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Evaluation of mesenchymal stem cells in treatment of infertility in male rats.

Hassan AI, Alam SS - Stem Cell Res Ther (2014)

Bottom Line: Lead nitrate caused degeneration, necrosis, interstitial edema, and reduction in spermatogenic activity in some seminiferous tubules.It was concluded that lead is a gonadotoxic with a tendency of suppressing semen characteristics and testosterone levels of animals, the presence of MSCs was found to alleviate the toxic effects of lead.We conclude that MSCs derived from the bone marrow of rats can be an effective therapy of LN induced gonado toxicity, thus can contribute to the treatment of infertility.

View Article: PubMed Central - PubMed

ABSTRACT

Introduction: The present study aimed to elucidate the therapeutic effects of mesenchymal stem cells (MSCs) derived from the bone marrow of rats (BM) against toxic effects of lead (Pb) on the male gonads of experimental rats.

Methods: The experimental animals were exposed to lead in the form of lead nitrate (LN) one quarter of the LD50. The efficacy of MSCs to reduce gonado-totoxicity induced by lead nitrate at 21, 30 and 60 days, was evaluated experimentally in male rats.

Results: The results showed that testosterone levels and semen quality ameliorated following treatment with MSCs. Also, superoxide dismutase, glutathione peroxidase and catalase levels were increased 21, 30 and 60 days post treatment of MSCs. Moreover, a decrease in genomic DNA alteration and percentage of fragmented DNA was recorded after MSCs treatment. Lead nitrate caused degeneration, necrosis, interstitial edema, and reduction in spermatogenic activity in some seminiferous tubules. The LN-induced changes in histopathologic findings of testis were partially reversed by treatment with MSCs. Histological examination of testis showed deformities in morphology of testis in test animals with gross damage within the seminiferous tubules in Lead nitrate group. The LN-induced changes in histopathologic findings of testis were partially reversed by treatment of MSCs.

Conclusions: It was concluded that lead is a gonadotoxic with a tendency of suppressing semen characteristics and testosterone levels of animals, the presence of MSCs was found to alleviate the toxic effects of lead. We conclude that MSCs derived from the bone marrow of rats can be an effective therapy of LN induced gonado toxicity, thus can contribute to the treatment of infertility.

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Related in: MedlinePlus

RAPD-PCR fingerprinting of sperm generated by primers OPA01 (A), OPA08 (B), OPA12 (C) and OPA20 (D). The appearance and disappearance of bands of the same pattern as the negative control were considered. Rat samples treated with lead nitrate after 21 days (Lane 7), 30 days (Lane 5) and 60 days (Lane 3). Rat samples treated with both lead nitrate and MSCs/BM after 21 days (Lane 6), 30 days (Lane 4) and 60 days (Lane 2). Normal control group (Lane 1). M = marker 1,000 bp. MSCs/BM, bone marrow-derived mesenchymal stem cells; RAPD, random amplified polymorphic DNA.
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Fig5: RAPD-PCR fingerprinting of sperm generated by primers OPA01 (A), OPA08 (B), OPA12 (C) and OPA20 (D). The appearance and disappearance of bands of the same pattern as the negative control were considered. Rat samples treated with lead nitrate after 21 days (Lane 7), 30 days (Lane 5) and 60 days (Lane 3). Rat samples treated with both lead nitrate and MSCs/BM after 21 days (Lane 6), 30 days (Lane 4) and 60 days (Lane 2). Normal control group (Lane 1). M = marker 1,000 bp. MSCs/BM, bone marrow-derived mesenchymal stem cells; RAPD, random amplified polymorphic DNA.

Mentions: The total number of amplification products generated by these individual primers and variable fragments are described in Table 1, and representative RAPD fingerprints are shown in Figures 5A-D.Alterations in RAPD ‘fingerprints’ produced by the random primer set were seen in one of two ways. There was the gain or loss of a band. The quantitative analysis of those bands, expressed as percentage of band loss and gain, shows a time-dependent relationship. In the case of band gain, at 21 days after lead treatment 16 new bands were amplified, representing 36.3%, while at 30 and 60 days after lead treatment, 14 and 9 new bands were amplified, representing 31.8% and 20.4%, respectively. The trend of increase in band gain as related to the decrease in time after lead exposure is depicted in Figure 6A.Similarly, in the case of band loss, at 21 days after lead treatment eight bands had disappeared representing 18.2% (Figure 6B). At 30 and 60 days, nine and six bands had disappeared representing 20.4% and 13.6%, respectively.Treatment with stem cells ameliorated the effects of lead at different time intervals as evidenced by a decrease in the percentage of polymorphic bands (loss and gains) as shown in (Figures 6A and B).Figure 5


Evaluation of mesenchymal stem cells in treatment of infertility in male rats.

