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An ex vivo model using human osteoarthritic cartilage demonstrates the release of bioactive insulin-like growth factor-1 from a collagen-glycosaminoglycan scaffold.

Wardale J, Mullen L, Howard D, Ghose S, Rushton N - Cell Biochem. Funct. (2015)

Bottom Line: Ex vivo models were stable throughout experiments, and cells remained viable.Chondrocytes seeded into the EVM attached throughout the scaffold and in contact with the cartilage explants.Cell migration and deposition of extracellular matrix proteins in the scaffold was enhanced by growth factors particularly if the scaffold was preloaded with growth factors.

View Article: PubMed Central - PubMed

Affiliation: Orthopaedic Research Unit, University of Cambridge, Cambridge, UK.

No MeSH data available.


Related in: MedlinePlus

Characteristics of human articular cartilage ex vivo model (EVM). (A) EVM construction. (B) EVMs in 48-well plate. (C) EVM after 14 days in vitro. (D) EVM stained with safranin O. (E) and (F) EVM stained with toluidine blue after 14 days in vitro showing cells added at day 0 populating the scaffold (E) and in contact with the cartilage (F). Scale bar = 100 µm. OA, osteoarthritis
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fig01: Characteristics of human articular cartilage ex vivo model (EVM). (A) EVM construction. (B) EVMs in 48-well plate. (C) EVM after 14 days in vitro. (D) EVM stained with safranin O. (E) and (F) EVM stained with toluidine blue after 14 days in vitro showing cells added at day 0 populating the scaffold (E) and in contact with the cartilage (F). Scale bar = 100 µm. OA, osteoarthritis

Mentions: Incubated in media were 3 mm CG scaffold discs [Dulbecco's modified eagle medium containing 10% foetal calf serum, 100 IU/100 µg/ml penicillin/streptomycin and 20μg/ml ascorbate-2-phosphate (Sigma) containing IGF-1 (25 µg/ml), TGF-β1 (10 µg/ml), IGF-1 plus TGF-β1 or media only for 24 h at 37 °C]. Unbound GF was removed, and each scaffold seeded with 1 × 105 primary OA chondrocytes as described previously8 before insertion into each explant ring (Figure1A). EVM were cultured in 0.5 ml of complete media or complete media supplemented with exogenous growth factors for 28 days. Media were collected every 3–4 days and replaced with fresh media and fresh exogenous growth factors if applicable. Table 1 lists the experimental groups investigated.


An ex vivo model using human osteoarthritic cartilage demonstrates the release of bioactive insulin-like growth factor-1 from a collagen-glycosaminoglycan scaffold.

Wardale J, Mullen L, Howard D, Ghose S, Rushton N - Cell Biochem. Funct. (2015)

Characteristics of human articular cartilage ex vivo model (EVM). (A) EVM construction. (B) EVMs in 48-well plate. (C) EVM after 14 days in vitro. (D) EVM stained with safranin O. (E) and (F) EVM stained with toluidine blue after 14 days in vitro showing cells added at day 0 populating the scaffold (E) and in contact with the cartilage (F). Scale bar = 100 µm. OA, osteoarthritis
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4528234&req=5

fig01: Characteristics of human articular cartilage ex vivo model (EVM). (A) EVM construction. (B) EVMs in 48-well plate. (C) EVM after 14 days in vitro. (D) EVM stained with safranin O. (E) and (F) EVM stained with toluidine blue after 14 days in vitro showing cells added at day 0 populating the scaffold (E) and in contact with the cartilage (F). Scale bar = 100 µm. OA, osteoarthritis
Mentions: Incubated in media were 3 mm CG scaffold discs [Dulbecco's modified eagle medium containing 10% foetal calf serum, 100 IU/100 µg/ml penicillin/streptomycin and 20μg/ml ascorbate-2-phosphate (Sigma) containing IGF-1 (25 µg/ml), TGF-β1 (10 µg/ml), IGF-1 plus TGF-β1 or media only for 24 h at 37 °C]. Unbound GF was removed, and each scaffold seeded with 1 × 105 primary OA chondrocytes as described previously8 before insertion into each explant ring (Figure1A). EVM were cultured in 0.5 ml of complete media or complete media supplemented with exogenous growth factors for 28 days. Media were collected every 3–4 days and replaced with fresh media and fresh exogenous growth factors if applicable. Table 1 lists the experimental groups investigated.

Bottom Line: Ex vivo models were stable throughout experiments, and cells remained viable.Chondrocytes seeded into the EVM attached throughout the scaffold and in contact with the cartilage explants.Cell migration and deposition of extracellular matrix proteins in the scaffold was enhanced by growth factors particularly if the scaffold was preloaded with growth factors.

View Article: PubMed Central - PubMed

Affiliation: Orthopaedic Research Unit, University of Cambridge, Cambridge, UK.

No MeSH data available.


Related in: MedlinePlus