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PUF-8 Functions Redundantly with GLD-1 to Promote the Meiotic Progression of Spermatocytes in Caenorhabditis elegans.

Priti A, Subramaniam K - G3 (Bethesda) (2015)

Bottom Line: During larval development, gld-1; puf-8 double-mutant germ cells begin to express the meiotic marker HIM-3, lose P granules, and form the sperm-specific membranous organelle, which are characteristics of developing spermatocytes.However, some of these cells quickly lose HIM-3 and form germ cell tumors that lack membranous organelle but contain P granules.Together, results presented here uncover a novel function for gld-1 in the meiotic development of spermatocytes in both hermaphrodites and males.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences & Bioengineering, Indian Institute of Technology - Kanpur, Kanpur 208016, India.

No MeSH data available.


Related in: MedlinePlus

Specification of the male sexual fate of germ cells does not depend on gld-1 or puf-8. (A) Germlines of males, of the indicated genotypes and growth temperatures, stained with anti-membranous organelle (MO) antibodies (green) and DAPI (red). Cells positive for the sperm marker MO—outlined and marked sperm—are present on the distal side of the tumor in both gld-1(-) and gld-1(-); puf-8(-) germlines. (B) Male germlines stained for the oocyte marker RME-2 (green) and DAPI (red). No RME-2−positive cells are seen in any of the three genotypes shown. For a positive control of RME-2 immunostaining, see Figure 4B.
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fig2: Specification of the male sexual fate of germ cells does not depend on gld-1 or puf-8. (A) Germlines of males, of the indicated genotypes and growth temperatures, stained with anti-membranous organelle (MO) antibodies (green) and DAPI (red). Cells positive for the sperm marker MO—outlined and marked sperm—are present on the distal side of the tumor in both gld-1(-) and gld-1(-); puf-8(-) germlines. (B) Male germlines stained for the oocyte marker RME-2 (green) and DAPI (red). No RME-2−positive cells are seen in any of the three genotypes shown. For a positive control of RME-2 immunostaining, see Figure 4B.

Mentions: One possibility is that the germ cells of gld-1(-) males grown at 25° and gld-1(-); puf-8(-) males grown at 20° fail to enter spermatogenesis and instead switch to the oogenic mode. Female germ cells thus formed in the male germline probably exit meiosis and form tumors, like their counterparts in gld-1(-) hermaphrodites (Francis et al. 1995b). To test this possibility, we examined the germlines of these males for the presence of RME-2, an oocyte-specific yolk receptor, and the sperm-specific MO by immunostaining with specific antibodies (Ward et al. 1986; Grant and Hirsh 1999). Both gld-1(-) and gld-1(-); puf-8(-) male germlines did not express RME-2, but stained positively for MO (Figure 2), which shows that the germ cells did not switch to oogenic mode in these germlines. rme-2 mRNA is a well-known target of GLD-1, and RME-2 has been shown to be misexpressed in the pachytene region of gld-1(-) hermaphrodite germlines (Lee and Schedl 2001). However, the absence of RME-2 in male germlines missing GLD-1 is not surprising because the suppression of RME-2 expression in male germlines has been shown to be independent of GLD-1 (Ciosk et al. 2004). Together, the aforementioned data show that puf-8 and gld-1 function in a redundant fashion to suppress tumorigenesis in the male germline.


PUF-8 Functions Redundantly with GLD-1 to Promote the Meiotic Progression of Spermatocytes in Caenorhabditis elegans.

Priti A, Subramaniam K - G3 (Bethesda) (2015)

Specification of the male sexual fate of germ cells does not depend on gld-1 or puf-8. (A) Germlines of males, of the indicated genotypes and growth temperatures, stained with anti-membranous organelle (MO) antibodies (green) and DAPI (red). Cells positive for the sperm marker MO—outlined and marked sperm—are present on the distal side of the tumor in both gld-1(-) and gld-1(-); puf-8(-) germlines. (B) Male germlines stained for the oocyte marker RME-2 (green) and DAPI (red). No RME-2−positive cells are seen in any of the three genotypes shown. For a positive control of RME-2 immunostaining, see Figure 4B.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4528324&req=5

fig2: Specification of the male sexual fate of germ cells does not depend on gld-1 or puf-8. (A) Germlines of males, of the indicated genotypes and growth temperatures, stained with anti-membranous organelle (MO) antibodies (green) and DAPI (red). Cells positive for the sperm marker MO—outlined and marked sperm—are present on the distal side of the tumor in both gld-1(-) and gld-1(-); puf-8(-) germlines. (B) Male germlines stained for the oocyte marker RME-2 (green) and DAPI (red). No RME-2−positive cells are seen in any of the three genotypes shown. For a positive control of RME-2 immunostaining, see Figure 4B.
Mentions: One possibility is that the germ cells of gld-1(-) males grown at 25° and gld-1(-); puf-8(-) males grown at 20° fail to enter spermatogenesis and instead switch to the oogenic mode. Female germ cells thus formed in the male germline probably exit meiosis and form tumors, like their counterparts in gld-1(-) hermaphrodites (Francis et al. 1995b). To test this possibility, we examined the germlines of these males for the presence of RME-2, an oocyte-specific yolk receptor, and the sperm-specific MO by immunostaining with specific antibodies (Ward et al. 1986; Grant and Hirsh 1999). Both gld-1(-) and gld-1(-); puf-8(-) male germlines did not express RME-2, but stained positively for MO (Figure 2), which shows that the germ cells did not switch to oogenic mode in these germlines. rme-2 mRNA is a well-known target of GLD-1, and RME-2 has been shown to be misexpressed in the pachytene region of gld-1(-) hermaphrodite germlines (Lee and Schedl 2001). However, the absence of RME-2 in male germlines missing GLD-1 is not surprising because the suppression of RME-2 expression in male germlines has been shown to be independent of GLD-1 (Ciosk et al. 2004). Together, the aforementioned data show that puf-8 and gld-1 function in a redundant fashion to suppress tumorigenesis in the male germline.

Bottom Line: During larval development, gld-1; puf-8 double-mutant germ cells begin to express the meiotic marker HIM-3, lose P granules, and form the sperm-specific membranous organelle, which are characteristics of developing spermatocytes.However, some of these cells quickly lose HIM-3 and form germ cell tumors that lack membranous organelle but contain P granules.Together, results presented here uncover a novel function for gld-1 in the meiotic development of spermatocytes in both hermaphrodites and males.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences & Bioengineering, Indian Institute of Technology - Kanpur, Kanpur 208016, India.

No MeSH data available.


Related in: MedlinePlus