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In-vitro α-amylase and α-glucosidase inhibitory activity of Adiantum caudatum Linn. and Celosia argentea Linn. extracts and fractions.

Telagari M, Hullatti K - Indian J Pharmacol (2015 Jul-Aug)

Bottom Line: Whereas, extracts and remaining fractions of both the plants have shown lesser activity.The results of the present study indicate that, fraction 2 of A. caudatum, rich in triterpenoids and phenolics and fraction 4 of C. argentea, rich in flavonoids, are effective α- amylase and α- glucosidase inhibitors, which may be helpful to reduce the postprandial glucose levels.Hence, further studies may throw light on the antidiabetic potential of A. caudatum and C. argentea, especially in the management of type 2 diabetes.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, KLES College of Pharmacy, Belagavi, Karnataka, India.

ABSTRACT

Objective: The objective of the present study was to provide an in-vitro evidence for the potential inhibitory activity of extracts and fractions of Adiantum caudatum Linn. and Celosia argentea Linn. on α-amylase and α-glucosidase enzymes.

Materials and methods: The plant extracts were prepared, first with cold maceration (70% v/v ethanol) and then by Soxhlation techniques (95% v/v ethanol). Subsequently, the combined extracts were subjected for fractionation. Different concentrations (0.1, 0.2, 0.3, 0.4, and 0.5 mg/ml) of extract and fractions were subjected to α-amylase and α-glucosidase inhibitory assay. The absorbance was measured at 540 and 405 nm using multiplate reader and the percentage of α- amylase and α- glucosidase inhibitory activity and IC₅₀ values of extract and fractions were calculated.

Results: Fraction 2 of A. caudatum and fraction 4 of C. argentea has shown highest α-amylase and α-glucosidase inhibitory potential with IC₅₀ values of 0.241, 0.211 and 0.294, 0.249 mg/ml, respectively, which was comparable with acarbose (0.125 and 0.93 mg/ml). Whereas, extracts and remaining fractions of both the plants have shown lesser activity.

Conclusion: The results of the present study indicate that, fraction 2 of A. caudatum, rich in triterpenoids and phenolics and fraction 4 of C. argentea, rich in flavonoids, are effective α- amylase and α- glucosidase inhibitors, which may be helpful to reduce the postprandial glucose levels. Hence, further studies may throw light on the antidiabetic potential of A. caudatum and C. argentea, especially in the management of type 2 diabetes.

No MeSH data available.


Related in: MedlinePlus

α-amylase inhibition of Adiantum caudatum extract and fractions
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Figure 2: α-amylase inhibition of Adiantum caudatum extract and fractions

Mentions: In the present study, hydro-alcoholic extract and four fractions of A. caudatum and C. argentea were evaluated for their inhibitory effect on α-amylase and α-glucosidase enzymes by in-vitro method. The hydro-alcoholic extract and its fractions 1, 2, 3, and 4 of A. caudatum (at a concentration of 0.5 mg/ml) exhibited 32.42, 46.25, 61.45, 20.12, and 26.04 α-amylase inhibitory activity [Figure 2] and 36.42, 47.25, 63.45, 29.22, and 32.34 α-glucosidase inhibitory activity [Figure 3], respectively. Whereas, C. argentea hydro-alcoholic extract and Fractions 1, 2, 3, and 4 exhibited 22.42, 21.04, 29.04, 30.12, and 59.45 α-amylase inhibitory activity [Figure 4], 37.62, 31.04, 37.24, 29.34, and 61.45 α-glucosidase inhibitory activity, respectively [Figure 5]. Acarbose was used as a standard reference drug, which showed α-amylase inhibitory activity with an IC50 value of 0.108 mg/ml and α-glucosidase inhibitory activity with an IC50 value of 0.083 mg/ml. Among all, fraction 2 of A. caudatum and fraction 4 of C. argentea has shown best enzyme inhibitory activity with an IC50 value 0.241 and 0.211 (α-amylase and α-glucosidase) [Table 1] and 0.294 and 0.249 mg/ml (α-amylase and α-glucosidase) [Table 2] which were comparable with that of acarbose.


