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Enhanced production of Ca²⁺-polymalate (PMA) with high molecular mass by Aureobasidium pullulans var. pullulans MCW.

Wang YK, Chi Z, Zhou HX, Liu GL, Chi ZM - Microb. Cell Fact. (2015)

Bottom Line: The medium containing only 140.0 g/L glucose, 65.0 g/L CaCO3 and 7.5 g/L corn steep liquor was found to be the most suitable for Ca(2+)-PMA production.During 10-L batch fermentation, 152.52 g/L of Ca(2+)-PMA in the culture and 8.6 g/L of cell dry weight were obtained within 96 h, leaving 4.5 g/L of reducing sugar in the fermented medium.Mw (the apparent molecular weight) of the purified PMA was 2.054 × 10(5) (g/moL) and the purified PMA was estimated to be composed of 1784 L-malic acids.

View Article: PubMed Central - PubMed

Affiliation: College of Marine Life Sciences, Ocean University of China, Yushan Road, No. 5, Qingdao, 266003, China. w84234102@sina.com.

ABSTRACT

Background: Polymalic acid (PMA) has many applications in food and medical industries. However, so far it has not been commercially produced by fermentation. Therefore, it is very important how to develop an economical process for a large scale production of PMA by one step fermentation.

Results: After over 200 strains of Aureobasidium spp. isolated from the mangrove systems in the South of China were screened for their ability to produce Ca(2+)-polymalate (PMA), it was found that Aureobasidium pullulans var. pullulans MCW strain among them could produce high level of Ca(2+)-PMA. The medium containing only 140.0 g/L glucose, 65.0 g/L CaCO3 and 7.5 g/L corn steep liquor was found to be the most suitable for Ca(2+)-PMA production. Then, 121.3 g/L of Ca(2+)-PMA was produced by A. pullulans var. pullulans MCW strain within 120 h at flask level. During 10-L batch fermentation, 152.52 g/L of Ca(2+)-PMA in the culture and 8.6 g/L of cell dry weight were obtained within 96 h, leaving 4.5 g/L of reducing sugar in the fermented medium. After purification of the Ca(2+)-PMA from the culture and acid hydrolysis of the purified Ca(2+)-PMA, HPLC analysis showed that A. pullulans var. pullulans MCW strain produced only one main component of Ca(2+)-PMA and the hydrolysate of the purified Ca(2+)-PMA was mainly composed of L-malic acid. Mw (the apparent molecular weight) of the purified PMA was 2.054 × 10(5) (g/moL) and the purified PMA was estimated to be composed of 1784 L-malic acids.

Conclusions: It was found that A. pullulans var. pullulans MCW strain obtained in this study could yield 152.52 g/L of Ca(2+)-PMA within the short time, the produced PMA had the highest molecular weight and the medium for production of Ca(2+)- PMA by this yeast was very simple.

No MeSH data available.


Related in: MedlinePlus

HPLC chromatogram of standard calcium malate and malic acid (c), fermentation product (a), and hydrolysate of the fermentation product using H2SO4 as the acid (b).
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Fig6: HPLC chromatogram of standard calcium malate and malic acid (c), fermentation product (a), and hydrolysate of the fermentation product using H2SO4 as the acid (b).

Mentions: After the analysis of the purified precipitate with HPLC, the results in Fig. 6a confirmed that the major fermentation product was composed of one component and the minor component was calcium malate, the precursor of PMA, indicating that the main product produced by the yeast strain MCW was Ca2+-PMA. It also can be seen from the results in Fig. 6b that after acid hydrolysis of the fermentation product, the hydrolysates were composed of major l-malic acid and minor calcium malate and Ca2+-PMA. In contrast, the yeast strain P6 produced two components of Ca2+-PMA, and the hydrolysate of the Ca2+-PMA only contained calcium malate [13]. However, after the acid hydrolysis of the fermentation products produced by A. pullulans strain ZX-10, the hydrolysates contained acetic acid and malic acid [10]. This again confirmed that A. pullulans var. pullulans MCW strain used in this study may be the most suitable yeast strain for Ca2+-PMA production from glucose on a large scale in industry.Fig. 6


Enhanced production of Ca²⁺-polymalate (PMA) with high molecular mass by Aureobasidium pullulans var. pullulans MCW.

