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Decrease expression and clinicopathological significance of miR-148a with poor survival in hepatocellular carcinoma tissues.

Ajdarkosh H, Dadpay M, Yahaghi E, Pirzaman ER, Fayyaz AF, Darian EK, Mokarizadeh A - Diagn Pathol (2015)

Bottom Line: The result showed that reduction of miR-148a expression was associated with TNM stage, metastasis, and number of tumor nodes.Moreover, our result showed that lower expression in tumor tissues in comparison with corresponding normal control tissues.MicroRNA-148a may act as a suppressor miRNA of HCC, and it is therefore a potential prognostic biomarker for HCC patients.

View Article: PubMed Central - PubMed

Affiliation: Gastrointestinal and Liver Disease Research Center (GILDRC), Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death worldwide, mainly due to its high rates of postoperative recurrence and metastasis. Please remove, it currently ranks as the third most common cause of cancer-related deaths. MiRNAs are a set of small, single-stranded, non-coding RNA molecules that negatively regulate gene expression at the post-transcriptional level. In this study, we demonstrated the down-regulation of miR-148a in HCC and non-cancerous tissues using qRT-PCR.

Methods: Ninety six HCC samples and their noncancerous normal liver tissues were collected. Total mRNA including miRNA was extracted, and miR-148a expression was determined using qRT-PCR. Furthermore, the correlation between the miR-148a expression and clinicopathological parameters was investigated.

Results: The result showed that reduction of miR-148a expression was associated with TNM stage, metastasis, and number of tumor nodes. Multivariate Cox proportional hazards model analysis showed that low expression of miR-148a was independently associated with recurrence of HCC in the current study. Moreover, our result showed that lower expression in tumor tissues in comparison with corresponding normal control tissues.

Conclusion: Down-regulation of miR-148a is related to HCC carcinogenesis and deterioration of HCC. MicroRNA-148a may act as a suppressor miRNA of HCC, and it is therefore a potential prognostic biomarker for HCC patients.

No MeSH data available.


Related in: MedlinePlus

The area under the curve (AUC) of miR-148a with the sensitivity and specificity were calculated to be 80 % and 62.2 %, respectively
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Fig1: The area under the curve (AUC) of miR-148a with the sensitivity and specificity were calculated to be 80 % and 62.2 %, respectively

Mentions: Significantly decreased expression of miR-148a was observed in the HCC tissues than in the adjacent normal hepatic tissues. ROC analysis was used to evaluate the diagnostic value of miR-148a. The area under the curve (AUC) of miR-148a was 0.837 (95 % CI 0.782 - 0.954, P < 0.035). In addition, the median 2-Δcq 0.93 was calculated as the cut-off value of miR-148a. As shown in Fig. 1, the sensitivity and specificity were calculated to be 80 % and 62.2 %, respectively. Furthermore, results suggested that expression of miR-148a in advanced stages (III and IV) was remarkably low than that in early stages (I and II), (P = 0.042). Low expression was also associated with metastasis (P = 0.021). However, miR-148a expression was not associated with sex (P = 0.712), age (P = 0.462), Tumor diameter (P = 0.316), liver cirrhosis (0.621), and differentiation (P = 0.412), (Table 1). The result indicated that the patients with low expression of miR-148a had a longer time-to-recurrence when compared with low expression patients (1.47 ± 0.42; 2.87 ± 0.64 mean ± SD), Nevertheless, there was no significant difference between two groups (P = 0.417). Multivariate Cox proportional hazards model analysis showed that low expression of miR-148a was independently associated with recurrence of HCC (HR = 2.68; 95 % CI: 1.416-8.367, P = 0.026).Fig. 1


Decrease expression and clinicopathological significance of miR-148a with poor survival in hepatocellular carcinoma tissues.

Ajdarkosh H, Dadpay M, Yahaghi E, Pirzaman ER, Fayyaz AF, Darian EK, Mokarizadeh A - Diagn Pathol (2015)

The area under the curve (AUC) of miR-148a with the sensitivity and specificity were calculated to be 80 % and 62.2 %, respectively
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4528397&req=5

Fig1: The area under the curve (AUC) of miR-148a with the sensitivity and specificity were calculated to be 80 % and 62.2 %, respectively
Mentions: Significantly decreased expression of miR-148a was observed in the HCC tissues than in the adjacent normal hepatic tissues. ROC analysis was used to evaluate the diagnostic value of miR-148a. The area under the curve (AUC) of miR-148a was 0.837 (95 % CI 0.782 - 0.954, P < 0.035). In addition, the median 2-Δcq 0.93 was calculated as the cut-off value of miR-148a. As shown in Fig. 1, the sensitivity and specificity were calculated to be 80 % and 62.2 %, respectively. Furthermore, results suggested that expression of miR-148a in advanced stages (III and IV) was remarkably low than that in early stages (I and II), (P = 0.042). Low expression was also associated with metastasis (P = 0.021). However, miR-148a expression was not associated with sex (P = 0.712), age (P = 0.462), Tumor diameter (P = 0.316), liver cirrhosis (0.621), and differentiation (P = 0.412), (Table 1). The result indicated that the patients with low expression of miR-148a had a longer time-to-recurrence when compared with low expression patients (1.47 ± 0.42; 2.87 ± 0.64 mean ± SD), Nevertheless, there was no significant difference between two groups (P = 0.417). Multivariate Cox proportional hazards model analysis showed that low expression of miR-148a was independently associated with recurrence of HCC (HR = 2.68; 95 % CI: 1.416-8.367, P = 0.026).Fig. 1

Bottom Line: The result showed that reduction of miR-148a expression was associated with TNM stage, metastasis, and number of tumor nodes.Moreover, our result showed that lower expression in tumor tissues in comparison with corresponding normal control tissues.MicroRNA-148a may act as a suppressor miRNA of HCC, and it is therefore a potential prognostic biomarker for HCC patients.

View Article: PubMed Central - PubMed

Affiliation: Gastrointestinal and Liver Disease Research Center (GILDRC), Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death worldwide, mainly due to its high rates of postoperative recurrence and metastasis. Please remove, it currently ranks as the third most common cause of cancer-related deaths. MiRNAs are a set of small, single-stranded, non-coding RNA molecules that negatively regulate gene expression at the post-transcriptional level. In this study, we demonstrated the down-regulation of miR-148a in HCC and non-cancerous tissues using qRT-PCR.

Methods: Ninety six HCC samples and their noncancerous normal liver tissues were collected. Total mRNA including miRNA was extracted, and miR-148a expression was determined using qRT-PCR. Furthermore, the correlation between the miR-148a expression and clinicopathological parameters was investigated.

Results: The result showed that reduction of miR-148a expression was associated with TNM stage, metastasis, and number of tumor nodes. Multivariate Cox proportional hazards model analysis showed that low expression of miR-148a was independently associated with recurrence of HCC in the current study. Moreover, our result showed that lower expression in tumor tissues in comparison with corresponding normal control tissues.

Conclusion: Down-regulation of miR-148a is related to HCC carcinogenesis and deterioration of HCC. MicroRNA-148a may act as a suppressor miRNA of HCC, and it is therefore a potential prognostic biomarker for HCC patients.

No MeSH data available.


Related in: MedlinePlus