In-vitro α-amylase and α-glucosidase inhibitory activity of Adiantum caudatum Linn. and Celosia argentea Linn. extracts and fractions.

Telagari M, Hullatti K - Indian J Pharmacol (2015 Jul-Aug)

α-amylase inhibition of Adiantum caudatum extract and fractions
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4527066&req=5

Figure 2: α-amylase inhibition of Adiantum caudatum extract and fractions
Mentions: In the present study, hydro-alcoholic extract and four fractions of A. caudatum and C. argentea were evaluated for their inhibitory effect on α-amylase and α-glucosidase enzymes by in-vitro method. The hydro-alcoholic extract and its fractions 1, 2, 3, and 4 of A. caudatum (at a concentration of 0.5 mg/ml) exhibited 32.42, 46.25, 61.45, 20.12, and 26.04 α-amylase inhibitory activity [Figure 2] and 36.42, 47.25, 63.45, 29.22, and 32.34 α-glucosidase inhibitory activity [Figure 3], respectively. Whereas, C. argentea hydro-alcoholic extract and Fractions 1, 2, 3, and 4 exhibited 22.42, 21.04, 29.04, 30.12, and 59.45 α-amylase inhibitory activity [Figure 4], 37.62, 31.04, 37.24, 29.34, and 61.45 α-glucosidase inhibitory activity, respectively [Figure 5]. Acarbose was used as a standard reference drug, which showed α-amylase inhibitory activity with an IC50 value of 0.108 mg/ml and α-glucosidase inhibitory activity with an IC50 value of 0.083 mg/ml. Among all, fraction 2 of A. caudatum and fraction 4 of C. argentea has shown best enzyme inhibitory activity with an IC50 value 0.241 and 0.211 (α-amylase and α-glucosidase) [Table 1] and 0.294 and 0.249 mg/ml (α-amylase and α-glucosidase) [Table 2] which were comparable with that of acarbose.

Bottom Line: Whereas, extracts and remaining fractions of both the plants have shown lesser activity.The results of the present study indicate that, fraction 2 of A. caudatum, rich in triterpenoids and phenolics and fraction 4 of C. argentea, rich in flavonoids, are effective α- amylase and α- glucosidase inhibitors, which may be helpful to reduce the postprandial glucose levels.Hence, further studies may throw light on the antidiabetic potential of A. caudatum and C. argentea, especially in the management of type 2 diabetes.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, KLES College of Pharmacy, Belagavi, Karnataka, India.

ABSTRACT

Objective: The objective of the present study was to provide an in-vitro evidence for the potential inhibitory activity of extracts and fractions of Adiantum caudatum Linn. and Celosia argentea Linn. on α-amylase and α-glucosidase enzymes.

Materials and methods: The plant extracts were prepared, first with cold maceration (70% v/v ethanol) and then by Soxhlation techniques (95% v/v ethanol). Subsequently, the combined extracts were subjected for fractionation. Different concentrations (0.1, 0.2, 0.3, 0.4, and 0.5 mg/ml) of extract and fractions were subjected to α-amylase and α-glucosidase inhibitory assay. The absorbance was measured at 540 and 405 nm using multiplate reader and the percentage of α- amylase and α- glucosidase inhibitory activity and IC₅₀ values of extract and fractions were calculated.

Results: Fraction 2 of A. caudatum and fraction 4 of C. argentea has shown highest α-amylase and α-glucosidase inhibitory potential with IC₅₀ values of 0.241, 0.211 and 0.294, 0.249 mg/ml, respectively, which was comparable with acarbose (0.125 and 0.93 mg/ml). Whereas, extracts and remaining fractions of both the plants have shown lesser activity.

Conclusion: The results of the present study indicate that, fraction 2 of A. caudatum, rich in triterpenoids and phenolics and fraction 4 of C. argentea, rich in flavonoids, are effective α- amylase and α- glucosidase inhibitors, which may be helpful to reduce the postprandial glucose levels. Hence, further studies may throw light on the antidiabetic potential of A. caudatum and C. argentea, especially in the management of type 2 diabetes.

No MeSH data available.


Related in: MedlinePlus