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Chemical composition, antinociceptive, anti-inflammatory and redox properties in vitro of the essential oil from Remirea maritima Aubl. (Cyperaceae).

Rabelo AS, Serafini MR, Rabelo TK, de Melo MG, da Silva Prado D, Gelain DP, Moreira JC, dos Santos Bezerra M, da Silva TB, Costa EV, de Lima Nogueira PC, de Souza Moraes VR, do Nascimento Prata AP, Quintans LJ, Araújo AA - BMC Complement Altern Med (2014)

Bottom Line: NO radical generated at physiological pH was found to be inhibited by RMO, that showed scavenging effect upon SNP-induced NO production at all concentrations.On the other hand RMO did not present any scavenging effect in H2O2 formation in vitro.The results of this study revealed that RMO has both peripheral and central analgesic properties.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Ensaios Farmacêuticos e Toxicidade, Universidade Federal de Sergipe (LeFT/UFS), 49100-000, São Cristóvão, Sergipe, Brazil. alessandrarabelo@hotmail.com.

ABSTRACT

Background: The present study was carried out to evaluate antioxidant, antinociceptive and anti-inflammatory activities of essential oil from R. maritima (RMO) in experimental protocols.

Methods: The essential oil from the roots and rhizomes of RMO were obtained by hydrodistillation using a Clevenger apparatus, and analyzed by gas chromatography/mass spectrometry (GC/MS). Here, we evaluated free radical scavenging activities and antioxidant potential of RMO using in vitro assays for scavenging activity against hydroxyl radicals, hydrogen peroxide, superoxide radicals, and nitric oxide. The total reactive antioxidant potential (TRAP) and total antioxidant reactivity (TAR) indexes and in vitro lipoperoxidation were also evaluated. The ability of RMO to prevent lipid peroxidation was measured by quantifying thiobarbituric acid-reactive substances (TBARS). NO radical generated at physiological pH was found to be inhibited by RMO, that showed scavenging effect upon SNP-induced NO production at all concentrations. Antinociceptive and anti-inflammatory properties were evaluated by acetic acid writhing reflex, Formalin-induced nociception and Carrageenan-induced edema test.

Results: The majors compounds identified was remirol (43.2%), cyperene (13.8%), iso-evodionol (5.8%), cyperotundone (5.7%), caryophyllene oxide (4.9%), and rotundene (4.6%). At the TRAP assay, RMO concentration of 1 mg.mL(-1) showed anti-oxidant effects and at concentration of 1 and 10 ng.mL(-1) RMO showed pro-oxidant effect. RMO at 1 mg.mL(-1) also showed significant anti-oxidant capacity in TAR measurement. Concentrations of RMO from 1 ng.mL(-1) to 100 μg.mL(-1) enhanced the AAPH-induced lipoperoxidation. RMO reduced deoxyribose oxidative damage, induced by the Fenton reaction induction system, at concentrations from 1 ng.mL(-1) to 100 μg.mL(-1). We observed that RMO caused a significant increase in rate of adrenaline auto-oxidation. On the other hand RMO did not present any scavenging effect in H2O2 formation in vitro. The results of this study revealed that RMO has both peripheral and central analgesic properties. The RMO, all doses, orally (p.o.) administered significantly inhibited (p < 0.05, p < 0.01 and p < 0.001) the acetic acid-induced writhings and two phases of formalin-induced nociception in mice.

Conclusion: The RMO demonstrated antioxidant and analgesic profile which may be related to the composition of the oil.

No MeSH data available.


Related in: MedlinePlus

Thiobarbituric acid-reactive substances (TBARS)in vitro. A lipid-rich system was incubated with a free radical source (AAPH) and the effect of different concentrations of RMO on the lipoperoxidation was measured by quantifying TBARS. Control is incubation medium without AAPH; other groups contained AAPH alone or in the presence of different concentrations of RMO or its vehicle alone. Bars represent mean ± SEM. *p < 0.05, **p < 0.0001 (1-way ANOVA followed by Tukey’s multiple comparison post test).
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Fig3: Thiobarbituric acid-reactive substances (TBARS)in vitro. A lipid-rich system was incubated with a free radical source (AAPH) and the effect of different concentrations of RMO on the lipoperoxidation was measured by quantifying TBARS. Control is incubation medium without AAPH; other groups contained AAPH alone or in the presence of different concentrations of RMO or its vehicle alone. Bars represent mean ± SEM. *p < 0.05, **p < 0.0001 (1-way ANOVA followed by Tukey’s multiple comparison post test).

Mentions: Lipid peroxidation has been defined as the biological damage caused by free radicals that are formed under oxidative stress [30]. The ability of RMO to prevent lipid peroxidation was measured by quantifying thiobarbituric acid-reactive substances (TBARS) generated by AAPH in a lipid-rich incubation medium. The effect of different concentrations on lipid peroxidation is shown in Figure 3. Concentrations of RMO from 1 ng.mL−1 to 100 μg.mL−1 enhanced the AAPH-induced lipoperoxidation.In this study, the ROM was checked for its inhibitory effect on nitric oxide production by the method of Griess. NO radical generated from sodium nitroprusside at physiological pH was found to be inhibited by ROM, that showed scavenging effect upon SNP-induced NO production at all concentrations (Figure 4).Figure 3


Chemical composition, antinociceptive, anti-inflammatory and redox properties in vitro of the essential oil from Remirea maritima Aubl. (Cyperaceae).

