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Anti-inflammatory activity of Nerium indicum by inhibition of prostaglandin E2 in murine splenic lymphocytes.

Dey P, Chaudhuri TK - Indian J Pharmacol (2015 Jul-Aug)

Bottom Line: The results demonstrated a 2.26-fold inhibition of PGE2 level at 80 μg/mL of NILE.Linear correlation analysis of the dose-dependent PGE₂ inhibition with other pro- and anti-inflammatory mediators demonstrated high inter-correlation between the parameters.Thus, the present study remains in accordance with our previous report and confirms the anti-inflammatory claim of N. indicum, mentioned in the traditional medicine.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Cellular Immunology Laboratory, University of North Bengal, Siliguri, West Bengal, India.

ABSTRACT

Objective: Nerium indicum Mill (syn. N. oleander L. and N. odorum Aiton; family: Apocynaceae) is a medicinal plant, used in the treatment of diverse ailments including various chronic inflammatory diseases in traditional medicine. We have previously demonstrated the immunomodulatory activity of a bioactive fraction of Nerium indicum leaf (NILE) by studying up-regulation of interleukin-2 (IL-2), IL-10, interferon-gamma and down regulation of IL-4, tumor necrosis factor-alpha (TNF-α), nitric oxide, cyclooxygenase-1 (COX-1) and COX-2 activities. Therefore, this study aimed to confirm the anti-inflammatory activity of NILE by inhibition of prostaglandin E₂ (PGE₂) activity in murine splenic lymphocytes in vitro.

Materials and methods: Murine lymphocytes were isolated from spleen and stimulated with 5 ΅g/mL concanavalin A in RPMI-1640, supplemented with 50 U/mL penicillin, 50 U/mL streptomycin, 50 U/mL nystatin and 10% fetal bovine serum. Different concentrations (0-80 μg/mL) of NILE were added and the cells were cultured for 48 h. The culture supernatants were thereafter collected by centrifugation and assayed for expression of PGE₂ level. The data were analyzed statistically.

Results: The results demonstrated a 2.26-fold inhibition of PGE2 level at 80 μg/mL of NILE. Half maximum inhibitory concentration (IC₅₀) was calculated to be 44.95 ± 0.45 ΅g/mL. Linear correlation analysis of the dose-dependent PGE₂ inhibition with other pro- and anti-inflammatory mediators demonstrated high inter-correlation between the parameters.

Conclusions: Thus, the present study remains in accordance with our previous report and confirms the anti-inflammatory claim of N. indicum, mentioned in the traditional medicine.

No MeSH data available.


Related in: MedlinePlus

Correlation of prostaglandin E2 level with the expression of different pro- and anti-inflammatory mediators. Axes “x” and “y” denotes correlation points of respective parameters measured at pg/mL at different dose of Nerium indicum leaf (0–80 μg/mL). Where, R2 = coefficient of determination
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Figure 3: Correlation of prostaglandin E2 level with the expression of different pro- and anti-inflammatory mediators. Axes “x” and “y” denotes correlation points of respective parameters measured at pg/mL at different dose of Nerium indicum leaf (0–80 μg/mL). Where, R2 = coefficient of determination

Mentions: The linear correlation analysis between the PGE2 level with different pro- and anti-inflammatory signals are demonstrated in Figure 3. High positive correlation between PGE2 level with COX-1 (R2 =0.6238) and COX-2 activities (R2 =0.6014). The highest degree of correlation resided between PGE2 and IL-2 level (R2 =0.895).


Anti-inflammatory activity of Nerium indicum by inhibition of prostaglandin E2 in murine splenic lymphocytes.

Dey P, Chaudhuri TK - Indian J Pharmacol (2015 Jul-Aug)

Correlation of prostaglandin E2 level with the expression of different pro- and anti-inflammatory mediators. Axes “x” and “y” denotes correlation points of respective parameters measured at pg/mL at different dose of Nerium indicum leaf (0–80 μg/mL). Where, R2 = coefficient of determination
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4527071&req=5

Figure 3: Correlation of prostaglandin E2 level with the expression of different pro- and anti-inflammatory mediators. Axes “x” and “y” denotes correlation points of respective parameters measured at pg/mL at different dose of Nerium indicum leaf (0–80 μg/mL). Where, R2 = coefficient of determination
Mentions: The linear correlation analysis between the PGE2 level with different pro- and anti-inflammatory signals are demonstrated in Figure 3. High positive correlation between PGE2 level with COX-1 (R2 =0.6238) and COX-2 activities (R2 =0.6014). The highest degree of correlation resided between PGE2 and IL-2 level (R2 =0.895).

Bottom Line: The results demonstrated a 2.26-fold inhibition of PGE2 level at 80 μg/mL of NILE.Linear correlation analysis of the dose-dependent PGE₂ inhibition with other pro- and anti-inflammatory mediators demonstrated high inter-correlation between the parameters.Thus, the present study remains in accordance with our previous report and confirms the anti-inflammatory claim of N. indicum, mentioned in the traditional medicine.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Cellular Immunology Laboratory, University of North Bengal, Siliguri, West Bengal, India.

ABSTRACT

Objective: Nerium indicum Mill (syn. N. oleander L. and N. odorum Aiton; family: Apocynaceae) is a medicinal plant, used in the treatment of diverse ailments including various chronic inflammatory diseases in traditional medicine. We have previously demonstrated the immunomodulatory activity of a bioactive fraction of Nerium indicum leaf (NILE) by studying up-regulation of interleukin-2 (IL-2), IL-10, interferon-gamma and down regulation of IL-4, tumor necrosis factor-alpha (TNF-α), nitric oxide, cyclooxygenase-1 (COX-1) and COX-2 activities. Therefore, this study aimed to confirm the anti-inflammatory activity of NILE by inhibition of prostaglandin E₂ (PGE₂) activity in murine splenic lymphocytes in vitro.

Materials and methods: Murine lymphocytes were isolated from spleen and stimulated with 5 ΅g/mL concanavalin A in RPMI-1640, supplemented with 50 U/mL penicillin, 50 U/mL streptomycin, 50 U/mL nystatin and 10% fetal bovine serum. Different concentrations (0-80 μg/mL) of NILE were added and the cells were cultured for 48 h. The culture supernatants were thereafter collected by centrifugation and assayed for expression of PGE₂ level. The data were analyzed statistically.

Results: The results demonstrated a 2.26-fold inhibition of PGE2 level at 80 μg/mL of NILE. Half maximum inhibitory concentration (IC₅₀) was calculated to be 44.95 ± 0.45 ΅g/mL. Linear correlation analysis of the dose-dependent PGE₂ inhibition with other pro- and anti-inflammatory mediators demonstrated high inter-correlation between the parameters.

Conclusions: Thus, the present study remains in accordance with our previous report and confirms the anti-inflammatory claim of N. indicum, mentioned in the traditional medicine.

No MeSH data available.


Related in: MedlinePlus