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The Effects of Fenugreek on Radiation Induced Toxicity for Human Blood T-Cells in Radiotherapy.

Tavakoli MB, Kiani A, Roayaei M - J Med Signals Sens (2015 Jul-Sep)

Bottom Line: T-cells peripheral blood lymphocyte isolated from blood samples of healthy individuals with no irradiated job background.The media of cultured cells was irradiated 1-h after the fenugreek extract was added.An obvious variation in apoptotic cells number was observed in presence of fenugreek extract (>80%).

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Physics, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran.

ABSTRACT
Many cellular damages either in normal or cancerous tissues are the outcome of molecular events affected by ionizing radiation. T-cells are the most important among immune system agents and are used for biological radiation dose measurement in recommended standard methods. The herbs with immune modulating properties may be useful to reduce the risk of the damages and subsequently the diseases. The T-cells as the most important immune cells being targeted for biological dosimetry of radiation. This study proposes a flowcytometric-method based on fluorescein isothiocyanate- and propidium iodide (PI)-labeled annexin-V to assess apoptosis in blood T-cells after irradiation in both presence and absence of fenugreek extract. T-cells peripheral blood lymphocyte isolated from blood samples of healthy individuals with no irradiated job background. The media of cultured cells was irradiated 1-h after the fenugreek extract was added. The number of apoptotic cells was assessed by annexin-V protocol and multicolor flowcytometry. An obvious variation in apoptotic cells number was observed in presence of fenugreek extract (>80%). The results suggest that fenugreek extract can potentiate the radiation induced apoptosis or radiation toxicity in blood T-cells (P < 0.05).

No MeSH data available.


Related in: MedlinePlus

Extracted lymphocyte under microscope vision
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Figure 1: Extracted lymphocyte under microscope vision

Mentions: Cell culture and irradiation were done by standard preparation strategies previously described in International Atomic Energy Agency Technical Publication.[26] All blood samples were diluted with a ratio of 1:1 in phosphate buffer saline (PBS) (Sigma–Aldrich GMBH Munich, Germany) and peripheral blood lymphocytes (PBLCs) were separated by density gradient centrifugation using Ficoll-Paque Plus (Sigma–Aldrich GMBH Munich, Germany).[27] The isolated cells were added to Roswell Park Memorial Institute Medium 1640 culture media that was enriched by fetal bovine serum, glutamine, antibiotics, and phytohematoglutinin [Figure 1]. The cultured cells were incubated at 37°C in a 5% CO2 atmosphere.


The Effects of Fenugreek on Radiation Induced Toxicity for Human Blood T-Cells in Radiotherapy.

Tavakoli MB, Kiani A, Roayaei M - J Med Signals Sens (2015 Jul-Sep)

Extracted lymphocyte under microscope vision
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4528356&req=5

Figure 1: Extracted lymphocyte under microscope vision
Mentions: Cell culture and irradiation were done by standard preparation strategies previously described in International Atomic Energy Agency Technical Publication.[26] All blood samples were diluted with a ratio of 1:1 in phosphate buffer saline (PBS) (Sigma–Aldrich GMBH Munich, Germany) and peripheral blood lymphocytes (PBLCs) were separated by density gradient centrifugation using Ficoll-Paque Plus (Sigma–Aldrich GMBH Munich, Germany).[27] The isolated cells were added to Roswell Park Memorial Institute Medium 1640 culture media that was enriched by fetal bovine serum, glutamine, antibiotics, and phytohematoglutinin [Figure 1]. The cultured cells were incubated at 37°C in a 5% CO2 atmosphere.

Bottom Line: T-cells peripheral blood lymphocyte isolated from blood samples of healthy individuals with no irradiated job background.The media of cultured cells was irradiated 1-h after the fenugreek extract was added.An obvious variation in apoptotic cells number was observed in presence of fenugreek extract (>80%).

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Physics, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran.

ABSTRACT
Many cellular damages either in normal or cancerous tissues are the outcome of molecular events affected by ionizing radiation. T-cells are the most important among immune system agents and are used for biological radiation dose measurement in recommended standard methods. The herbs with immune modulating properties may be useful to reduce the risk of the damages and subsequently the diseases. The T-cells as the most important immune cells being targeted for biological dosimetry of radiation. This study proposes a flowcytometric-method based on fluorescein isothiocyanate- and propidium iodide (PI)-labeled annexin-V to assess apoptosis in blood T-cells after irradiation in both presence and absence of fenugreek extract. T-cells peripheral blood lymphocyte isolated from blood samples of healthy individuals with no irradiated job background. The media of cultured cells was irradiated 1-h after the fenugreek extract was added. The number of apoptotic cells was assessed by annexin-V protocol and multicolor flowcytometry. An obvious variation in apoptotic cells number was observed in presence of fenugreek extract (>80%). The results suggest that fenugreek extract can potentiate the radiation induced apoptosis or radiation toxicity in blood T-cells (P < 0.05).

No MeSH data available.


Related in: MedlinePlus