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PUF-8 Functions Redundantly with GLD-1 to Promote the Meiotic Progression of Spermatocytes in Caenorhabditis elegans.

Priti A, Subramaniam K - G3 (Bethesda) (2015)

Bottom Line: During larval development, gld-1; puf-8 double-mutant germ cells begin to express the meiotic marker HIM-3, lose P granules, and form the sperm-specific membranous organelle, which are characteristics of developing spermatocytes.However, some of these cells quickly lose HIM-3 and form germ cell tumors that lack membranous organelle but contain P granules.Together, results presented here uncover a novel function for gld-1 in the meiotic development of spermatocytes in both hermaphrodites and males.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences & Bioengineering, Indian Institute of Technology - Kanpur, Kanpur 208016, India.

No MeSH data available.


Related in: MedlinePlus

Comparison of germ cell tumors between gld-1(-) and gld-1(-);puf-8(-) hermaphrodite germlines. Hermaphrodite gonads of the indicated genotypes stained with DAPI. Mitotically cycling population of germline stem cells is present at the distal end of the gonad. Successive temporal stages of differentiating germ cells are present consecutively in the distal-to-proximal axis with the fully-developed gametes at the proximal end. Sperm nuclei appear as tiny dots within the marked area. Whereas the tumor is on the distal side of sperm in the gld-1 single mutant, it is on the proximal side of sperm in the gld-1; puf-8 double mutant.
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fig3: Comparison of germ cell tumors between gld-1(-) and gld-1(-);puf-8(-) hermaphrodite germlines. Hermaphrodite gonads of the indicated genotypes stained with DAPI. Mitotically cycling population of germline stem cells is present at the distal end of the gonad. Successive temporal stages of differentiating germ cells are present consecutively in the distal-to-proximal axis with the fully-developed gametes at the proximal end. Sperm nuclei appear as tiny dots within the marked area. Whereas the tumor is on the distal side of sperm in the gld-1 single mutant, it is on the proximal side of sperm in the gld-1; puf-8 double mutant.

Mentions: Germline tumor in these worms was the gld-1-type. See Results and Figure 3 for more details. See Table S1 for the complete description of the genotypes, which includes the marker mutations.


PUF-8 Functions Redundantly with GLD-1 to Promote the Meiotic Progression of Spermatocytes in Caenorhabditis elegans.

Priti A, Subramaniam K - G3 (Bethesda) (2015)

Comparison of germ cell tumors between gld-1(-) and gld-1(-);puf-8(-) hermaphrodite germlines. Hermaphrodite gonads of the indicated genotypes stained with DAPI. Mitotically cycling population of germline stem cells is present at the distal end of the gonad. Successive temporal stages of differentiating germ cells are present consecutively in the distal-to-proximal axis with the fully-developed gametes at the proximal end. Sperm nuclei appear as tiny dots within the marked area. Whereas the tumor is on the distal side of sperm in the gld-1 single mutant, it is on the proximal side of sperm in the gld-1; puf-8 double mutant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4528324&req=5

fig3: Comparison of germ cell tumors between gld-1(-) and gld-1(-);puf-8(-) hermaphrodite germlines. Hermaphrodite gonads of the indicated genotypes stained with DAPI. Mitotically cycling population of germline stem cells is present at the distal end of the gonad. Successive temporal stages of differentiating germ cells are present consecutively in the distal-to-proximal axis with the fully-developed gametes at the proximal end. Sperm nuclei appear as tiny dots within the marked area. Whereas the tumor is on the distal side of sperm in the gld-1 single mutant, it is on the proximal side of sperm in the gld-1; puf-8 double mutant.
Mentions: Germline tumor in these worms was the gld-1-type. See Results and Figure 3 for more details. See Table S1 for the complete description of the genotypes, which includes the marker mutations.

Bottom Line: During larval development, gld-1; puf-8 double-mutant germ cells begin to express the meiotic marker HIM-3, lose P granules, and form the sperm-specific membranous organelle, which are characteristics of developing spermatocytes.However, some of these cells quickly lose HIM-3 and form germ cell tumors that lack membranous organelle but contain P granules.Together, results presented here uncover a novel function for gld-1 in the meiotic development of spermatocytes in both hermaphrodites and males.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences & Bioengineering, Indian Institute of Technology - Kanpur, Kanpur 208016, India.

No MeSH data available.


Related in: MedlinePlus