Hassan AI, Alam SS - Stem Cell Res Ther (2014)

RAPD-PCR fingerprinting of sperm generated by primers OPA01 (A), OPA08 (B), OPA12 (C) and OPA20 (D). The appearance and disappearance of bands of the same pattern as the negative control were considered. Rat samples treated with lead nitrate after 21 days (Lane 7), 30 days (Lane 5) and 60 days (Lane 3). Rat samples treated with both lead nitrate and MSCs/BM after 21 days (Lane 6), 30 days (Lane 4) and 60 days (Lane 2). Normal control group (Lane 1). M = marker 1,000 bp. MSCs/BM, bone marrow-derived mesenchymal stem cells; RAPD, random amplified polymorphic DNA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4528845&req=5

Fig5: RAPD-PCR fingerprinting of sperm generated by primers OPA01 (A), OPA08 (B), OPA12 (C) and OPA20 (D). The appearance and disappearance of bands of the same pattern as the negative control were considered. Rat samples treated with lead nitrate after 21 days (Lane 7), 30 days (Lane 5) and 60 days (Lane 3). Rat samples treated with both lead nitrate and MSCs/BM after 21 days (Lane 6), 30 days (Lane 4) and 60 days (Lane 2). Normal control group (Lane 1). M = marker 1,000 bp. MSCs/BM, bone marrow-derived mesenchymal stem cells; RAPD, random amplified polymorphic DNA.
Mentions: The total number of amplification products generated by these individual primers and variable fragments are described in Table 1, and representative RAPD fingerprints are shown in Figures 5A-D.Alterations in RAPD ‘fingerprints’ produced by the random primer set were seen in one of two ways. There was the gain or loss of a band. The quantitative analysis of those bands, expressed as percentage of band loss and gain, shows a time-dependent relationship. In the case of band gain, at 21 days after lead treatment 16 new bands were amplified, representing 36.3%, while at 30 and 60 days after lead treatment, 14 and 9 new bands were amplified, representing 31.8% and 20.4%, respectively. The trend of increase in band gain as related to the decrease in time after lead exposure is depicted in Figure 6A.Similarly, in the case of band loss, at 21 days after lead treatment eight bands had disappeared representing 18.2% (Figure 6B). At 30 and 60 days, nine and six bands had disappeared representing 20.4% and 13.6%, respectively.Treatment with stem cells ameliorated the effects of lead at different time intervals as evidenced by a decrease in the percentage of polymorphic bands (loss and gains) as shown in (Figures 6A and B).Figure 5

Bottom Line: Lead nitrate caused degeneration, necrosis, interstitial edema, and reduction in spermatogenic activity in some seminiferous tubules.It was concluded that lead is a gonadotoxic with a tendency of suppressing semen characteristics and testosterone levels of animals, the presence of MSCs was found to alleviate the toxic effects of lead.We conclude that MSCs derived from the bone marrow of rats can be an effective therapy of LN induced gonado toxicity, thus can contribute to the treatment of infertility.

View Article: PubMed Central - PubMed

ABSTRACT

Introduction: The present study aimed to elucidate the therapeutic effects of mesenchymal stem cells (MSCs) derived from the bone marrow of rats (BM) against toxic effects of lead (Pb) on the male gonads of experimental rats.

Methods: The experimental animals were exposed to lead in the form of lead nitrate (LN) one quarter of the LD50. The efficacy of MSCs to reduce gonado-totoxicity induced by lead nitrate at 21, 30 and 60 days, was evaluated experimentally in male rats.

Results: The results showed that testosterone levels and semen quality ameliorated following treatment with MSCs. Also, superoxide dismutase, glutathione peroxidase and catalase levels were increased 21, 30 and 60 days post treatment of MSCs. Moreover, a decrease in genomic DNA alteration and percentage of fragmented DNA was recorded after MSCs treatment. Lead nitrate caused degeneration, necrosis, interstitial edema, and reduction in spermatogenic activity in some seminiferous tubules. The LN-induced changes in histopathologic findings of testis were partially reversed by treatment with MSCs. Histological examination of testis showed deformities in morphology of testis in test animals with gross damage within the seminiferous tubules in Lead nitrate group. The LN-induced changes in histopathologic findings of testis were partially reversed by treatment of MSCs.

Conclusions: It was concluded that lead is a gonadotoxic with a tendency of suppressing semen characteristics and testosterone levels of animals, the presence of MSCs was found to alleviate the toxic effects of lead. We conclude that MSCs derived from the bone marrow of rats can be an effective therapy of LN induced gonado toxicity, thus can contribute to the treatment of infertility.

Show MeSH
Related in: MedlinePlus