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Perspective in infertility: the ovarian stem cells.

Silvestris E, D'Oronzo S, Cafforio P, D'Amato G, Loverro G - J Ovarian Res (2015)

Bottom Line: A considerable progress has been recently pursued in the field of the reproductive medicine and the infertility treatment may account for novel and modern procedures such as in vitro oocyte fertilization, egg donation, pregnancy surrogacy and preimplantation diagnosis.However, great interest has lately been reserved to the ovarian stem cells (OSCs) whose existence in woman ovaries has been proven.OSCs are thus suitable for developmental studies in infertility and in other clinical applications as endocrine derangements due to premature ovarian failure, or for infertility treatment after cancer chemotherapies, as well as in restoring the hormonal balance in postmenopausal age.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Sciences and Human Oncology, Division of Gynecology and Obstetrics, University of Bari 'Aldo Moro', P.za Giulio Cesare, 11, Bari, 70124, Italy. ericasilvestris85@gmail.com.

ABSTRACT
Infertility is a medical and social condition that affects millions of women worldwide and is today considered so far as a new disease. A considerable progress has been recently pursued in the field of the reproductive medicine and the infertility treatment may account for novel and modern procedures such as in vitro oocyte fertilization, egg donation, pregnancy surrogacy and preimplantation diagnosis. However, great interest has lately been reserved to the ovarian stem cells (OSCs) whose existence in woman ovaries has been proven. OSCs are thus suitable for developmental studies in infertility and in other clinical applications as endocrine derangements due to premature ovarian failure, or for infertility treatment after cancer chemotherapies, as well as in restoring the hormonal balance in postmenopausal age.

No MeSH data available.


Related in: MedlinePlus

Structure of human ovary. Left – Schematic representation of the ovarian structure in a woman in reproductive age, showing the evolution of primary follicles to corpora lutea that cyclically occur in the cortex of the organ. Right – The ovarian cortex is the presumable site of the ovarian stem cell location in this ovary preparation after hematoxylin/eosin staining
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Fig1: Structure of human ovary. Left – Schematic representation of the ovarian structure in a woman in reproductive age, showing the evolution of primary follicles to corpora lutea that cyclically occur in the cortex of the organ. Right – The ovarian cortex is the presumable site of the ovarian stem cell location in this ovary preparation after hematoxylin/eosin staining

Mentions: Therefore, Tilly and collaborators validated a FACS-based protocol to purify OSCs from both murine and human ovarian cortex (Fig. 1) as putative location [12]. The investigation was based on the immunological detection of a putative cell surface variant of Ddx-4, commonly considered cytoplasmic, through a rabbit polyclonal antibody against the COOH-terminus of the protein. Once purified by sorting, the cells showed a germline gene expression pattern and were thus established in culture, engineered to express GFP and injected into biopsies of adult human ovarian cortical tissue. The fragments were subsequently xenografted into immunodeficient female mice and new follicles containing GFP-positive oocytes were detected 1–2 weeks later. Major skepticism arose with respect to the choice of using Ddx-4 as cell surface marker used for the OSC sorting since its localization apparently occurs in cytoplasm rather than on plasma membrane [13]. On the other hand, to strengthen the criticism, Zhang and colleagues [14] generated a fluorescent mouse model for both in vivo and in vitro tracing of Ddx-4 expressing OSCs, and in the absence of fluorescence in ovaries they stated that OSCs did not enter in mitosis, nor contributed to the oocyte renewal. Table 2 lists an incomplete panel of markers differentially expressed by OSCs and oocytes.Fig. 1


Perspective in infertility: the ovarian stem cells.

Silvestris E, D'Oronzo S, Cafforio P, D'Amato G, Loverro G - J Ovarian Res (2015)

Structure of human ovary. Left – Schematic representation of the ovarian structure in a woman in reproductive age, showing the evolution of primary follicles to corpora lutea that cyclically occur in the cortex of the organ. Right – The ovarian cortex is the presumable site of the ovarian stem cell location in this ovary preparation after hematoxylin/eosin staining
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4528806&req=5

Fig1: Structure of human ovary. Left – Schematic representation of the ovarian structure in a woman in reproductive age, showing the evolution of primary follicles to corpora lutea that cyclically occur in the cortex of the organ. Right – The ovarian cortex is the presumable site of the ovarian stem cell location in this ovary preparation after hematoxylin/eosin staining
Mentions: Therefore, Tilly and collaborators validated a FACS-based protocol to purify OSCs from both murine and human ovarian cortex (Fig. 1) as putative location [12]. The investigation was based on the immunological detection of a putative cell surface variant of Ddx-4, commonly considered cytoplasmic, through a rabbit polyclonal antibody against the COOH-terminus of the protein. Once purified by sorting, the cells showed a germline gene expression pattern and were thus established in culture, engineered to express GFP and injected into biopsies of adult human ovarian cortical tissue. The fragments were subsequently xenografted into immunodeficient female mice and new follicles containing GFP-positive oocytes were detected 1–2 weeks later. Major skepticism arose with respect to the choice of using Ddx-4 as cell surface marker used for the OSC sorting since its localization apparently occurs in cytoplasm rather than on plasma membrane [13]. On the other hand, to strengthen the criticism, Zhang and colleagues [14] generated a fluorescent mouse model for both in vivo and in vitro tracing of Ddx-4 expressing OSCs, and in the absence of fluorescence in ovaries they stated that OSCs did not enter in mitosis, nor contributed to the oocyte renewal. Table 2 lists an incomplete panel of markers differentially expressed by OSCs and oocytes.Fig. 1

Bottom Line: A considerable progress has been recently pursued in the field of the reproductive medicine and the infertility treatment may account for novel and modern procedures such as in vitro oocyte fertilization, egg donation, pregnancy surrogacy and preimplantation diagnosis.However, great interest has lately been reserved to the ovarian stem cells (OSCs) whose existence in woman ovaries has been proven.OSCs are thus suitable for developmental studies in infertility and in other clinical applications as endocrine derangements due to premature ovarian failure, or for infertility treatment after cancer chemotherapies, as well as in restoring the hormonal balance in postmenopausal age.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Sciences and Human Oncology, Division of Gynecology and Obstetrics, University of Bari 'Aldo Moro', P.za Giulio Cesare, 11, Bari, 70124, Italy. ericasilvestris85@gmail.com.

ABSTRACT
Infertility is a medical and social condition that affects millions of women worldwide and is today considered so far as a new disease. A considerable progress has been recently pursued in the field of the reproductive medicine and the infertility treatment may account for novel and modern procedures such as in vitro oocyte fertilization, egg donation, pregnancy surrogacy and preimplantation diagnosis. However, great interest has lately been reserved to the ovarian stem cells (OSCs) whose existence in woman ovaries has been proven. OSCs are thus suitable for developmental studies in infertility and in other clinical applications as endocrine derangements due to premature ovarian failure, or for infertility treatment after cancer chemotherapies, as well as in restoring the hormonal balance in postmenopausal age.

No MeSH data available.


Related in: MedlinePlus