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Light, heat, action: neural control of fruit fly behaviour.

Owald D, Lin S, Waddell S - Philos. Trans. R. Soc. Lond., B, Biol. Sci. (2015)

Bottom Line: The fruit fly Drosophila melanogaster has emerged as a popular model to investigate fundamental principles of neural circuit operation.Relatively recent genetic and technical advances provide the means to specifically and reproducibly manipulate the function of many fly neurons with temporal resolution.In this review, we discuss these approaches with particular emphasis on the pioneering studies and those involving learning and memory.

View Article: PubMed Central - PubMed

Affiliation: Centre for Neural Circuits and Behaviour, University of Oxford, Tinsley Building, Mansfield Road, Oxford OX1 3SR, UK.

ABSTRACT
The fruit fly Drosophila melanogaster has emerged as a popular model to investigate fundamental principles of neural circuit operation. The sophisticated genetics and small brain permit a cellular resolution understanding of innate and learned behavioural processes. Relatively recent genetic and technical advances provide the means to specifically and reproducibly manipulate the function of many fly neurons with temporal resolution. The same cellular precision can also be exploited to express genetically encoded reporters of neural activity and cell-signalling pathways. Combining these approaches in living behaving animals has great potential to generate a holistic view of behavioural control that transcends the usual molecular, cellular and systems boundaries. In this review, we discuss these approaches with particular emphasis on the pioneering studies and those involving learning and memory.

No MeSH data available.


Related in: MedlinePlus

Schematic of a synapse illustrating the main effector and reporter transgenes discussed in the review. The temperature-controlled TRPM8 and dTrpA1, the light-activated CsChrimson and ReaChR (not shown) and the ATP receptor P2X2 gate cation influx. The Shibirets1 encoded temperature-sensitive dynamin (yellow) is a critical part of the synaptic vesicle exo/endocytosis machinery. The oligomeric nature of dynamin presumably accounts for Shibirets1 dominant negativity. Synapto-pHluorin is localized to the synaptic vesicle lumen and its fluorescence increases as the acidified vesicle docks with the plasma membrane and releases the vesicle contents. GCaMP and ArcLight can report neural activation in both the pre- and post-synaptic compartment.
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RSTB20140211F4: Schematic of a synapse illustrating the main effector and reporter transgenes discussed in the review. The temperature-controlled TRPM8 and dTrpA1, the light-activated CsChrimson and ReaChR (not shown) and the ATP receptor P2X2 gate cation influx. The Shibirets1 encoded temperature-sensitive dynamin (yellow) is a critical part of the synaptic vesicle exo/endocytosis machinery. The oligomeric nature of dynamin presumably accounts for Shibirets1 dominant negativity. Synapto-pHluorin is localized to the synaptic vesicle lumen and its fluorescence increases as the acidified vesicle docks with the plasma membrane and releases the vesicle contents. GCaMP and ArcLight can report neural activation in both the pre- and post-synaptic compartment.

Mentions: The most successful tools that have been developed can be driven by the GAL4-, LexA- or QF-based systems and provide the ability to control specific neurons with temporal resolution (figure 3). In this section, we will overview the various effectors (figure 4)—putting their development in a historical context. However, the reader should note that beyond discussing the founding examples, other studies have been selected to highlight a particular use, and it is not our objective to provide a comprehensive list here.Figure 3.


Light, heat, action: neural control of fruit fly behaviour.

Owald D, Lin S, Waddell S - Philos. Trans. R. Soc. Lond., B, Biol. Sci. (2015)

Schematic of a synapse illustrating the main effector and reporter transgenes discussed in the review. The temperature-controlled TRPM8 and dTrpA1, the light-activated CsChrimson and ReaChR (not shown) and the ATP receptor P2X2 gate cation influx. The Shibirets1 encoded temperature-sensitive dynamin (yellow) is a critical part of the synaptic vesicle exo/endocytosis machinery. The oligomeric nature of dynamin presumably accounts for Shibirets1 dominant negativity. Synapto-pHluorin is localized to the synaptic vesicle lumen and its fluorescence increases as the acidified vesicle docks with the plasma membrane and releases the vesicle contents. GCaMP and ArcLight can report neural activation in both the pre- and post-synaptic compartment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4528823&req=5

RSTB20140211F4: Schematic of a synapse illustrating the main effector and reporter transgenes discussed in the review. The temperature-controlled TRPM8 and dTrpA1, the light-activated CsChrimson and ReaChR (not shown) and the ATP receptor P2X2 gate cation influx. The Shibirets1 encoded temperature-sensitive dynamin (yellow) is a critical part of the synaptic vesicle exo/endocytosis machinery. The oligomeric nature of dynamin presumably accounts for Shibirets1 dominant negativity. Synapto-pHluorin is localized to the synaptic vesicle lumen and its fluorescence increases as the acidified vesicle docks with the plasma membrane and releases the vesicle contents. GCaMP and ArcLight can report neural activation in both the pre- and post-synaptic compartment.
Mentions: The most successful tools that have been developed can be driven by the GAL4-, LexA- or QF-based systems and provide the ability to control specific neurons with temporal resolution (figure 3). In this section, we will overview the various effectors (figure 4)—putting their development in a historical context. However, the reader should note that beyond discussing the founding examples, other studies have been selected to highlight a particular use, and it is not our objective to provide a comprehensive list here.Figure 3.

Bottom Line: The fruit fly Drosophila melanogaster has emerged as a popular model to investigate fundamental principles of neural circuit operation.Relatively recent genetic and technical advances provide the means to specifically and reproducibly manipulate the function of many fly neurons with temporal resolution.In this review, we discuss these approaches with particular emphasis on the pioneering studies and those involving learning and memory.

View Article: PubMed Central - PubMed

Affiliation: Centre for Neural Circuits and Behaviour, University of Oxford, Tinsley Building, Mansfield Road, Oxford OX1 3SR, UK.

ABSTRACT
The fruit fly Drosophila melanogaster has emerged as a popular model to investigate fundamental principles of neural circuit operation. The sophisticated genetics and small brain permit a cellular resolution understanding of innate and learned behavioural processes. Relatively recent genetic and technical advances provide the means to specifically and reproducibly manipulate the function of many fly neurons with temporal resolution. The same cellular precision can also be exploited to express genetically encoded reporters of neural activity and cell-signalling pathways. Combining these approaches in living behaving animals has great potential to generate a holistic view of behavioural control that transcends the usual molecular, cellular and systems boundaries. In this review, we discuss these approaches with particular emphasis on the pioneering studies and those involving learning and memory.

No MeSH data available.


Related in: MedlinePlus