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An ex vivo model using human osteoarthritic cartilage demonstrates the release of bioactive insulin-like growth factor-1 from a collagen-glycosaminoglycan scaffold.

Wardale J, Mullen L, Howard D, Ghose S, Rushton N - Cell Biochem. Funct. (2015)

Bottom Line: Ex vivo models were stable throughout experiments, and cells remained viable.Chondrocytes seeded into the EVM attached throughout the scaffold and in contact with the cartilage explants.Cell migration and deposition of extracellular matrix proteins in the scaffold was enhanced by growth factors particularly if the scaffold was preloaded with growth factors.

View Article: PubMed Central - PubMed

Affiliation: Orthopaedic Research Unit, University of Cambridge, Cambridge, UK.

No MeSH data available.


Related in: MedlinePlus

Decorin deposition within the scaffold. (Top) Representative Western blot demonstrating the amount of decorin deposited by osteoarthritis chondrocytes within each scaffold group. Concentration of growth factors as in Table 1. Standard = extract of whole human articular cartilage. (Bottom) Decorin deposition in scaffolds quantified by densitometry of Western blot (top) expressed as fold change compared with the no-growth factor control group. N = 3 donors, N = 3 replicates. * represents a statistically significant difference between groups (p < 0.05) compared with control. IGF, insulin-like growth factor; TGF, transforming growth factor
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fig03: Decorin deposition within the scaffold. (Top) Representative Western blot demonstrating the amount of decorin deposited by osteoarthritis chondrocytes within each scaffold group. Concentration of growth factors as in Table 1. Standard = extract of whole human articular cartilage. (Bottom) Decorin deposition in scaffolds quantified by densitometry of Western blot (top) expressed as fold change compared with the no-growth factor control group. N = 3 donors, N = 3 replicates. * represents a statistically significant difference between groups (p < 0.05) compared with control. IGF, insulin-like growth factor; TGF, transforming growth factor

Mentions: Decorin was detected in all scaffold groups as shown in a representative Western blot [Figure3(top)]. However, when combined with analysis of replicate experiments using OA chondrocytes derived from other patients (not shown), only the AI group yielded statistically significant (p < 0.05) higher amounts of decorin deposition in the scaffold when compared with the control and the other GF groups [Figure3(bottom)].


An ex vivo model using human osteoarthritic cartilage demonstrates the release of bioactive insulin-like growth factor-1 from a collagen-glycosaminoglycan scaffold.

Wardale J, Mullen L, Howard D, Ghose S, Rushton N - Cell Biochem. Funct. (2015)

Decorin deposition within the scaffold. (Top) Representative Western blot demonstrating the amount of decorin deposited by osteoarthritis chondrocytes within each scaffold group. Concentration of growth factors as in Table 1. Standard = extract of whole human articular cartilage. (Bottom) Decorin deposition in scaffolds quantified by densitometry of Western blot (top) expressed as fold change compared with the no-growth factor control group. N = 3 donors, N = 3 replicates. * represents a statistically significant difference between groups (p < 0.05) compared with control. IGF, insulin-like growth factor; TGF, transforming growth factor
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4528234&req=5

fig03: Decorin deposition within the scaffold. (Top) Representative Western blot demonstrating the amount of decorin deposited by osteoarthritis chondrocytes within each scaffold group. Concentration of growth factors as in Table 1. Standard = extract of whole human articular cartilage. (Bottom) Decorin deposition in scaffolds quantified by densitometry of Western blot (top) expressed as fold change compared with the no-growth factor control group. N = 3 donors, N = 3 replicates. * represents a statistically significant difference between groups (p < 0.05) compared with control. IGF, insulin-like growth factor; TGF, transforming growth factor
Mentions: Decorin was detected in all scaffold groups as shown in a representative Western blot [Figure3(top)]. However, when combined with analysis of replicate experiments using OA chondrocytes derived from other patients (not shown), only the AI group yielded statistically significant (p < 0.05) higher amounts of decorin deposition in the scaffold when compared with the control and the other GF groups [Figure3(bottom)].

Bottom Line: Ex vivo models were stable throughout experiments, and cells remained viable.Chondrocytes seeded into the EVM attached throughout the scaffold and in contact with the cartilage explants.Cell migration and deposition of extracellular matrix proteins in the scaffold was enhanced by growth factors particularly if the scaffold was preloaded with growth factors.

View Article: PubMed Central - PubMed

Affiliation: Orthopaedic Research Unit, University of Cambridge, Cambridge, UK.

No MeSH data available.


Related in: MedlinePlus