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Inhibitory neuron migration and IPL formation in the developing zebrafish retina.

Chow RW, Almeida AD, Randlett O, Norden C, Harris WA - Development (2015)

Bottom Line: All RINs then transition to a less directionally persistent multipolar phase of migration.Finally, HCs, iACs and dACs each undergo cell type-specific migration.In contrast to current hypotheses, we find that most dACs send processes into the forming inner plexiform layer (IPL) before migrating through it and inverting their polarity.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3DY, UK.

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Tangential migration. (A-C) Tangential component of cell tracks of HCs (blue), iACs (green), dACs (magenta) and unclassified inhibitory neurons (grey) for each migration phase. (D-F) Boxplots showing the mean, 95% confidence interval and one standard deviation for magnitude of displacement (D), velocity (E) and the ratio of displacement over total distance travelled (F).
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DEV122473F7: Tangential migration. (A-C) Tangential component of cell tracks of HCs (blue), iACs (green), dACs (magenta) and unclassified inhibitory neurons (grey) for each migration phase. (D-F) Boxplots showing the mean, 95% confidence interval and one standard deviation for magnitude of displacement (D), velocity (E) and the ratio of displacement over total distance travelled (F).

Mentions: Having analysed apico-basal migration, we next investigated the dynamics of tangential migration in the three types of RIN (Fig. 7), as this is crucial for our understanding of how neurons become properly spaced within their respective layers. To begin to answer this question, we analysed the average values for distance and speed for each phase and found that they are not significantly different from one another. However, whereas only 0.05% of RINs migrated >5 µm tangentially, which is roughly one somal length, during Phase 1, 27% of RINs migrated >5 µm in Phase 2 and 22% of RINs migrated >5 µm in the first 12 h of Phase 3, suggesting that tangential migration occurs mainly during later stages of migration. At all phases of migration, cells were seen to migrate tangentially in one direction, only to reverse direction at a later time. This lack of directional persistence often results in low ratios of displacement over distance travelled (Fig. 7F). Thus, it seems that the first bipolar phase of migration is used primarily for getting RINs to the apico-basal centre of the retina, but that the second and third phases of migration are used to refine the position of the cells into specific layers and establish mosaic arrays within these layers.Fig. 7.


Inhibitory neuron migration and IPL formation in the developing zebrafish retina.

Chow RW, Almeida AD, Randlett O, Norden C, Harris WA - Development (2015)

Tangential migration. (A-C) Tangential component of cell tracks of HCs (blue), iACs (green), dACs (magenta) and unclassified inhibitory neurons (grey) for each migration phase. (D-F) Boxplots showing the mean, 95% confidence interval and one standard deviation for magnitude of displacement (D), velocity (E) and the ratio of displacement over total distance travelled (F).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4529032&req=5

DEV122473F7: Tangential migration. (A-C) Tangential component of cell tracks of HCs (blue), iACs (green), dACs (magenta) and unclassified inhibitory neurons (grey) for each migration phase. (D-F) Boxplots showing the mean, 95% confidence interval and one standard deviation for magnitude of displacement (D), velocity (E) and the ratio of displacement over total distance travelled (F).
Mentions: Having analysed apico-basal migration, we next investigated the dynamics of tangential migration in the three types of RIN (Fig. 7), as this is crucial for our understanding of how neurons become properly spaced within their respective layers. To begin to answer this question, we analysed the average values for distance and speed for each phase and found that they are not significantly different from one another. However, whereas only 0.05% of RINs migrated >5 µm tangentially, which is roughly one somal length, during Phase 1, 27% of RINs migrated >5 µm in Phase 2 and 22% of RINs migrated >5 µm in the first 12 h of Phase 3, suggesting that tangential migration occurs mainly during later stages of migration. At all phases of migration, cells were seen to migrate tangentially in one direction, only to reverse direction at a later time. This lack of directional persistence often results in low ratios of displacement over distance travelled (Fig. 7F). Thus, it seems that the first bipolar phase of migration is used primarily for getting RINs to the apico-basal centre of the retina, but that the second and third phases of migration are used to refine the position of the cells into specific layers and establish mosaic arrays within these layers.Fig. 7.

Bottom Line: All RINs then transition to a less directionally persistent multipolar phase of migration.Finally, HCs, iACs and dACs each undergo cell type-specific migration.In contrast to current hypotheses, we find that most dACs send processes into the forming inner plexiform layer (IPL) before migrating through it and inverting their polarity.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3DY, UK.

Show MeSH