Cellular basis of neuroepithelial bending during mouse spinal neural tube closure.
Bottom Line: As the neural folds elevate, cell numbers increase to a greater extent in the dorsolateral neural plate that contacts the surface ectoderm, compared with the more ventromedial neural plate where cells contact paraxial mesoderm and notochord.We hypothesised that neuroepithelial cells may translocate in a ventral-to-dorsal direction as DLHP formation occurs, and this was confirmed by vital cell labelling in cultured embryos.These findings suggest a model in which DLHP formation may proceed through 'buckling' of the neuroepithelium at a dorso-ventral boundary marked by a change in cell-packing density.
Affiliation: Newlife Birth Defects Research Centre, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK.Show MeSH
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Mentions: Embryos within specific somite number ranges were selected for analysis, in order to represent the characteristic morphology of the three Modes of neurulation: Mode 1 (MHP only), 10-12 somites; Mode 2 (MHP and DLHPs), 19-21 somites; Mode 3 (DLHPs only), 28-30 somites. Five embryos of each Mode were analysed with data gathered in each embryo from two rostro-caudal levels of the posterior neuropore (PNP): flat and elevated neural plate (Fig. 1).
Affiliation: Newlife Birth Defects Research Centre, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK.