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Modulation of Arabidopsis and monocot root architecture by CLAVATA3/EMBRYO SURROUNDING REGION 26 peptide.

Czyzewicz N, Shi CL, Vu LD, Van De Cotte B, Hodgman C, Butenko MA, Smet ID - J. Exp. Bot. (2015)

Bottom Line: Using chemically synthesized peptide variants, it was found that CLE26 plays an important role in regulating A. thaliana root architecture and interacts with auxin signalling.In addition, through alanine scanning and in silico structural modelling, key residues in the CLE26 peptide sequence that affect its activity are pinpointed.Finally, some interesting similarities and differences regarding the role of CLE26 in regulating monocot root architecture are presented.

View Article: PubMed Central - PubMed

Affiliation: Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Leicestershire LE12 5RD, UK.

No MeSH data available.


Characterization of the cle26-1 mutant. (A) Position of the T-DNA insertion in two splicing variants. Primers used in qPCR are indicated. (B) CLE26 expression level as determined by qPCR in cle26-1 roots. (C–E) Root phenotype of cle26-1 at 9 d after germination. Representative pictures of wild-type and cle26-1 seedlings (C). (D, E) Quantification of primary root length (D) and emerged lateral root density (E) for cle26-1 seedlings. The graphs indicate the mean ±SE. Statistical significance (Student’s t-test) compared with Col-0 is indicated: *P<0.05. Scale bar=1cm. (This figure is available in colour at JXB online.)
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Figure 10: Characterization of the cle26-1 mutant. (A) Position of the T-DNA insertion in two splicing variants. Primers used in qPCR are indicated. (B) CLE26 expression level as determined by qPCR in cle26-1 roots. (C–E) Root phenotype of cle26-1 at 9 d after germination. Representative pictures of wild-type and cle26-1 seedlings (C). (D, E) Quantification of primary root length (D) and emerged lateral root density (E) for cle26-1 seedlings. The graphs indicate the mean ±SE. Statistical significance (Student’s t-test) compared with Col-0 is indicated: *P<0.05. Scale bar=1cm. (This figure is available in colour at JXB online.)

Mentions: To explore further the role of CLE26 in mediating root architecture, a T-DNA insertion line, which was named cle26-1, was analysed (Fig. 10A). Interestingly, CLE26 has two splicing variants that are both expressed in the root (Fig. 10A; Supplementary Fig. S9 at JXB online). qPCR was performed to examine CLE26 mRNA transcript levels (using a primer pair that captures both CLE26.1 and CLE26.2) in cle26-1, and an ~80% decrease in CLE26 expression was observed (Fig. 10B). Then the primary root length and lateral root density of cle26-1 were analysed. This revealed a mild, significant increase with respect to primary root length, and no difference in the level of emerged lateral root density (Fig. 10C–E). Overall, it appears that CLE26 impacts on primary root length and that its effect on lateral root density may be compensated for by other closely related signalling pathways or by genetic redundancy with other CLE peptides.


Modulation of Arabidopsis and monocot root architecture by CLAVATA3/EMBRYO SURROUNDING REGION 26 peptide.

Czyzewicz N, Shi CL, Vu LD, Van De Cotte B, Hodgman C, Butenko MA, Smet ID - J. Exp. Bot. (2015)

Characterization of the cle26-1 mutant. (A) Position of the T-DNA insertion in two splicing variants. Primers used in qPCR are indicated. (B) CLE26 expression level as determined by qPCR in cle26-1 roots. (C–E) Root phenotype of cle26-1 at 9 d after germination. Representative pictures of wild-type and cle26-1 seedlings (C). (D, E) Quantification of primary root length (D) and emerged lateral root density (E) for cle26-1 seedlings. The graphs indicate the mean ±SE. Statistical significance (Student’s t-test) compared with Col-0 is indicated: *P<0.05. Scale bar=1cm. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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getmorefigures.php?uid=PMC4526925&req=5

Figure 10: Characterization of the cle26-1 mutant. (A) Position of the T-DNA insertion in two splicing variants. Primers used in qPCR are indicated. (B) CLE26 expression level as determined by qPCR in cle26-1 roots. (C–E) Root phenotype of cle26-1 at 9 d after germination. Representative pictures of wild-type and cle26-1 seedlings (C). (D, E) Quantification of primary root length (D) and emerged lateral root density (E) for cle26-1 seedlings. The graphs indicate the mean ±SE. Statistical significance (Student’s t-test) compared with Col-0 is indicated: *P<0.05. Scale bar=1cm. (This figure is available in colour at JXB online.)
Mentions: To explore further the role of CLE26 in mediating root architecture, a T-DNA insertion line, which was named cle26-1, was analysed (Fig. 10A). Interestingly, CLE26 has two splicing variants that are both expressed in the root (Fig. 10A; Supplementary Fig. S9 at JXB online). qPCR was performed to examine CLE26 mRNA transcript levels (using a primer pair that captures both CLE26.1 and CLE26.2) in cle26-1, and an ~80% decrease in CLE26 expression was observed (Fig. 10B). Then the primary root length and lateral root density of cle26-1 were analysed. This revealed a mild, significant increase with respect to primary root length, and no difference in the level of emerged lateral root density (Fig. 10C–E). Overall, it appears that CLE26 impacts on primary root length and that its effect on lateral root density may be compensated for by other closely related signalling pathways or by genetic redundancy with other CLE peptides.

Bottom Line: Using chemically synthesized peptide variants, it was found that CLE26 plays an important role in regulating A. thaliana root architecture and interacts with auxin signalling.In addition, through alanine scanning and in silico structural modelling, key residues in the CLE26 peptide sequence that affect its activity are pinpointed.Finally, some interesting similarities and differences regarding the role of CLE26 in regulating monocot root architecture are presented.

View Article: PubMed Central - PubMed

Affiliation: Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Leicestershire LE12 5RD, UK.

No MeSH data available.