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Anti-inflammatory activity of Nerium indicum by inhibition of prostaglandin E2 in murine splenic lymphocytes.

Dey P, Chaudhuri TK - Indian J Pharmacol (2015 Jul-Aug)

Bottom Line: The results demonstrated a 2.26-fold inhibition of PGE2 level at 80 μg/mL of NILE.Linear correlation analysis of the dose-dependent PGE₂ inhibition with other pro- and anti-inflammatory mediators demonstrated high inter-correlation between the parameters.Thus, the present study remains in accordance with our previous report and confirms the anti-inflammatory claim of N. indicum, mentioned in the traditional medicine.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Cellular Immunology Laboratory, University of North Bengal, Siliguri, West Bengal, India.

ABSTRACT

Objective: Nerium indicum Mill (syn. N. oleander L. and N. odorum Aiton; family: Apocynaceae) is a medicinal plant, used in the treatment of diverse ailments including various chronic inflammatory diseases in traditional medicine. We have previously demonstrated the immunomodulatory activity of a bioactive fraction of Nerium indicum leaf (NILE) by studying up-regulation of interleukin-2 (IL-2), IL-10, interferon-gamma and down regulation of IL-4, tumor necrosis factor-alpha (TNF-α), nitric oxide, cyclooxygenase-1 (COX-1) and COX-2 activities. Therefore, this study aimed to confirm the anti-inflammatory activity of NILE by inhibition of prostaglandin E₂ (PGE₂) activity in murine splenic lymphocytes in vitro.

Materials and methods: Murine lymphocytes were isolated from spleen and stimulated with 5 ΅g/mL concanavalin A in RPMI-1640, supplemented with 50 U/mL penicillin, 50 U/mL streptomycin, 50 U/mL nystatin and 10% fetal bovine serum. Different concentrations (0-80 μg/mL) of NILE were added and the cells were cultured for 48 h. The culture supernatants were thereafter collected by centrifugation and assayed for expression of PGE₂ level. The data were analyzed statistically.

Results: The results demonstrated a 2.26-fold inhibition of PGE2 level at 80 μg/mL of NILE. Half maximum inhibitory concentration (IC₅₀) was calculated to be 44.95 ± 0.45 ΅g/mL. Linear correlation analysis of the dose-dependent PGE₂ inhibition with other pro- and anti-inflammatory mediators demonstrated high inter-correlation between the parameters.

Conclusions: Thus, the present study remains in accordance with our previous report and confirms the anti-inflammatory claim of N. indicum, mentioned in the traditional medicine.

No MeSH data available.


Related in: MedlinePlus

Percentage of inhibition of prostaglandin E2 under different doses (0–80 μg/mL) of Nerium indicum leaf. Data are represented as mean ± standard deviation of six observations. ***P < 0.001 compared to 0 μg/mL group
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Figure 2: Percentage of inhibition of prostaglandin E2 under different doses (0–80 μg/mL) of Nerium indicum leaf. Data are represented as mean ± standard deviation of six observations. ***P < 0.001 compared to 0 μg/mL group

Mentions: This study demonstrated that NILE has the potentiality to inhibit PGE2 synthesis in murine splenic lymphocytes [Figure 1]. PGE2 level was increased to 649.30 ± 44.6 pg/mL (4.6-fold increase compared to control) due to stimulation with Con A, which upon NILE treatment at 80 μg/mL, was significantly down-regulated (P < 0.001) to 286.06 ± 26.75 pg/mL (2.26-fold decrease compared to 0 μg/mL). The percentage of inhibition (inhibitory concentration was 44.95 ± 0.45 μg/mL) of PGE2 is presented in Figure 2. This demonstrated in vitro dose-dependent activity of NILE to inhibit PGE2 levels.


Anti-inflammatory activity of Nerium indicum by inhibition of prostaglandin E2 in murine splenic lymphocytes.

Dey P, Chaudhuri TK - Indian J Pharmacol (2015 Jul-Aug)

Percentage of inhibition of prostaglandin E2 under different doses (0–80 μg/mL) of Nerium indicum leaf. Data are represented as mean ± standard deviation of six observations. ***P < 0.001 compared to 0 μg/mL group
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4527071&req=5

Figure 2: Percentage of inhibition of prostaglandin E2 under different doses (0–80 μg/mL) of Nerium indicum leaf. Data are represented as mean ± standard deviation of six observations. ***P < 0.001 compared to 0 μg/mL group
Mentions: This study demonstrated that NILE has the potentiality to inhibit PGE2 synthesis in murine splenic lymphocytes [Figure 1]. PGE2 level was increased to 649.30 ± 44.6 pg/mL (4.6-fold increase compared to control) due to stimulation with Con A, which upon NILE treatment at 80 μg/mL, was significantly down-regulated (P < 0.001) to 286.06 ± 26.75 pg/mL (2.26-fold decrease compared to 0 μg/mL). The percentage of inhibition (inhibitory concentration was 44.95 ± 0.45 μg/mL) of PGE2 is presented in Figure 2. This demonstrated in vitro dose-dependent activity of NILE to inhibit PGE2 levels.

Bottom Line: The results demonstrated a 2.26-fold inhibition of PGE2 level at 80 μg/mL of NILE.Linear correlation analysis of the dose-dependent PGE₂ inhibition with other pro- and anti-inflammatory mediators demonstrated high inter-correlation between the parameters.Thus, the present study remains in accordance with our previous report and confirms the anti-inflammatory claim of N. indicum, mentioned in the traditional medicine.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Cellular Immunology Laboratory, University of North Bengal, Siliguri, West Bengal, India.

ABSTRACT

Objective: Nerium indicum Mill (syn. N. oleander L. and N. odorum Aiton; family: Apocynaceae) is a medicinal plant, used in the treatment of diverse ailments including various chronic inflammatory diseases in traditional medicine. We have previously demonstrated the immunomodulatory activity of a bioactive fraction of Nerium indicum leaf (NILE) by studying up-regulation of interleukin-2 (IL-2), IL-10, interferon-gamma and down regulation of IL-4, tumor necrosis factor-alpha (TNF-α), nitric oxide, cyclooxygenase-1 (COX-1) and COX-2 activities. Therefore, this study aimed to confirm the anti-inflammatory activity of NILE by inhibition of prostaglandin E₂ (PGE₂) activity in murine splenic lymphocytes in vitro.

Materials and methods: Murine lymphocytes were isolated from spleen and stimulated with 5 ΅g/mL concanavalin A in RPMI-1640, supplemented with 50 U/mL penicillin, 50 U/mL streptomycin, 50 U/mL nystatin and 10% fetal bovine serum. Different concentrations (0-80 μg/mL) of NILE were added and the cells were cultured for 48 h. The culture supernatants were thereafter collected by centrifugation and assayed for expression of PGE₂ level. The data were analyzed statistically.

Results: The results demonstrated a 2.26-fold inhibition of PGE2 level at 80 μg/mL of NILE. Half maximum inhibitory concentration (IC₅₀) was calculated to be 44.95 ± 0.45 ΅g/mL. Linear correlation analysis of the dose-dependent PGE₂ inhibition with other pro- and anti-inflammatory mediators demonstrated high inter-correlation between the parameters.

Conclusions: Thus, the present study remains in accordance with our previous report and confirms the anti-inflammatory claim of N. indicum, mentioned in the traditional medicine.

No MeSH data available.


Related in: MedlinePlus