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Perspective in infertility: the ovarian stem cells.

Silvestris E, D'Oronzo S, Cafforio P, D'Amato G, Loverro G - J Ovarian Res (2015)

Bottom Line: A considerable progress has been recently pursued in the field of the reproductive medicine and the infertility treatment may account for novel and modern procedures such as in vitro oocyte fertilization, egg donation, pregnancy surrogacy and preimplantation diagnosis.However, great interest has lately been reserved to the ovarian stem cells (OSCs) whose existence in woman ovaries has been proven.OSCs are thus suitable for developmental studies in infertility and in other clinical applications as endocrine derangements due to premature ovarian failure, or for infertility treatment after cancer chemotherapies, as well as in restoring the hormonal balance in postmenopausal age.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Sciences and Human Oncology, Division of Gynecology and Obstetrics, University of Bari 'Aldo Moro', P.za Giulio Cesare, 11, Bari, 70124, Italy. ericasilvestris85@gmail.com.

ABSTRACT
Infertility is a medical and social condition that affects millions of women worldwide and is today considered so far as a new disease. A considerable progress has been recently pursued in the field of the reproductive medicine and the infertility treatment may account for novel and modern procedures such as in vitro oocyte fertilization, egg donation, pregnancy surrogacy and preimplantation diagnosis. However, great interest has lately been reserved to the ovarian stem cells (OSCs) whose existence in woman ovaries has been proven. OSCs are thus suitable for developmental studies in infertility and in other clinical applications as endocrine derangements due to premature ovarian failure, or for infertility treatment after cancer chemotherapies, as well as in restoring the hormonal balance in postmenopausal age.

No MeSH data available.


Related in: MedlinePlus

Ddx4 localization on immunomagnetic isolated OSC. Confocal microscopy showing a single OSC of small size with large nuclei counterstained by DAPI (C) and few cytoplasm. Ddx4, revealed by FITC (green) in B, localizes in vicinity of the membrane actin (red in A). By merging both FITC and PE, Ddx4 is localized outside of the nucleus and shows high fluorescence intensity. Other nuclei stained by DAPI belong to Ddx4-negative cells within the preparation
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Fig4: Ddx4 localization on immunomagnetic isolated OSC. Confocal microscopy showing a single OSC of small size with large nuclei counterstained by DAPI (C) and few cytoplasm. Ddx4, revealed by FITC (green) in B, localizes in vicinity of the membrane actin (red in A). By merging both FITC and PE, Ddx4 is localized outside of the nucleus and shows high fluorescence intensity. Other nuclei stained by DAPI belong to Ddx4-negative cells within the preparation

Mentions: To verify the enrichment in OSCs of the cell suspension obtained by immunomagnetic selection, we evaluated their phenotype by flow cytometry using both FITC-conjugated anti-Ddx-4 and PE-conjugated anti-human OCT4A as ovary lineage specific marker. The flow cytometry analysis is illustrated in Fig. 3. The Ddx4+ cell population was increased up to 24 % (lower section: right) of the original 2 % (left) value as detected in the initial cortical ovarian suspension, with a remarkable fluorescence intensity suggesting the high expression of Ddx4 molecule. Ddx4 was also detected by confocal microscopy revealing high Ddx4 fluorescence intensity that supported the flow cytometry data (Fig. 4). Therefore, the evidence of Ddx4 on OCT4A+ cells from ovarian cortex by both flow cytometry and fluorescence microscopy provided further evidence to the existence of OSCs in the human ovary and that their sorting is reliable by appropriate combined separation methodology.Fig. 3


Perspective in infertility: the ovarian stem cells.

Silvestris E, D'Oronzo S, Cafforio P, D'Amato G, Loverro G - J Ovarian Res (2015)

Ddx4 localization on immunomagnetic isolated OSC. Confocal microscopy showing a single OSC of small size with large nuclei counterstained by DAPI (C) and few cytoplasm. Ddx4, revealed by FITC (green) in B, localizes in vicinity of the membrane actin (red in A). By merging both FITC and PE, Ddx4 is localized outside of the nucleus and shows high fluorescence intensity. Other nuclei stained by DAPI belong to Ddx4-negative cells within the preparation
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4528806&req=5

Fig4: Ddx4 localization on immunomagnetic isolated OSC. Confocal microscopy showing a single OSC of small size with large nuclei counterstained by DAPI (C) and few cytoplasm. Ddx4, revealed by FITC (green) in B, localizes in vicinity of the membrane actin (red in A). By merging both FITC and PE, Ddx4 is localized outside of the nucleus and shows high fluorescence intensity. Other nuclei stained by DAPI belong to Ddx4-negative cells within the preparation
Mentions: To verify the enrichment in OSCs of the cell suspension obtained by immunomagnetic selection, we evaluated their phenotype by flow cytometry using both FITC-conjugated anti-Ddx-4 and PE-conjugated anti-human OCT4A as ovary lineage specific marker. The flow cytometry analysis is illustrated in Fig. 3. The Ddx4+ cell population was increased up to 24 % (lower section: right) of the original 2 % (left) value as detected in the initial cortical ovarian suspension, with a remarkable fluorescence intensity suggesting the high expression of Ddx4 molecule. Ddx4 was also detected by confocal microscopy revealing high Ddx4 fluorescence intensity that supported the flow cytometry data (Fig. 4). Therefore, the evidence of Ddx4 on OCT4A+ cells from ovarian cortex by both flow cytometry and fluorescence microscopy provided further evidence to the existence of OSCs in the human ovary and that their sorting is reliable by appropriate combined separation methodology.Fig. 3

Bottom Line: A considerable progress has been recently pursued in the field of the reproductive medicine and the infertility treatment may account for novel and modern procedures such as in vitro oocyte fertilization, egg donation, pregnancy surrogacy and preimplantation diagnosis.However, great interest has lately been reserved to the ovarian stem cells (OSCs) whose existence in woman ovaries has been proven.OSCs are thus suitable for developmental studies in infertility and in other clinical applications as endocrine derangements due to premature ovarian failure, or for infertility treatment after cancer chemotherapies, as well as in restoring the hormonal balance in postmenopausal age.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Sciences and Human Oncology, Division of Gynecology and Obstetrics, University of Bari 'Aldo Moro', P.za Giulio Cesare, 11, Bari, 70124, Italy. ericasilvestris85@gmail.com.

ABSTRACT
Infertility is a medical and social condition that affects millions of women worldwide and is today considered so far as a new disease. A considerable progress has been recently pursued in the field of the reproductive medicine and the infertility treatment may account for novel and modern procedures such as in vitro oocyte fertilization, egg donation, pregnancy surrogacy and preimplantation diagnosis. However, great interest has lately been reserved to the ovarian stem cells (OSCs) whose existence in woman ovaries has been proven. OSCs are thus suitable for developmental studies in infertility and in other clinical applications as endocrine derangements due to premature ovarian failure, or for infertility treatment after cancer chemotherapies, as well as in restoring the hormonal balance in postmenopausal age.

No MeSH data available.


Related in: MedlinePlus