Rabelo AS, Serafini MR, Rabelo TK, de Melo MG, da Silva Prado D, Gelain DP, Moreira JC, dos Santos Bezerra M, da Silva TB, Costa EV, de Lima Nogueira PC, de Souza Moraes VR, do Nascimento Prata AP, Quintans LJ, Araújo AA - BMC Complement Altern Med (2014)

Thiobarbituric acid-reactive substances (TBARS)in vitro. A lipid-rich system was incubated with a free radical source (AAPH) and the effect of different concentrations of RMO on the lipoperoxidation was measured by quantifying TBARS. Control is incubation medium without AAPH; other groups contained AAPH alone or in the presence of different concentrations of RMO or its vehicle alone. Bars represent mean ± SEM. *p < 0.05, **p < 0.0001 (1-way ANOVA followed by Tukey’s multiple comparison post test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4528852&req=5

Fig3: Thiobarbituric acid-reactive substances (TBARS)in vitro. A lipid-rich system was incubated with a free radical source (AAPH) and the effect of different concentrations of RMO on the lipoperoxidation was measured by quantifying TBARS. Control is incubation medium without AAPH; other groups contained AAPH alone or in the presence of different concentrations of RMO or its vehicle alone. Bars represent mean ± SEM. *p < 0.05, **p < 0.0001 (1-way ANOVA followed by Tukey’s multiple comparison post test).
Mentions: Lipid peroxidation has been defined as the biological damage caused by free radicals that are formed under oxidative stress [30]. The ability of RMO to prevent lipid peroxidation was measured by quantifying thiobarbituric acid-reactive substances (TBARS) generated by AAPH in a lipid-rich incubation medium. The effect of different concentrations on lipid peroxidation is shown in Figure 3. Concentrations of RMO from 1 ng.mL−1 to 100 μg.mL−1 enhanced the AAPH-induced lipoperoxidation.In this study, the ROM was checked for its inhibitory effect on nitric oxide production by the method of Griess. NO radical generated from sodium nitroprusside at physiological pH was found to be inhibited by ROM, that showed scavenging effect upon SNP-induced NO production at all concentrations (Figure 4).Figure 3

Bottom Line: NO radical generated at physiological pH was found to be inhibited by RMO, that showed scavenging effect upon SNP-induced NO production at all concentrations.On the other hand RMO did not present any scavenging effect in H2O2 formation in vitro.The results of this study revealed that RMO has both peripheral and central analgesic properties.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Ensaios Farmacêuticos e Toxicidade, Universidade Federal de Sergipe (LeFT/UFS), 49100-000, São Cristóvão, Sergipe, Brazil. alessandrarabelo@hotmail.com.

ABSTRACT

Background: The present study was carried out to evaluate antioxidant, antinociceptive and anti-inflammatory activities of essential oil from R. maritima (RMO) in experimental protocols.

Methods: The essential oil from the roots and rhizomes of RMO were obtained by hydrodistillation using a Clevenger apparatus, and analyzed by gas chromatography/mass spectrometry (GC/MS). Here, we evaluated free radical scavenging activities and antioxidant potential of RMO using in vitro assays for scavenging activity against hydroxyl radicals, hydrogen peroxide, superoxide radicals, and nitric oxide. The total reactive antioxidant potential (TRAP) and total antioxidant reactivity (TAR) indexes and in vitro lipoperoxidation were also evaluated. The ability of RMO to prevent lipid peroxidation was measured by quantifying thiobarbituric acid-reactive substances (TBARS). NO radical generated at physiological pH was found to be inhibited by RMO, that showed scavenging effect upon SNP-induced NO production at all concentrations. Antinociceptive and anti-inflammatory properties were evaluated by acetic acid writhing reflex, Formalin-induced nociception and Carrageenan-induced edema test.

Results: The majors compounds identified was remirol (43.2%), cyperene (13.8%), iso-evodionol (5.8%), cyperotundone (5.7%), caryophyllene oxide (4.9%), and rotundene (4.6%). At the TRAP assay, RMO concentration of 1 mg.mL(-1) showed anti-oxidant effects and at concentration of 1 and 10 ng.mL(-1) RMO showed pro-oxidant effect. RMO at 1 mg.mL(-1) also showed significant anti-oxidant capacity in TAR measurement. Concentrations of RMO from 1 ng.mL(-1) to 100 μg.mL(-1) enhanced the AAPH-induced lipoperoxidation. RMO reduced deoxyribose oxidative damage, induced by the Fenton reaction induction system, at concentrations from 1 ng.mL(-1) to 100 μg.mL(-1). We observed that RMO caused a significant increase in rate of adrenaline auto-oxidation. On the other hand RMO did not present any scavenging effect in H2O2 formation in vitro. The results of this study revealed that RMO has both peripheral and central analgesic properties. The RMO, all doses, orally (p.o.) administered significantly inhibited (p < 0.05, p < 0.01 and p < 0.001) the acetic acid-induced writhings and two phases of formalin-induced nociception in mice.

Conclusion: The RMO demonstrated antioxidant and analgesic profile which may be related to the composition of the oil.

No MeSH data available.


Related in: MedlinePlus