Wang YK, Chi Z, Zhou HX, Liu GL, Chi ZM - Microb. Cell Fact. (2015)

HPLC chromatogram of standard calcium malate and malic acid (c), fermentation product (a), and hydrolysate of the fermentation product using H2SO4 as the acid (b).
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4528779&req=5

Fig6: HPLC chromatogram of standard calcium malate and malic acid (c), fermentation product (a), and hydrolysate of the fermentation product using H2SO4 as the acid (b).
Mentions: After the analysis of the purified precipitate with HPLC, the results in Fig. 6a confirmed that the major fermentation product was composed of one component and the minor component was calcium malate, the precursor of PMA, indicating that the main product produced by the yeast strain MCW was Ca2+-PMA. It also can be seen from the results in Fig. 6b that after acid hydrolysis of the fermentation product, the hydrolysates were composed of major l-malic acid and minor calcium malate and Ca2+-PMA. In contrast, the yeast strain P6 produced two components of Ca2+-PMA, and the hydrolysate of the Ca2+-PMA only contained calcium malate [13]. However, after the acid hydrolysis of the fermentation products produced by A. pullulans strain ZX-10, the hydrolysates contained acetic acid and malic acid [10]. This again confirmed that A. pullulans var. pullulans MCW strain used in this study may be the most suitable yeast strain for Ca2+-PMA production from glucose on a large scale in industry.Fig. 6

Bottom Line: The medium containing only 140.0 g/L glucose, 65.0 g/L CaCO3 and 7.5 g/L corn steep liquor was found to be the most suitable for Ca(2+)-PMA production.During 10-L batch fermentation, 152.52 g/L of Ca(2+)-PMA in the culture and 8.6 g/L of cell dry weight were obtained within 96 h, leaving 4.5 g/L of reducing sugar in the fermented medium.Mw (the apparent molecular weight) of the purified PMA was 2.054 × 10(5) (g/moL) and the purified PMA was estimated to be composed of 1784 L-malic acids.

View Article: PubMed Central - PubMed

Affiliation: College of Marine Life Sciences, Ocean University of China, Yushan Road, No. 5, Qingdao, 266003, China. w84234102@sina.com.

ABSTRACT

Background: Polymalic acid (PMA) has many applications in food and medical industries. However, so far it has not been commercially produced by fermentation. Therefore, it is very important how to develop an economical process for a large scale production of PMA by one step fermentation.

Results: After over 200 strains of Aureobasidium spp. isolated from the mangrove systems in the South of China were screened for their ability to produce Ca(2+)-polymalate (PMA), it was found that Aureobasidium pullulans var. pullulans MCW strain among them could produce high level of Ca(2+)-PMA. The medium containing only 140.0 g/L glucose, 65.0 g/L CaCO3 and 7.5 g/L corn steep liquor was found to be the most suitable for Ca(2+)-PMA production. Then, 121.3 g/L of Ca(2+)-PMA was produced by A. pullulans var. pullulans MCW strain within 120 h at flask level. During 10-L batch fermentation, 152.52 g/L of Ca(2+)-PMA in the culture and 8.6 g/L of cell dry weight were obtained within 96 h, leaving 4.5 g/L of reducing sugar in the fermented medium. After purification of the Ca(2+)-PMA from the culture and acid hydrolysis of the purified Ca(2+)-PMA, HPLC analysis showed that A. pullulans var. pullulans MCW strain produced only one main component of Ca(2+)-PMA and the hydrolysate of the purified Ca(2+)-PMA was mainly composed of L-malic acid. Mw (the apparent molecular weight) of the purified PMA was 2.054 × 10(5) (g/moL) and the purified PMA was estimated to be composed of 1784 L-malic acids.

Conclusions: It was found that A. pullulans var. pullulans MCW strain obtained in this study could yield 152.52 g/L of Ca(2+)-PMA within the short time, the produced PMA had the highest molecular weight and the medium for production of Ca(2+)- PMA by this yeast was very simple.

No MeSH data available.


Related in: MedlinePlus