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Comparative Evolution of Duplicated Ddx3 Genes in Teleosts: Insights from Japanese Flounder, Paralichthys olivaceus.

Wang Z, Liu W, Song H, Wang H, Liu J, Zhao H, Du X, Zhang Q - G3 (Bethesda) (2015)

Bottom Line: We confirmed that the two genes originated from teleost-specific genome duplication through synteny and phylogenetic analysis.Additionally, comparative analysis of genome structure, molecular evolution rate, and expression pattern of the two genes in Japanese flounder revealed evidence of subfunctionalization of the duplicated Ddx3 genes in teleosts.Thus, the results of this study reveal novel insights into the evolution of the teleost Ddx3 genes and constitute important groundwork for further research on this gene family.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Marine Genetics and Breeding (MGB), Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.

No MeSH data available.


Related in: MedlinePlus

Tissue distribution patterns of Japanese flounder Ddx3 genes. (A) Relative expression levels of Ddx3 genes in female tissues. (B) Relative expression levels of Ddx3 genes in male tissues. Relative expression level of Ddx3a in the testis was used as calibrator. Data are shown as mean ± SD (n = 5). Values with different superscripts indicate statistical significance (P < 0.05), which was calculated by one-way analysis of variance.
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fig4: Tissue distribution patterns of Japanese flounder Ddx3 genes. (A) Relative expression levels of Ddx3 genes in female tissues. (B) Relative expression levels of Ddx3 genes in male tissues. Relative expression level of Ddx3a in the testis was used as calibrator. Data are shown as mean ± SD (n = 5). Values with different superscripts indicate statistical significance (P < 0.05), which was calculated by one-way analysis of variance.

Mentions: Quantitative real-time PCR results indicated that Ddx3a and Ddx3b showed differential tissue-specific expression pattern in Japanese flounder. In females (Figure 4A), they both showed very high relative expression levels in the ovary, with the level of Ddx3a being much greater than that of Ddx3b. In addition, the relative expression level of Ddx3a was also greater than that of Ddx3b in gills, intestine, kidney, and liver, but lower in the brain. In males (Figure 4B), Ddx3a had the greatest relative expression level in gills, whereas Ddx3b expression was the greatest in testes. The relative expression level of Ddx3b was greater than that of Ddx3a in testes and the brain, whereas that of Ddx3a was higher in gills, intestine, kidney, and liver.


Comparative Evolution of Duplicated Ddx3 Genes in Teleosts: Insights from Japanese Flounder, Paralichthys olivaceus.

Wang Z, Liu W, Song H, Wang H, Liu J, Zhao H, Du X, Zhang Q - G3 (Bethesda) (2015)

Tissue distribution patterns of Japanese flounder Ddx3 genes. (A) Relative expression levels of Ddx3 genes in female tissues. (B) Relative expression levels of Ddx3 genes in male tissues. Relative expression level of Ddx3a in the testis was used as calibrator. Data are shown as mean ± SD (n = 5). Values with different superscripts indicate statistical significance (P < 0.05), which was calculated by one-way analysis of variance.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4528332&req=5

fig4: Tissue distribution patterns of Japanese flounder Ddx3 genes. (A) Relative expression levels of Ddx3 genes in female tissues. (B) Relative expression levels of Ddx3 genes in male tissues. Relative expression level of Ddx3a in the testis was used as calibrator. Data are shown as mean ± SD (n = 5). Values with different superscripts indicate statistical significance (P < 0.05), which was calculated by one-way analysis of variance.
Mentions: Quantitative real-time PCR results indicated that Ddx3a and Ddx3b showed differential tissue-specific expression pattern in Japanese flounder. In females (Figure 4A), they both showed very high relative expression levels in the ovary, with the level of Ddx3a being much greater than that of Ddx3b. In addition, the relative expression level of Ddx3a was also greater than that of Ddx3b in gills, intestine, kidney, and liver, but lower in the brain. In males (Figure 4B), Ddx3a had the greatest relative expression level in gills, whereas Ddx3b expression was the greatest in testes. The relative expression level of Ddx3b was greater than that of Ddx3a in testes and the brain, whereas that of Ddx3a was higher in gills, intestine, kidney, and liver.

Bottom Line: We confirmed that the two genes originated from teleost-specific genome duplication through synteny and phylogenetic analysis.Additionally, comparative analysis of genome structure, molecular evolution rate, and expression pattern of the two genes in Japanese flounder revealed evidence of subfunctionalization of the duplicated Ddx3 genes in teleosts.Thus, the results of this study reveal novel insights into the evolution of the teleost Ddx3 genes and constitute important groundwork for further research on this gene family.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Marine Genetics and Breeding (MGB), Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.

No MeSH data available.


Related in: MedlinePlus