Cellular basis of neuroepithelial bending during mouse spinal neural tube closure.
Bottom Line: As the neural folds elevate, cell numbers increase to a greater extent in the dorsolateral neural plate that contacts the surface ectoderm, compared with the more ventromedial neural plate where cells contact paraxial mesoderm and notochord.We hypothesised that neuroepithelial cells may translocate in a ventral-to-dorsal direction as DLHP formation occurs, and this was confirmed by vital cell labelling in cultured embryos.These findings suggest a model in which DLHP formation may proceed through 'buckling' of the neuroepithelium at a dorso-ventral boundary marked by a change in cell-packing density.
Affiliation: Newlife Birth Defects Research Centre, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK.Show MeSH
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Mentions: Neural plate cell (nuclear) number increased from flat to elevated neuroepithelium mainly in Modes 2 and 3 of neurulation (Fig. 3A-C). Moreover, this cell number increase was most dramatic within the neural fold, where the transition from flat to elevated neural plate was marked by a 5-fold increase in cell number at Mode 3, compared with only a 2-fold increase at Mode 1 (Fig. 3C). In contrast, much smaller cell number increases occurred during elevation outside the neural fold, and cell number did not differ significantly in this region between the elevated neural plate of Modes 1, 2 and 3 (Fig. 3B).
Affiliation: Newlife Birth Defects Research Centre, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK.