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Calotropis procera: A potential cognition enhancer in scopolamine and electroconvulsive shock-induced amnesia in rats.

Malabade R, Taranalli AD - Indian J Pharmacol (2015 Jul-Aug)

Bottom Line: Dose showing maximum effect in cognitive performance was selected and further evaluated using scopolamine and ECS-induced amnesia model for its effect on neurochemical enzymes and cognitive performance.Further, pretreatment with the selected dose (800 mg/kg) showed significant improvement in transfer latency (P < 0.001, P < 0.01), escape latency (P < 0.05), time spent in target quadrant (P < 0.001) also significant decrease in AChE activity (P < 0.05), β amyloid1-42 level (P < 0.001), and increase in dopamine level (P < 0.01) in rat brain homogenate when compared with scopolamine and ECS disease control groups.Pretreatment with C. procera dry latex (800 mg/kg) produced significant cognition enhancement by improving cognitive performance and decreasing the marker neurochemical enzyme activity in scopolamine and ECS-induced amnesia model.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, KLE University's College of Pharmacy, Belagavi, Karnataka, India.

ABSTRACT

Objectives: Present study evaluates the effect of Calotropis procera (Apocynaceae) dry latex on cognitive function in rats using scopolamine and electroconvulsive shock (ECS) induced amnesia model.

Materials and methods: Male Wistar rats were pretreated with 200, 400 and 800 mg/kg of C. procera dry latex in scopolamine-induced amnesia model. Dose showing maximum effect in cognitive performance was selected and further evaluated using scopolamine and ECS-induced amnesia model for its effect on neurochemical enzymes and cognitive performance. Acetylcholinesterase (AChE) activity, β amyloid1-42, and dopamine level were analyzed, while the cognitive performance was assessed by elevated plus maze, step-through passive avoidance test, and Morris water maze. Simultaneously, C. procera dry latex (25, 50, 100, 250, 500, and 1000 μg/mL) was screened for in vitro AChE inhibition assay.

Results: Pretreatment with (200, 400 and 800 mg/kg) C. procera dry latex shows dose dependent increase in cognitive performance in scopolamine-induced amnesia. Further, pretreatment with the selected dose (800 mg/kg) showed significant improvement in transfer latency (P < 0.001, P < 0.01), escape latency (P < 0.05), time spent in target quadrant (P < 0.001) also significant decrease in AChE activity (P < 0.05), β amyloid1-42 level (P < 0.001), and increase in dopamine level (P < 0.01) in rat brain homogenate when compared with scopolamine and ECS disease control groups. IC50 for C. procera dry latex was found to be <1000 μg/mL.

Conclusions: Pretreatment with C. procera dry latex (800 mg/kg) produced significant cognition enhancement by improving cognitive performance and decreasing the marker neurochemical enzyme activity in scopolamine and ECS-induced amnesia model.

No MeSH data available.


Related in: MedlinePlus

Effect of Calotropis procera (Calotropis procera - 800 mg/kg) on Acetylcholinesterase enzyme activity in scopolamine and electroconvulsive shock-induced amnesia. All values represent mean ± standard error of mean. ***P < 0.001, **P < 0.01, *P < 0.05 versus disease control
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Figure 4: Effect of Calotropis procera (Calotropis procera - 800 mg/kg) on Acetylcholinesterase enzyme activity in scopolamine and electroconvulsive shock-induced amnesia. All values represent mean ± standard error of mean. ***P < 0.001, **P < 0.01, *P < 0.05 versus disease control

Mentions: Pretreatment with C. procera dry latex (800 mg/kg) significantly reduced (P < 0.05) the AChE activity as compared to disease control groups (P < 0.01). Mentat also significantly decreased AChE activity in Sco + Mentat (P < 0.01) group and ECS + Mentat (P < 0.05) as indicated in Figure 4.


Calotropis procera: A potential cognition enhancer in scopolamine and electroconvulsive shock-induced amnesia in rats.

Malabade R, Taranalli AD - Indian J Pharmacol (2015 Jul-Aug)

Effect of Calotropis procera (Calotropis procera - 800 mg/kg) on Acetylcholinesterase enzyme activity in scopolamine and electroconvulsive shock-induced amnesia. All values represent mean ± standard error of mean. ***P < 0.001, **P < 0.01, *P < 0.05 versus disease control
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4527065&req=5

Figure 4: Effect of Calotropis procera (Calotropis procera - 800 mg/kg) on Acetylcholinesterase enzyme activity in scopolamine and electroconvulsive shock-induced amnesia. All values represent mean ± standard error of mean. ***P < 0.001, **P < 0.01, *P < 0.05 versus disease control
Mentions: Pretreatment with C. procera dry latex (800 mg/kg) significantly reduced (P < 0.05) the AChE activity as compared to disease control groups (P < 0.01). Mentat also significantly decreased AChE activity in Sco + Mentat (P < 0.01) group and ECS + Mentat (P < 0.05) as indicated in Figure 4.

Bottom Line: Dose showing maximum effect in cognitive performance was selected and further evaluated using scopolamine and ECS-induced amnesia model for its effect on neurochemical enzymes and cognitive performance.Further, pretreatment with the selected dose (800 mg/kg) showed significant improvement in transfer latency (P < 0.001, P < 0.01), escape latency (P < 0.05), time spent in target quadrant (P < 0.001) also significant decrease in AChE activity (P < 0.05), β amyloid1-42 level (P < 0.001), and increase in dopamine level (P < 0.01) in rat brain homogenate when compared with scopolamine and ECS disease control groups.Pretreatment with C. procera dry latex (800 mg/kg) produced significant cognition enhancement by improving cognitive performance and decreasing the marker neurochemical enzyme activity in scopolamine and ECS-induced amnesia model.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, KLE University's College of Pharmacy, Belagavi, Karnataka, India.

ABSTRACT

Objectives: Present study evaluates the effect of Calotropis procera (Apocynaceae) dry latex on cognitive function in rats using scopolamine and electroconvulsive shock (ECS) induced amnesia model.

Materials and methods: Male Wistar rats were pretreated with 200, 400 and 800 mg/kg of C. procera dry latex in scopolamine-induced amnesia model. Dose showing maximum effect in cognitive performance was selected and further evaluated using scopolamine and ECS-induced amnesia model for its effect on neurochemical enzymes and cognitive performance. Acetylcholinesterase (AChE) activity, β amyloid1-42, and dopamine level were analyzed, while the cognitive performance was assessed by elevated plus maze, step-through passive avoidance test, and Morris water maze. Simultaneously, C. procera dry latex (25, 50, 100, 250, 500, and 1000 μg/mL) was screened for in vitro AChE inhibition assay.

Results: Pretreatment with (200, 400 and 800 mg/kg) C. procera dry latex shows dose dependent increase in cognitive performance in scopolamine-induced amnesia. Further, pretreatment with the selected dose (800 mg/kg) showed significant improvement in transfer latency (P < 0.001, P < 0.01), escape latency (P < 0.05), time spent in target quadrant (P < 0.001) also significant decrease in AChE activity (P < 0.05), β amyloid1-42 level (P < 0.001), and increase in dopamine level (P < 0.01) in rat brain homogenate when compared with scopolamine and ECS disease control groups. IC50 for C. procera dry latex was found to be <1000 μg/mL.

Conclusions: Pretreatment with C. procera dry latex (800 mg/kg) produced significant cognition enhancement by improving cognitive performance and decreasing the marker neurochemical enzyme activity in scopolamine and ECS-induced amnesia model.

No MeSH data available.


Related in: MedlinePlus