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Diverse origins of hepatitis C virus in HIV co-infected men who have sex with men in Hong Kong.

Chan DP, Lin AW, Wong KH, Wong NS, Lee SS - Virol. J. (2015)

Bottom Line: Detection and molecular characterization of HCV was successfully performed in 22 (91.7 %) patients.There was otherwise no temporal or genetic clustering of the corresponding HIV sequences.The transmission dynamics of HIV and HCV infections in MSM in Hong Kong were evidently unrelated.

View Article: PubMed Central - PubMed

Affiliation: Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong, China. denisechan@cuhk.edu.hk.

ABSTRACT

Background: Worldwide, Hepatitis C (HCV) infection has been increasingly recognized in HIV-positive men who have sex with men (MSM). The objective of this study was to characterize the transmission dynamics of acute HCV infection in HIV-positive MSM in Hong Kong using a molecular approach.

Findings: We retrospectively examined 24 HIV-positive MSM with acute HCV infection diagnosed between 2009 and 2014 in Hong Kong. Detection and molecular characterization of HCV was successfully performed in 22 (91.7 %) patients. Genotype 3a was the most prevalent as identified in 14 (63.6 %) MSM, followed by 1a in 4 (18.2 %), 6a in 2 (9.1 %), and 1each (4.5 %) for 1b and 2a. The high prevalence of genotype 3a in MSM was in stark contrast to its rarity among HCV infected injection drug users (IDU) in Hong Kong. Phylogenetic analyses revealed a monophyletic HCV-3a cluster composing of MSM without injection history, and a homologous pair with HCV-6a genotype. There was otherwise no temporal or genetic clustering of the corresponding HIV sequences.

Conclusions: The origin of sexually acquired acute HCV infections in HIV-positive MSM was diverse and not directly linked with local IDU. The transmission dynamics of HIV and HCV infections in MSM in Hong Kong were evidently unrelated.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic analyses of nucleotide sequences of the partial NS5B gene of HCV-3a (a) and HCV-6a (b). The trees were constructed by the neighbor-joining method based on Kimura-2 parameter model. Bootstrap values of >70 are given at branch nodes. Representative strains obtained from GenBank are identified by accession number, strain name, country of origin and year of isolation. HCV-1a is included as outgroup. Solid circles represent the HCV strains isolated in this study. Scale bars indicate nucleotide substitutions per site
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Fig3: Phylogenetic analyses of nucleotide sequences of the partial NS5B gene of HCV-3a (a) and HCV-6a (b). The trees were constructed by the neighbor-joining method based on Kimura-2 parameter model. Bootstrap values of >70 are given at branch nodes. Representative strains obtained from GenBank are identified by accession number, strain name, country of origin and year of isolation. HCV-1a is included as outgroup. Solid circles represent the HCV strains isolated in this study. Scale bars indicate nucleotide substitutions per site

Mentions: Figure 3a shows the results of phylogenetic analysis for the HCV genotype 3a samples. The HCV-3a sequences were distinctly different from those obtained from IDU in Hong Kong and Mainland China. The considerably shorter genetic distance between sequences within the HCV-3a cluster and their low genetic diversity, with diagnosis made within 2 years, suggested the occurrence of rapid virus spread in the HIV + ve MSM community, which might have resulted from a single source of recent introduction within Hong Kong. The origin of this cluster could not be further characterized as no similar sequences in Hong Kong could be found. HCV transmission might have arisen from the spillover of the virus from IDU to an MSM long time ago, as the sequences did not show any similarity with those in China, Southeast Asia or Australia. In Australia, genotype 3a actually constituted one of the most prevalent virus strains in acute HCV infections. Unlike the situation in Hong Kong, however, overlapping and coexistence of HCV-3a clusters between the IDU and HIV + ve MSM populations could be demonstrated in Australia [10].Fig. 3


Diverse origins of hepatitis C virus in HIV co-infected men who have sex with men in Hong Kong.

Chan DP, Lin AW, Wong KH, Wong NS, Lee SS - Virol. J. (2015)

Phylogenetic analyses of nucleotide sequences of the partial NS5B gene of HCV-3a (a) and HCV-6a (b). The trees were constructed by the neighbor-joining method based on Kimura-2 parameter model. Bootstrap values of >70 are given at branch nodes. Representative strains obtained from GenBank are identified by accession number, strain name, country of origin and year of isolation. HCV-1a is included as outgroup. Solid circles represent the HCV strains isolated in this study. Scale bars indicate nucleotide substitutions per site
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4528697&req=5

Fig3: Phylogenetic analyses of nucleotide sequences of the partial NS5B gene of HCV-3a (a) and HCV-6a (b). The trees were constructed by the neighbor-joining method based on Kimura-2 parameter model. Bootstrap values of >70 are given at branch nodes. Representative strains obtained from GenBank are identified by accession number, strain name, country of origin and year of isolation. HCV-1a is included as outgroup. Solid circles represent the HCV strains isolated in this study. Scale bars indicate nucleotide substitutions per site
Mentions: Figure 3a shows the results of phylogenetic analysis for the HCV genotype 3a samples. The HCV-3a sequences were distinctly different from those obtained from IDU in Hong Kong and Mainland China. The considerably shorter genetic distance between sequences within the HCV-3a cluster and their low genetic diversity, with diagnosis made within 2 years, suggested the occurrence of rapid virus spread in the HIV + ve MSM community, which might have resulted from a single source of recent introduction within Hong Kong. The origin of this cluster could not be further characterized as no similar sequences in Hong Kong could be found. HCV transmission might have arisen from the spillover of the virus from IDU to an MSM long time ago, as the sequences did not show any similarity with those in China, Southeast Asia or Australia. In Australia, genotype 3a actually constituted one of the most prevalent virus strains in acute HCV infections. Unlike the situation in Hong Kong, however, overlapping and coexistence of HCV-3a clusters between the IDU and HIV + ve MSM populations could be demonstrated in Australia [10].Fig. 3

Bottom Line: Detection and molecular characterization of HCV was successfully performed in 22 (91.7 %) patients.There was otherwise no temporal or genetic clustering of the corresponding HIV sequences.The transmission dynamics of HIV and HCV infections in MSM in Hong Kong were evidently unrelated.

View Article: PubMed Central - PubMed

Affiliation: Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong, China. denisechan@cuhk.edu.hk.

ABSTRACT

Background: Worldwide, Hepatitis C (HCV) infection has been increasingly recognized in HIV-positive men who have sex with men (MSM). The objective of this study was to characterize the transmission dynamics of acute HCV infection in HIV-positive MSM in Hong Kong using a molecular approach.

Findings: We retrospectively examined 24 HIV-positive MSM with acute HCV infection diagnosed between 2009 and 2014 in Hong Kong. Detection and molecular characterization of HCV was successfully performed in 22 (91.7 %) patients. Genotype 3a was the most prevalent as identified in 14 (63.6 %) MSM, followed by 1a in 4 (18.2 %), 6a in 2 (9.1 %), and 1each (4.5 %) for 1b and 2a. The high prevalence of genotype 3a in MSM was in stark contrast to its rarity among HCV infected injection drug users (IDU) in Hong Kong. Phylogenetic analyses revealed a monophyletic HCV-3a cluster composing of MSM without injection history, and a homologous pair with HCV-6a genotype. There was otherwise no temporal or genetic clustering of the corresponding HIV sequences.

Conclusions: The origin of sexually acquired acute HCV infections in HIV-positive MSM was diverse and not directly linked with local IDU. The transmission dynamics of HIV and HCV infections in MSM in Hong Kong were evidently unrelated.

No MeSH data available.


Related in: MedlinePlus