysis revealed that this prognostic impact was independent of other clinicopathological factors.

View Article: PubMed Central - PubMed

Affiliation: Modern Medical Research Center, Third Affiliated Hospital of Soochow University, Changzhou, 213000, Jiangsu, China.

ABSTRACT
Long noncoding RNAs (lncRNAs) are pervasively transcribed and play a key role in tumorigenesis. The aim of the study was to determine the lncRNA expression profile in astrocytomas and to assess its potential clinical value. We performed a three-step analysis to establish the lncRNA profile for astrocytoma: a) the lncRNA expression was examined on 3 astrocytomas as well as 3 NATs (normal adjacent tissues) using the lncRNA microarray; b) the top-hits were validated in 40 astrocytomas (WHO grade II-IV) by quantitative real time-PCR (qRT-PCR); c) the hits with significant differences were re-evaluated using qRT-PCR in 90 astrocytomas. Finally, 7 lncRNAs were found to have a significantly different expression profile in astrocytoma samples compared to the NAT samples. Unsupervised clustering analysis further revealed the potential of the 7-lncRNA profile to differentiate between tumors and NAT samples. The upregulation of ENST00000545440 and NR_002809 was associated with advanced clinical stages of astrocytoma. Using Kaplan-Meier survival analysis, we showed that the low expression of BC002811 or XLOC_010967, or the high expression of NR_002809 was significantly associated with poor patient survival. Moreover, Cox proportional hazard regression analysis revealed that this prognostic impact was independent of other clinicopathological factors. Our results indicate that the lncRNA profile may be a potential prognostic biomarker for the prediction of post-surgical outcomes.

No MeSH data available.


Related in: MedlinePlus

The levels of lncRNA expression in the astrocytoma patients stratified by tumor grade.The relative expression levels of ENST00000545440 and NR_002809 in each group are shown.
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pone.0135242.g002: The levels of lncRNA expression in the astrocytoma patients stratified by tumor grade.The relative expression levels of ENST00000545440 and NR_002809 in each group are shown.

Mentions: We subsequently investigated whether lncRNA expression levels represented specific molecular signatures for subsets of astrocytomas. The expression levels of the 7 lncRNAs in the astrocytoma samples were stratified using 3 types of clinicopathological parameters (sex, age, and WHO grade). We assessed the relationship between these clinical features and the lncRNA expression levels. No lncRNAs were found to be differentially expressed when the astrocytoma samples were stratified by age or sex. However, 2 lncRNAs were found to be differentially expressed when the samples were stratified according to tumor grade. As shown in Fig 2, the expression of ENST00000545440 and NR_002809 increased from WHO grade II to WHO grade IV astrocytomas. This result suggests that the upregulation of ENST00000545440 and NR_002809 is associated with advanced clinical stages of astrocytomas.


The Use of Three Long Non-Coding RNAs as Potential Prognostic Indicators of Astrocytoma.

Zhi F, Wang Q, Xue L, Shao N, Wang R, Deng D, Wang S, Xia X, Yang Y - PLoS ONE (2015)

The levels of lncRNA expression in the astrocytoma patients stratified by tumor grade.The relative expression levels of ENST00000545440 and NR_002809 in each group are shown.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4529097&req=5

pone.0135242.g002: The levels of lncRNA expression in the astrocytoma patients stratified by tumor grade.The relative expression levels of ENST00000545440 and NR_002809 in each group are shown.
Mentions: We subsequently investigated whether lncRNA expression levels represented specific molecular signatures for subsets of astrocytomas. The expression levels of the 7 lncRNAs in the astrocytoma samples were stratified using 3 types of clinicopathological parameters (sex, age, and WHO grade). We assessed the relationship between these clinical features and the lncRNA expression levels. No lncRNAs were found to be differentially expressed when the astrocytoma samples were stratified by age or sex. However, 2 lncRNAs were found to be differentially expressed when the samples were stratified according to tumor grade. As shown in Fig 2, the expression of ENST00000545440 and NR_002809 increased from WHO grade II to WHO grade IV astrocytomas. This result suggests that the upregulation of ENST00000545440 and NR_002809 is associated with advanced clinical stages of astrocytomas.

Bottom Line: Long noncoding RNAs (lncRNAs) are pervasively transcribed and play a key role in tumorigenesis.Using Kaplan-Meier survival analysis, we showed that the low expression of BC002811 or XLOC_010967, or the high expression of NR_002809 was significantly associated with poor patient survival.Moreover, Cox proportional hazard regression analysis revealed that this prognostic impact was independent of other clinicopathological factors.

View Article: PubMed Central - PubMed

Affiliation: Modern Medical Research Center, Third Affiliated Hospital of Soochow University, Changzhou, 213000, Jiangsu, China.

ABSTRACT
Long noncoding RNAs (lncRNAs) are pervasively transcribed and play a key role in tumorigenesis. The aim of the study was to determine the lncRNA expression profile in astrocytomas and to assess its potential clinical value. We performed a three-step analysis to establish the lncRNA profile for astrocytoma: a) the lncRNA expression was examined on 3 astrocytomas as well as 3 NATs (normal adjacent tissues) using the lncRNA microarray; b) the top-hits were validated in 40 astrocytomas (WHO grade II-IV) by quantitative real time-PCR (qRT-PCR); c) the hits with significant differences were re-evaluated using qRT-PCR in 90 astrocytomas. Finally, 7 lncRNAs were found to have a significantly different expression profile in astrocytoma samples compared to the NAT samples. Unsupervised clustering analysis further revealed the potential of the 7-lncRNA profile to differentiate between tumors and NAT samples. The upregulation of ENST00000545440 and NR_002809 was associated with advanced clinical stages of astrocytoma. Using Kaplan-Meier survival analysis, we showed that the low expression of BC002811 or XLOC_010967, or the high expression of NR_002809 was significantly associated with poor patient survival. Moreover, Cox proportional hazard regression analysis revealed that this prognostic impact was independent of other clinicopathological factors. Our results indicate that the lncRNA profile may be a potential prognostic biomarker for the prediction of post-surgical outcomes.

No MeSH data available.


Related in: MedlinePlus