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Cellular basis of neuroepithelial bending during mouse spinal neural tube closure.

McShane SG, Molè MA, Savery D, Greene ND, Tam PP, Copp AJ - Dev. Biol. (2015)

Bottom Line: As the neural folds elevate, cell numbers increase to a greater extent in the dorsolateral neural plate that contacts the surface ectoderm, compared with the more ventromedial neural plate where cells contact paraxial mesoderm and notochord.The translocation of cells into the neural fold, together with its more rapid cell proliferation, leads to an increase in cell density dorsolaterally compared with the more ventromedial neural plate.These findings suggest a model in which DLHP formation may proceed through 'buckling' of the neuroepithelium at a dorso-ventral boundary marked by a change in cell-packing density.

View Article: PubMed Central - PubMed

Affiliation: Newlife Birth Defects Research Centre, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK.

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Cell number in neural plate (NP) and juxtaposed surface ectoderm (SE) during neural plate bending. (A-D) Mean cell number in the entire half-NP (A), in NP sub-domains outside (B) and within (C) the neural fold, and in SE juxtaposed to the NP (D). Elevated NP and SE (grey bars) contain significantly more cells than flat NP and SE (green bars) in most comparisons (*p<0.001). Cell number of elevated NP in the neural fold (C) and juxtaposed SE (D) increase significantly from Mode 1 to Mode 2, and from Mode 2 to Mode 3 (**p<0.001). Cell number in total elevated NP (A) also increases significantly from Mode 1 to Modes 2 and 3 (**p=0.014). In contrast, cell number in NP outside the neural fold (B) varies with neurulation mode only for flat NP (**p=0.006). (E) NP/SE cell number ratio within neural fold increases significantly from flat to elevated neural plate (*p<0.001). Within the elevated neural fold, NP/SE ratio is significantly greater in Modes 2 and 3, which have DLHPs, than in Mode 1 which lacks DLHPs (**p=0.007).
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f0015: Cell number in neural plate (NP) and juxtaposed surface ectoderm (SE) during neural plate bending. (A-D) Mean cell number in the entire half-NP (A), in NP sub-domains outside (B) and within (C) the neural fold, and in SE juxtaposed to the NP (D). Elevated NP and SE (grey bars) contain significantly more cells than flat NP and SE (green bars) in most comparisons (*p<0.001). Cell number of elevated NP in the neural fold (C) and juxtaposed SE (D) increase significantly from Mode 1 to Mode 2, and from Mode 2 to Mode 3 (**p<0.001). Cell number in total elevated NP (A) also increases significantly from Mode 1 to Modes 2 and 3 (**p=0.014). In contrast, cell number in NP outside the neural fold (B) varies with neurulation mode only for flat NP (**p=0.006). (E) NP/SE cell number ratio within neural fold increases significantly from flat to elevated neural plate (*p<0.001). Within the elevated neural fold, NP/SE ratio is significantly greater in Modes 2 and 3, which have DLHPs, than in Mode 1 which lacks DLHPs (**p=0.007).

Mentions: Neural plate cell (nuclear) number increased from flat to elevated neuroepithelium mainly in Modes 2 and 3 of neurulation (Fig. 3A-C). Moreover, this cell number increase was most dramatic within the neural fold, where the transition from flat to elevated neural plate was marked by a 5-fold increase in cell number at Mode 3, compared with only a 2-fold increase at Mode 1 (Fig. 3C). In contrast, much smaller cell number increases occurred during elevation outside the neural fold, and cell number did not differ significantly in this region between the elevated neural plate of Modes 1, 2 and 3 (Fig. 3B).


Cellular basis of neuroepithelial bending during mouse spinal neural tube closure.

McShane SG, Molè MA, Savery D, Greene ND, Tam PP, Copp AJ - Dev. Biol. (2015)

Cell number in neural plate (NP) and juxtaposed surface ectoderm (SE) during neural plate bending. (A-D) Mean cell number in the entire half-NP (A), in NP sub-domains outside (B) and within (C) the neural fold, and in SE juxtaposed to the NP (D). Elevated NP and SE (grey bars) contain significantly more cells than flat NP and SE (green bars) in most comparisons (*p<0.001). Cell number of elevated NP in the neural fold (C) and juxtaposed SE (D) increase significantly from Mode 1 to Mode 2, and from Mode 2 to Mode 3 (**p<0.001). Cell number in total elevated NP (A) also increases significantly from Mode 1 to Modes 2 and 3 (**p=0.014). In contrast, cell number in NP outside the neural fold (B) varies with neurulation mode only for flat NP (**p=0.006). (E) NP/SE cell number ratio within neural fold increases significantly from flat to elevated neural plate (*p<0.001). Within the elevated neural fold, NP/SE ratio is significantly greater in Modes 2 and 3, which have DLHPs, than in Mode 1 which lacks DLHPs (**p=0.007).
© Copyright Policy - CC BY
Related In: Results  -  Collection

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f0015: Cell number in neural plate (NP) and juxtaposed surface ectoderm (SE) during neural plate bending. (A-D) Mean cell number in the entire half-NP (A), in NP sub-domains outside (B) and within (C) the neural fold, and in SE juxtaposed to the NP (D). Elevated NP and SE (grey bars) contain significantly more cells than flat NP and SE (green bars) in most comparisons (*p<0.001). Cell number of elevated NP in the neural fold (C) and juxtaposed SE (D) increase significantly from Mode 1 to Mode 2, and from Mode 2 to Mode 3 (**p<0.001). Cell number in total elevated NP (A) also increases significantly from Mode 1 to Modes 2 and 3 (**p=0.014). In contrast, cell number in NP outside the neural fold (B) varies with neurulation mode only for flat NP (**p=0.006). (E) NP/SE cell number ratio within neural fold increases significantly from flat to elevated neural plate (*p<0.001). Within the elevated neural fold, NP/SE ratio is significantly greater in Modes 2 and 3, which have DLHPs, than in Mode 1 which lacks DLHPs (**p=0.007).
Mentions: Neural plate cell (nuclear) number increased from flat to elevated neuroepithelium mainly in Modes 2 and 3 of neurulation (Fig. 3A-C). Moreover, this cell number increase was most dramatic within the neural fold, where the transition from flat to elevated neural plate was marked by a 5-fold increase in cell number at Mode 3, compared with only a 2-fold increase at Mode 1 (Fig. 3C). In contrast, much smaller cell number increases occurred during elevation outside the neural fold, and cell number did not differ significantly in this region between the elevated neural plate of Modes 1, 2 and 3 (Fig. 3B).

Bottom Line: As the neural folds elevate, cell numbers increase to a greater extent in the dorsolateral neural plate that contacts the surface ectoderm, compared with the more ventromedial neural plate where cells contact paraxial mesoderm and notochord.The translocation of cells into the neural fold, together with its more rapid cell proliferation, leads to an increase in cell density dorsolaterally compared with the more ventromedial neural plate.These findings suggest a model in which DLHP formation may proceed through 'buckling' of the neuroepithelium at a dorso-ventral boundary marked by a change in cell-packing density.

View Article: PubMed Central - PubMed

Affiliation: Newlife Birth Defects Research Centre, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK.

Show MeSH