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Modulation of Arabidopsis and monocot root architecture by CLAVATA3/EMBRYO SURROUNDING REGION 26 peptide.

Czyzewicz N, Shi CL, Vu LD, Van De Cotte B, Hodgman C, Butenko MA, Smet ID - J. Exp. Bot. (2015)

Bottom Line: Using chemically synthesized peptide variants, it was found that CLE26 plays an important role in regulating A. thaliana root architecture and interacts with auxin signalling.In addition, through alanine scanning and in silico structural modelling, key residues in the CLE26 peptide sequence that affect its activity are pinpointed.Finally, some interesting similarities and differences regarding the role of CLE26 in regulating monocot root architecture are presented.

View Article: PubMed Central - PubMed

Affiliation: Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Leicestershire LE12 5RD, UK.

No MeSH data available.


Effect of AtCLE26p and BdCLE26p on wheat, B. distachyon, and A. thaliana. (A, B) Representative pictures are shown for wheat (A) and B. distachyon (B) at 12 d after germination. (C, D) Quantification of B. distachyon seedling primary root length (C) and emerged lateral root density (D). (E, F) Quantification of A. thaliana seedling primary root length (C) and emerged lateral root density (D). The bar graphs indicate the mean ±SE. Statistical significance (Student’s t-test) compared with no peptide treatment is indicated: ***P<0.01. Scale bar=1cm.
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Figure 6: Effect of AtCLE26p and BdCLE26p on wheat, B. distachyon, and A. thaliana. (A, B) Representative pictures are shown for wheat (A) and B. distachyon (B) at 12 d after germination. (C, D) Quantification of B. distachyon seedling primary root length (C) and emerged lateral root density (D). (E, F) Quantification of A. thaliana seedling primary root length (C) and emerged lateral root density (D). The bar graphs indicate the mean ±SE. Statistical significance (Student’s t-test) compared with no peptide treatment is indicated: ***P<0.01. Scale bar=1cm.

Mentions: To investigate whether the effect of CLE26p observed in A. thaliana also extends to monocots, the primary root length of CLE26p-treated B. distachyon (Bd21) and wheat was analysed. CLE26p treatment of B. distachyon and wheat resulted in a short primary root compared with the untreated control (Fig. 6A–C). These results were similar to those in A. thaliana and suggested that an orthologue of AtCLE26 may also be involved in regulating primary root growth in monocots. However, in contrast to A. thaliana, CLE26p application failed to induce any obvious change to lateral root density in B. distachyon (Fig. 6D).


Modulation of Arabidopsis and monocot root architecture by CLAVATA3/EMBRYO SURROUNDING REGION 26 peptide.

Czyzewicz N, Shi CL, Vu LD, Van De Cotte B, Hodgman C, Butenko MA, Smet ID - J. Exp. Bot. (2015)

Effect of AtCLE26p and BdCLE26p on wheat, B. distachyon, and A. thaliana. (A, B) Representative pictures are shown for wheat (A) and B. distachyon (B) at 12 d after germination. (C, D) Quantification of B. distachyon seedling primary root length (C) and emerged lateral root density (D). (E, F) Quantification of A. thaliana seedling primary root length (C) and emerged lateral root density (D). The bar graphs indicate the mean ±SE. Statistical significance (Student’s t-test) compared with no peptide treatment is indicated: ***P<0.01. Scale bar=1cm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526925&req=5

Figure 6: Effect of AtCLE26p and BdCLE26p on wheat, B. distachyon, and A. thaliana. (A, B) Representative pictures are shown for wheat (A) and B. distachyon (B) at 12 d after germination. (C, D) Quantification of B. distachyon seedling primary root length (C) and emerged lateral root density (D). (E, F) Quantification of A. thaliana seedling primary root length (C) and emerged lateral root density (D). The bar graphs indicate the mean ±SE. Statistical significance (Student’s t-test) compared with no peptide treatment is indicated: ***P<0.01. Scale bar=1cm.
Mentions: To investigate whether the effect of CLE26p observed in A. thaliana also extends to monocots, the primary root length of CLE26p-treated B. distachyon (Bd21) and wheat was analysed. CLE26p treatment of B. distachyon and wheat resulted in a short primary root compared with the untreated control (Fig. 6A–C). These results were similar to those in A. thaliana and suggested that an orthologue of AtCLE26 may also be involved in regulating primary root growth in monocots. However, in contrast to A. thaliana, CLE26p application failed to induce any obvious change to lateral root density in B. distachyon (Fig. 6D).

Bottom Line: Using chemically synthesized peptide variants, it was found that CLE26 plays an important role in regulating A. thaliana root architecture and interacts with auxin signalling.In addition, through alanine scanning and in silico structural modelling, key residues in the CLE26 peptide sequence that affect its activity are pinpointed.Finally, some interesting similarities and differences regarding the role of CLE26 in regulating monocot root architecture are presented.

View Article: PubMed Central - PubMed

Affiliation: Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Leicestershire LE12 5RD, UK.

No MeSH data available.