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Immunoprophylactic potential of wheat grass extract on benzene-induced leukemia: An in vivo study on murine model.

Khan N, Ganeshpurkar A, Dubey N, Bansal D - Indian J Pharmacol (2015 Jul-Aug)

Bottom Line: Leukemia was successfully induced in Wister rats by intravenous injection of benzene.Outcomes of hematological parameters in various experimental groups of murine model demonstrated antileukemic effect of extract.Further isolation, structural characterization of active constituents is necessary to extrapolate the mechanism of action.

View Article: PubMed Central - PubMed

Affiliation: Drug Discovery Laboratory, Shri Ram Institute of Technology-Pharmacy, Jabalpur, Madhya Pradesh, India.

ABSTRACT

Objectives: Wheat grass (Triticum aestivum) is a gift of nature given to mankind. A number of scientific research on wheatgrass establishes its anticancer and antioxidant potential. Current work was focused to determine antileukemic effect of wheat grass.

Materials and methods: The commercial wheatgrass powder was extracted with 95% of methanol. Methanol extract of wheat grass was studied for acute oral toxicity as per revised Organization for Economic Cooperation and Development Guidelines number 423. Leukemia was successfully induced in Wister rats by intravenous injection of benzene. The blood was collected and analyzed for hematological parameters. Phagocytotic activity of the extract was determined.

Results: Phytochemical screening revealed the presence of flavonoids, phenolics, carbohydrates, and amino acids. From acute toxicity studies, it was found that the methanol extract of wheatgrass was safe up to a dose level of 2000 mg/kg of body weight. Outcomes of hematological parameters in various experimental groups of murine model demonstrated antileukemic effect of extract. Methanol extract of wheatgrass aroused the process of phagocytosis of killed Candida albicans and also demonstrated a significant chemotactic activity at all tested concentrations.

Conclusion: In the current work, methanol extract of wheat grass demonstrated antileukemic potential that might be due to the presence of flavonoids and polyphenolics in it. Further isolation, structural characterization of active constituents is necessary to extrapolate the mechanism of action.

No MeSH data available.


Related in: MedlinePlus

Number of neutrophils per field after treatment with methanol extract of wheat grass determined by neutrophil locomotion and chemotactic activity. The results are expressed as mean ± standard error of mean. Statistical comparison was performed using analysis of variance followed by Bonferroni's test (*P < 0.05)
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Figure 1: Number of neutrophils per field after treatment with methanol extract of wheat grass determined by neutrophil locomotion and chemotactic activity. The results are expressed as mean ± standard error of mean. Statistical comparison was performed using analysis of variance followed by Bonferroni's test (*P < 0.05)

Mentions: Methanol extract of wheat grass demonstrated a significant (P < 0.05) chemotactic activity at all tested concentrations. Mean number of neutrophils per field for extract was found to be 138.02, 129.9, 122.3 at concentrations of 1000 μg/ml, 100 μg/ml, and 40 μg/ml, respectively, when compared to the standard-casein [Figure 1].


Immunoprophylactic potential of wheat grass extract on benzene-induced leukemia: An in vivo study on murine model.

Khan N, Ganeshpurkar A, Dubey N, Bansal D - Indian J Pharmacol (2015 Jul-Aug)

Number of neutrophils per field after treatment with methanol extract of wheat grass determined by neutrophil locomotion and chemotactic activity. The results are expressed as mean ± standard error of mean. Statistical comparison was performed using analysis of variance followed by Bonferroni's test (*P < 0.05)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4527060&req=5

Figure 1: Number of neutrophils per field after treatment with methanol extract of wheat grass determined by neutrophil locomotion and chemotactic activity. The results are expressed as mean ± standard error of mean. Statistical comparison was performed using analysis of variance followed by Bonferroni's test (*P < 0.05)
Mentions: Methanol extract of wheat grass demonstrated a significant (P < 0.05) chemotactic activity at all tested concentrations. Mean number of neutrophils per field for extract was found to be 138.02, 129.9, 122.3 at concentrations of 1000 μg/ml, 100 μg/ml, and 40 μg/ml, respectively, when compared to the standard-casein [Figure 1].

Bottom Line: Leukemia was successfully induced in Wister rats by intravenous injection of benzene.Outcomes of hematological parameters in various experimental groups of murine model demonstrated antileukemic effect of extract.Further isolation, structural characterization of active constituents is necessary to extrapolate the mechanism of action.

View Article: PubMed Central - PubMed

Affiliation: Drug Discovery Laboratory, Shri Ram Institute of Technology-Pharmacy, Jabalpur, Madhya Pradesh, India.

ABSTRACT

Objectives: Wheat grass (Triticum aestivum) is a gift of nature given to mankind. A number of scientific research on wheatgrass establishes its anticancer and antioxidant potential. Current work was focused to determine antileukemic effect of wheat grass.

Materials and methods: The commercial wheatgrass powder was extracted with 95% of methanol. Methanol extract of wheat grass was studied for acute oral toxicity as per revised Organization for Economic Cooperation and Development Guidelines number 423. Leukemia was successfully induced in Wister rats by intravenous injection of benzene. The blood was collected and analyzed for hematological parameters. Phagocytotic activity of the extract was determined.

Results: Phytochemical screening revealed the presence of flavonoids, phenolics, carbohydrates, and amino acids. From acute toxicity studies, it was found that the methanol extract of wheatgrass was safe up to a dose level of 2000 mg/kg of body weight. Outcomes of hematological parameters in various experimental groups of murine model demonstrated antileukemic effect of extract. Methanol extract of wheatgrass aroused the process of phagocytosis of killed Candida albicans and also demonstrated a significant chemotactic activity at all tested concentrations.

Conclusion: In the current work, methanol extract of wheat grass demonstrated antileukemic potential that might be due to the presence of flavonoids and polyphenolics in it. Further isolation, structural characterization of active constituents is necessary to extrapolate the mechanism of action.

No MeSH data available.


Related in: MedlinePlus