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In-vitro α-amylase and α-glucosidase inhibitory activity of Adiantum caudatum Linn. and Celosia argentea Linn. extracts and fractions.

Telagari M, Hullatti K - Indian J Pharmacol (2015 Jul-Aug)

Bottom Line: Whereas, extracts and remaining fractions of both the plants have shown lesser activity.The results of the present study indicate that, fraction 2 of A. caudatum, rich in triterpenoids and phenolics and fraction 4 of C. argentea, rich in flavonoids, are effective α- amylase and α- glucosidase inhibitors, which may be helpful to reduce the postprandial glucose levels.Hence, further studies may throw light on the antidiabetic potential of A. caudatum and C. argentea, especially in the management of type 2 diabetes.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, KLES College of Pharmacy, Belagavi, Karnataka, India.

ABSTRACT

Objective: The objective of the present study was to provide an in-vitro evidence for the potential inhibitory activity of extracts and fractions of Adiantum caudatum Linn. and Celosia argentea Linn. on α-amylase and α-glucosidase enzymes.

Materials and methods: The plant extracts were prepared, first with cold maceration (70% v/v ethanol) and then by Soxhlation techniques (95% v/v ethanol). Subsequently, the combined extracts were subjected for fractionation. Different concentrations (0.1, 0.2, 0.3, 0.4, and 0.5 mg/ml) of extract and fractions were subjected to α-amylase and α-glucosidase inhibitory assay. The absorbance was measured at 540 and 405 nm using multiplate reader and the percentage of α- amylase and α- glucosidase inhibitory activity and IC₅₀ values of extract and fractions were calculated.

Results: Fraction 2 of A. caudatum and fraction 4 of C. argentea has shown highest α-amylase and α-glucosidase inhibitory potential with IC₅₀ values of 0.241, 0.211 and 0.294, 0.249 mg/ml, respectively, which was comparable with acarbose (0.125 and 0.93 mg/ml). Whereas, extracts and remaining fractions of both the plants have shown lesser activity.

Conclusion: The results of the present study indicate that, fraction 2 of A. caudatum, rich in triterpenoids and phenolics and fraction 4 of C. argentea, rich in flavonoids, are effective α- amylase and α- glucosidase inhibitors, which may be helpful to reduce the postprandial glucose levels. Hence, further studies may throw light on the antidiabetic potential of A. caudatum and C. argentea, especially in the management of type 2 diabetes.

No MeSH data available.


Related in: MedlinePlus

Scheme for preparation of fractions
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Figure 1: Scheme for preparation of fractions

Mentions: Fractionation of A. caudatum extract was carried out as per Cos et al., with minor modifications [Figure 1].[10] Alcoholic extract was dispersed in 5% w/v citric acid and washed with dichloromethane. Dichloromethane layer was separated and it was concentrated to 1/3rd volume using rotary evaporator at 40°C under reduced pressure. Concentrated dichloromethane layer was partitioned with 90% v/v methanol and petroleum ether (1:1) to get fraction 1, (F1, 9.65 g) and fraction 2, (F2, 7.15 g). Aqueous layer was concentrated to half and pH adjusted to 9.0 with 10% ammonium hydroxide. Aqueous layer was washed with dichloromethane, which gives fraction 3 and fraction 4 (F3, 0.584, and F4, 16.45 g). Same procedure was used for fractionation of C. argentea extract and percentage yield of the fractions were F1 10.15 g, F2 9.76 g, F3 0.593 g, and F4 18.65 g, respectively.


In-vitro α-amylase and α-glucosidase inhibitory activity of Adiantum caudatum Linn. and Celosia argentea Linn. extracts and fractions.

Telagari M, Hullatti K - Indian J Pharmacol (2015 Jul-Aug)

Scheme for preparation of fractions
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4527066&req=5

Figure 1: Scheme for preparation of fractions
Mentions: Fractionation of A. caudatum extract was carried out as per Cos et al., with minor modifications [Figure 1].[10] Alcoholic extract was dispersed in 5% w/v citric acid and washed with dichloromethane. Dichloromethane layer was separated and it was concentrated to 1/3rd volume using rotary evaporator at 40°C under reduced pressure. Concentrated dichloromethane layer was partitioned with 90% v/v methanol and petroleum ether (1:1) to get fraction 1, (F1, 9.65 g) and fraction 2, (F2, 7.15 g). Aqueous layer was concentrated to half and pH adjusted to 9.0 with 10% ammonium hydroxide. Aqueous layer was washed with dichloromethane, which gives fraction 3 and fraction 4 (F3, 0.584, and F4, 16.45 g). Same procedure was used for fractionation of C. argentea extract and percentage yield of the fractions were F1 10.15 g, F2 9.76 g, F3 0.593 g, and F4 18.65 g, respectively.

Bottom Line: Whereas, extracts and remaining fractions of both the plants have shown lesser activity.The results of the present study indicate that, fraction 2 of A. caudatum, rich in triterpenoids and phenolics and fraction 4 of C. argentea, rich in flavonoids, are effective α- amylase and α- glucosidase inhibitors, which may be helpful to reduce the postprandial glucose levels.Hence, further studies may throw light on the antidiabetic potential of A. caudatum and C. argentea, especially in the management of type 2 diabetes.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, KLES College of Pharmacy, Belagavi, Karnataka, India.

ABSTRACT

Objective: The objective of the present study was to provide an in-vitro evidence for the potential inhibitory activity of extracts and fractions of Adiantum caudatum Linn. and Celosia argentea Linn. on α-amylase and α-glucosidase enzymes.

Materials and methods: The plant extracts were prepared, first with cold maceration (70% v/v ethanol) and then by Soxhlation techniques (95% v/v ethanol). Subsequently, the combined extracts were subjected for fractionation. Different concentrations (0.1, 0.2, 0.3, 0.4, and 0.5 mg/ml) of extract and fractions were subjected to α-amylase and α-glucosidase inhibitory assay. The absorbance was measured at 540 and 405 nm using multiplate reader and the percentage of α- amylase and α- glucosidase inhibitory activity and IC₅₀ values of extract and fractions were calculated.

Results: Fraction 2 of A. caudatum and fraction 4 of C. argentea has shown highest α-amylase and α-glucosidase inhibitory potential with IC₅₀ values of 0.241, 0.211 and 0.294, 0.249 mg/ml, respectively, which was comparable with acarbose (0.125 and 0.93 mg/ml). Whereas, extracts and remaining fractions of both the plants have shown lesser activity.

Conclusion: The results of the present study indicate that, fraction 2 of A. caudatum, rich in triterpenoids and phenolics and fraction 4 of C. argentea, rich in flavonoids, are effective α- amylase and α- glucosidase inhibitors, which may be helpful to reduce the postprandial glucose levels. Hence, further studies may throw light on the antidiabetic potential of A. caudatum and C. argentea, especially in the management of type 2 diabetes.

No MeSH data available.


Related in: MedlinePlus