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Inhibitory neuron migration and IPL formation in the developing zebrafish retina.

Chow RW, Almeida AD, Randlett O, Norden C, Harris WA - Development (2015)

Bottom Line: Finally, HCs, iACs and dACs each undergo cell type-specific migration.In contrast to current hypotheses, we find that most dACs send processes into the forming inner plexiform layer (IPL) before migrating through it and inverting their polarity.By imaging and quantifying the dynamics of HCs, iACs and dACs from birth to final position, this study thus provides evidence for distinct and new migration patterns during retinal lamination and insights into the initiation of IPL formation.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3DY, UK.

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iACs and dACs undergo a stereotypical sequence of behaviours to polarize and migrate to their respective cell layers. (A) Example of an iAC (blue arrowhead) stratifying in the INL. (B,C) Two examples of dACs (white) migrating into the GCL. Times are shown in h:min relative to the start of the movies, ∼48 hpf.
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DEV122473F4: iACs and dACs undergo a stereotypical sequence of behaviours to polarize and migrate to their respective cell layers. (A) Example of an iAC (blue arrowhead) stratifying in the INL. (B,C) Two examples of dACs (white) migrating into the GCL. Times are shown in h:min relative to the start of the movies, ∼48 hpf.

Mentions: We next turned our attention to iACs. We followed 50 iACs throughout the multipolar phase (Fig. 4A; supplementary material Movie 4). Consistent with a previous zebrafish study (Godinho et al., 2005), multipolar iACs appear to stabilize processes that are in contact with the forming IPL (n=50 cells; Fig. 4A; supplementary material Movies 4 and 5). During stratification, some of these cells were seen to extend short, tangentially directed protrusions and shuffle their somas tangentially (supplementary material Fig. S4A). Out of the 29 iACs we followed with somas situated one or more cell body diameters apical of the IPL at the end of the imaging session, all were seen at least once during the time-lapse period to be directly adjacent to the IPL, only to move away from the IPL later in the movie (Fig. 4A). Thus, it is not the case that iACs simply pile up over the IPL according to their time of arrival as suggested previously (Rapaport, 2006; Voinescu et al., 2009).Fig. 4.


Inhibitory neuron migration and IPL formation in the developing zebrafish retina.

Chow RW, Almeida AD, Randlett O, Norden C, Harris WA - Development (2015)

iACs and dACs undergo a stereotypical sequence of behaviours to polarize and migrate to their respective cell layers. (A) Example of an iAC (blue arrowhead) stratifying in the INL. (B,C) Two examples of dACs (white) migrating into the GCL. Times are shown in h:min relative to the start of the movies, ∼48 hpf.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4529032&req=5

DEV122473F4: iACs and dACs undergo a stereotypical sequence of behaviours to polarize and migrate to their respective cell layers. (A) Example of an iAC (blue arrowhead) stratifying in the INL. (B,C) Two examples of dACs (white) migrating into the GCL. Times are shown in h:min relative to the start of the movies, ∼48 hpf.
Mentions: We next turned our attention to iACs. We followed 50 iACs throughout the multipolar phase (Fig. 4A; supplementary material Movie 4). Consistent with a previous zebrafish study (Godinho et al., 2005), multipolar iACs appear to stabilize processes that are in contact with the forming IPL (n=50 cells; Fig. 4A; supplementary material Movies 4 and 5). During stratification, some of these cells were seen to extend short, tangentially directed protrusions and shuffle their somas tangentially (supplementary material Fig. S4A). Out of the 29 iACs we followed with somas situated one or more cell body diameters apical of the IPL at the end of the imaging session, all were seen at least once during the time-lapse period to be directly adjacent to the IPL, only to move away from the IPL later in the movie (Fig. 4A). Thus, it is not the case that iACs simply pile up over the IPL according to their time of arrival as suggested previously (Rapaport, 2006; Voinescu et al., 2009).Fig. 4.

Bottom Line: Finally, HCs, iACs and dACs each undergo cell type-specific migration.In contrast to current hypotheses, we find that most dACs send processes into the forming inner plexiform layer (IPL) before migrating through it and inverting their polarity.By imaging and quantifying the dynamics of HCs, iACs and dACs from birth to final position, this study thus provides evidence for distinct and new migration patterns during retinal lamination and insights into the initiation of IPL formation.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3DY, UK.

Show MeSH