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Determination of growth stages and metabolic profiles in Brachypodium distachyon for comparison of developmental context with Triticeae crops.

Onda Y, Hashimoto K, Yoshida T, Sakurai T, Sawada Y, Hirai MY, Toyooka K, Mochida K, Shinozaki K - Proc. Biol. Sci. (2015)

Bottom Line: Furthermore, we compared metabolite accumulation patterns across different growth stages and across different stress conditions using a widely targeted metabolome analysis.Metabolic profiling determined commonalities and specificities in chemical properties that were dependent on organisms, growth stages and/or stress conditions.Most metabolites accumulated equivalently in B. distachyon and wheat.

View Article: PubMed Central - PubMed

Affiliation: Cellulose Production Research Team, RIKEN Center for Sustainable Resource Science, Kanagawa, Japan Kihara Institute for Biological Research, Yokohama City University, Kanagawa, Japan.

No MeSH data available.


Related in: MedlinePlus

Metabolite profiling of the leaves of Bd21, Bd3-1 and Chinese Spring wheat under stress conditions. Dendrogram and heat map show metabolite accumulation, clustered based on the binary logarithm (log2) value of quantitative data obtained by UPLC-TQS. Only the first leaves were used.
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RSPB20150964F4: Metabolite profiling of the leaves of Bd21, Bd3-1 and Chinese Spring wheat under stress conditions. Dendrogram and heat map show metabolite accumulation, clustered based on the binary logarithm (log2) value of quantitative data obtained by UPLC-TQS. Only the first leaves were used.

Mentions: Next, we conducted further metabolome analyses under stress conditions (2°C, 12°C, 32°C, 42°C, 100 mM NaCl and 500 mM NaCl) using plants at the BBCH15 stage. In total, 491 metabolites were detected in the leaf samples (electronic supplementary material, table S5). Metabolites that showed S/N ratios of more than 10 in at least one sample among 21 (three plant types, seven stress conditions) were selected (indicated with asterisks in electronic supplementary material, table S5), and a data matrix containing 145 metabolites was created (electronic supplementary material, table S6). Hierarchical clustering analysis revealed two major clusters (figure 4, cluster A and B). Cluster A contained metabolites with relatively lower accumulation among all subjected samples, whereas cluster B contained metabolites with relatively higher accumulation. The hierarchical clustering analysis also grouped samples separately into B. distachyon and wheat (figure 4, cluster C and D). This separation of these two species at BBCH15 is consistent with the results of the hierarchical clustering analysis at BBCH13 as described above. Although samples of Bd21 subjected to the 2°C, 42°C, 100 mM NaCl and 500 mM NaCl treatments were grouped with those of Bd3-1 in the same cluster, Bd21 samples subjected to control, 12 and 32°C treatments were grouped separately from those of Bd3-1 (figure 4, enclosed by green, blue and red lines, respectively). These results suggest that Bd21 and Bd3-1 showed similar metabolite accumulation profiles as B. distachyon under more stringent stress conditions, whereas they showed different profiles as different accessions under milder stress conditions. To summarize the commonalities and specificities of metabolome profiling, metabolites that showed an S/N ratio of more than 10 were visualized in a Venn diagram under each stress condition (electronic supplementary material, figure S5). The majority of the metabolites accumulated among Bd21, Bd3-1 and wheat under all treatments; these commonly accumulated metabolites are listed in electronic supplementary material, table S7.Figure 4.


Determination of growth stages and metabolic profiles in Brachypodium distachyon for comparison of developmental context with Triticeae crops.

Onda Y, Hashimoto K, Yoshida T, Sakurai T, Sawada Y, Hirai MY, Toyooka K, Mochida K, Shinozaki K - Proc. Biol. Sci. (2015)

Metabolite profiling of the leaves of Bd21, Bd3-1 and Chinese Spring wheat under stress conditions. Dendrogram and heat map show metabolite accumulation, clustered based on the binary logarithm (log2) value of quantitative data obtained by UPLC-TQS. Only the first leaves were used.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4528556&req=5

RSPB20150964F4: Metabolite profiling of the leaves of Bd21, Bd3-1 and Chinese Spring wheat under stress conditions. Dendrogram and heat map show metabolite accumulation, clustered based on the binary logarithm (log2) value of quantitative data obtained by UPLC-TQS. Only the first leaves were used.
Mentions: Next, we conducted further metabolome analyses under stress conditions (2°C, 12°C, 32°C, 42°C, 100 mM NaCl and 500 mM NaCl) using plants at the BBCH15 stage. In total, 491 metabolites were detected in the leaf samples (electronic supplementary material, table S5). Metabolites that showed S/N ratios of more than 10 in at least one sample among 21 (three plant types, seven stress conditions) were selected (indicated with asterisks in electronic supplementary material, table S5), and a data matrix containing 145 metabolites was created (electronic supplementary material, table S6). Hierarchical clustering analysis revealed two major clusters (figure 4, cluster A and B). Cluster A contained metabolites with relatively lower accumulation among all subjected samples, whereas cluster B contained metabolites with relatively higher accumulation. The hierarchical clustering analysis also grouped samples separately into B. distachyon and wheat (figure 4, cluster C and D). This separation of these two species at BBCH15 is consistent with the results of the hierarchical clustering analysis at BBCH13 as described above. Although samples of Bd21 subjected to the 2°C, 42°C, 100 mM NaCl and 500 mM NaCl treatments were grouped with those of Bd3-1 in the same cluster, Bd21 samples subjected to control, 12 and 32°C treatments were grouped separately from those of Bd3-1 (figure 4, enclosed by green, blue and red lines, respectively). These results suggest that Bd21 and Bd3-1 showed similar metabolite accumulation profiles as B. distachyon under more stringent stress conditions, whereas they showed different profiles as different accessions under milder stress conditions. To summarize the commonalities and specificities of metabolome profiling, metabolites that showed an S/N ratio of more than 10 were visualized in a Venn diagram under each stress condition (electronic supplementary material, figure S5). The majority of the metabolites accumulated among Bd21, Bd3-1 and wheat under all treatments; these commonly accumulated metabolites are listed in electronic supplementary material, table S7.Figure 4.

Bottom Line: Furthermore, we compared metabolite accumulation patterns across different growth stages and across different stress conditions using a widely targeted metabolome analysis.Metabolic profiling determined commonalities and specificities in chemical properties that were dependent on organisms, growth stages and/or stress conditions.Most metabolites accumulated equivalently in B. distachyon and wheat.

View Article: PubMed Central - PubMed

Affiliation: Cellulose Production Research Team, RIKEN Center for Sustainable Resource Science, Kanagawa, Japan Kihara Institute for Biological Research, Yokohama City University, Kanagawa, Japan.

No MeSH data available.


Related in: MedlinePlus