Limits...
Household Transmission of Zoonotic Influenza Viruses in a Cohort of Egyptian Poultry Growers.

El Rifay AS, Elabd MA, Abu Zeid D, Gomaa MR, Tang L, McKenzie PP, Webby RJ, Ali MA, Kayali G - JMIR Res Protoc (2015)

Bottom Line: We aim to measure the incidence of avian influenza infection in Egyptians exposed to poultry, study risk factors of infection, study the resulting immune response, study household transmission rates, and characterize the viruses causing infections.The nurse will also obtain 2x 3-ml blood samples, one for serology, and another for isolating peripheral blood mononuclear cells.This may enhance public health efforts aimed at reducing this burden.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center of Excellence for Influenza Viruses, National Research Centre, Giza, Egypt.

ABSTRACT

Background: The highly pathogenic avian influenza H5N1 viruses and the low pathogenic H9N2 viruses are enzootic in Egyptian poultry. Several cases of human infection with H5N1 were reported in Egypt. We previously determined that the seroprevalence of H5N1 antibodies in Egyptians exposed to poultry is 2.1% (15/708), suggesting that mild or subclinical infections with this virus occur. We aim to measure the incidence of avian influenza infection in Egyptians exposed to poultry, study risk factors of infection, study the resulting immune response, study household transmission rates, and characterize the viruses causing infections.

Objective: The objective of the study is to design a 7-year, prospective, household-based cohort investigation to determine incidence and household transmission of avian influenza viruses in humans exposed to poultry.

Methods: At baseline, we will collect sera to measure antibodies against influenza A. Field nurses will visit enrolled subjects at least weekly to check for influenza-like illness symptoms and verify influenza infection by a point of care rapid test. From subjects with influenza infection and their household contacts, we will collect nasal swabs, throat swabs, and nasal washes to characterize the antigenic and genetic makeup of influenza viruses infecting humans. The nurse will also obtain 2x 3-ml blood samples, one for serology, and another for isolating peripheral blood mononuclear cells.

Results: Results from this cohort will enhance our understanding of the transmission of avian influenza viruses to humans in a country where such viruses are enzootic.

Conclusions: This may enhance public health efforts aimed at reducing this burden.

No MeSH data available.


Related in: MedlinePlus

Schematic of the study design.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4526956&req=5

figure2: Schematic of the study design.

Mentions: Figure 2 shows a schematic of the study design. We designed a 7-year prospective, household-based, cohort study to determine incidence and household transmission of AIV in humans exposed to poultry. At the start of the study, study staff will obtain informed consent, determine eligibility, and collect a serum sample to establish baseline levels of antibodies against AIV. Questionnaires specifically tailored for this study will be used (see Multimedia Appendix 1). Data on the demographics, health status, use of seasonal influenza vaccines, poultry exposure, and use of poultry influenza vaccines will be collected.


Household Transmission of Zoonotic Influenza Viruses in a Cohort of Egyptian Poultry Growers.

El Rifay AS, Elabd MA, Abu Zeid D, Gomaa MR, Tang L, McKenzie PP, Webby RJ, Ali MA, Kayali G - JMIR Res Protoc (2015)

Schematic of the study design.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4526956&req=5

figure2: Schematic of the study design.
Mentions: Figure 2 shows a schematic of the study design. We designed a 7-year prospective, household-based, cohort study to determine incidence and household transmission of AIV in humans exposed to poultry. At the start of the study, study staff will obtain informed consent, determine eligibility, and collect a serum sample to establish baseline levels of antibodies against AIV. Questionnaires specifically tailored for this study will be used (see Multimedia Appendix 1). Data on the demographics, health status, use of seasonal influenza vaccines, poultry exposure, and use of poultry influenza vaccines will be collected.

Bottom Line: We aim to measure the incidence of avian influenza infection in Egyptians exposed to poultry, study risk factors of infection, study the resulting immune response, study household transmission rates, and characterize the viruses causing infections.The nurse will also obtain 2x 3-ml blood samples, one for serology, and another for isolating peripheral blood mononuclear cells.This may enhance public health efforts aimed at reducing this burden.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center of Excellence for Influenza Viruses, National Research Centre, Giza, Egypt.

ABSTRACT

Background: The highly pathogenic avian influenza H5N1 viruses and the low pathogenic H9N2 viruses are enzootic in Egyptian poultry. Several cases of human infection with H5N1 were reported in Egypt. We previously determined that the seroprevalence of H5N1 antibodies in Egyptians exposed to poultry is 2.1% (15/708), suggesting that mild or subclinical infections with this virus occur. We aim to measure the incidence of avian influenza infection in Egyptians exposed to poultry, study risk factors of infection, study the resulting immune response, study household transmission rates, and characterize the viruses causing infections.

Objective: The objective of the study is to design a 7-year, prospective, household-based cohort investigation to determine incidence and household transmission of avian influenza viruses in humans exposed to poultry.

Methods: At baseline, we will collect sera to measure antibodies against influenza A. Field nurses will visit enrolled subjects at least weekly to check for influenza-like illness symptoms and verify influenza infection by a point of care rapid test. From subjects with influenza infection and their household contacts, we will collect nasal swabs, throat swabs, and nasal washes to characterize the antigenic and genetic makeup of influenza viruses infecting humans. The nurse will also obtain 2x 3-ml blood samples, one for serology, and another for isolating peripheral blood mononuclear cells.

Results: Results from this cohort will enhance our understanding of the transmission of avian influenza viruses to humans in a country where such viruses are enzootic.

Conclusions: This may enhance public health efforts aimed at reducing this burden.

No MeSH data available.


Related in: MedlinePlus