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Modulation of Arabidopsis and monocot root architecture by CLAVATA3/EMBRYO SURROUNDING REGION 26 peptide.

Czyzewicz N, Shi CL, Vu LD, Van De Cotte B, Hodgman C, Butenko MA, Smet ID - J. Exp. Bot. (2015)

Bottom Line: Using chemically synthesized peptide variants, it was found that CLE26 plays an important role in regulating A. thaliana root architecture and interacts with auxin signalling.In addition, through alanine scanning and in silico structural modelling, key residues in the CLE26 peptide sequence that affect its activity are pinpointed.Finally, some interesting similarities and differences regarding the role of CLE26 in regulating monocot root architecture are presented.

View Article: PubMed Central - PubMed

Affiliation: Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Leicestershire LE12 5RD, UK.

No MeSH data available.


CLE expression visualized through pCLE::GUS lines. (A, B) pCLE26::GUS in the primary root tip: (A) whole mount; (B) transverse section in the basal meristem. (C–F) pCLE::GUS during early stages of A. thaliana lateral root development: (C) pCLE27::GUS; (D) pCLE1::GUS; (E) pCLE4::GUS; (F) pCLE7::GUS. A red asterisk indicates the position of the lateral root primordium. Seedling age, 5–7 d after germination.
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Figure 1: CLE expression visualized through pCLE::GUS lines. (A, B) pCLE26::GUS in the primary root tip: (A) whole mount; (B) transverse section in the basal meristem. (C–F) pCLE::GUS during early stages of A. thaliana lateral root development: (C) pCLE27::GUS; (D) pCLE1::GUS; (E) pCLE4::GUS; (F) pCLE7::GUS. A red asterisk indicates the position of the lateral root primordium. Seedling age, 5–7 d after germination.

Mentions: Based on the observed CLE expression patterns, a subset of CLEs was used for further analyses. Expression of pCLE6::GUS, pCLE22::GUS, pCLE25::GUS, pCLE26:: GUS, and pCLE27::GUS was observed in the basal meristem, with CLE6, CLE22, CLE25, and CLE26 expression seemingly restricted to the stele and with CLE26 being the most strongly expressed (Fig. 1A; Supplementary Fig. S1 at JXB online). The latter expression patterns may indicate a role in lateral root patterning via establishment of the vascular pattern and/or priming of pericycle cells (De Smet et al., 2007; Parizot et al., 2008, 2012). During the early stages of lateral root development, pCLE27::GUS was specifically expressed in the asymmetrically dividing pericycle cells (Fig. 1C). Based on this expression pattern (in the core of the lateral root initiation site), it was hypothesized that CLE27 could be a positive regulator of early stages of lateral root development. In contrast, during lateral root initiation and development, pCLE1::GUS, pCLE4::GUS, and pCLE7::GUS were expressed in the vasculature and pericycle, but excluded from the developing primordium (Fig. 1D–F). Based on these expression patterns (excluded from the lateral root initiation site), it is hypothesized that these CLEs may be negative regulators of early stages of lateral root development.


Modulation of Arabidopsis and monocot root architecture by CLAVATA3/EMBRYO SURROUNDING REGION 26 peptide.

Czyzewicz N, Shi CL, Vu LD, Van De Cotte B, Hodgman C, Butenko MA, Smet ID - J. Exp. Bot. (2015)

CLE expression visualized through pCLE::GUS lines. (A, B) pCLE26::GUS in the primary root tip: (A) whole mount; (B) transverse section in the basal meristem. (C–F) pCLE::GUS during early stages of A. thaliana lateral root development: (C) pCLE27::GUS; (D) pCLE1::GUS; (E) pCLE4::GUS; (F) pCLE7::GUS. A red asterisk indicates the position of the lateral root primordium. Seedling age, 5–7 d after germination.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526925&req=5

Figure 1: CLE expression visualized through pCLE::GUS lines. (A, B) pCLE26::GUS in the primary root tip: (A) whole mount; (B) transverse section in the basal meristem. (C–F) pCLE::GUS during early stages of A. thaliana lateral root development: (C) pCLE27::GUS; (D) pCLE1::GUS; (E) pCLE4::GUS; (F) pCLE7::GUS. A red asterisk indicates the position of the lateral root primordium. Seedling age, 5–7 d after germination.
Mentions: Based on the observed CLE expression patterns, a subset of CLEs was used for further analyses. Expression of pCLE6::GUS, pCLE22::GUS, pCLE25::GUS, pCLE26:: GUS, and pCLE27::GUS was observed in the basal meristem, with CLE6, CLE22, CLE25, and CLE26 expression seemingly restricted to the stele and with CLE26 being the most strongly expressed (Fig. 1A; Supplementary Fig. S1 at JXB online). The latter expression patterns may indicate a role in lateral root patterning via establishment of the vascular pattern and/or priming of pericycle cells (De Smet et al., 2007; Parizot et al., 2008, 2012). During the early stages of lateral root development, pCLE27::GUS was specifically expressed in the asymmetrically dividing pericycle cells (Fig. 1C). Based on this expression pattern (in the core of the lateral root initiation site), it was hypothesized that CLE27 could be a positive regulator of early stages of lateral root development. In contrast, during lateral root initiation and development, pCLE1::GUS, pCLE4::GUS, and pCLE7::GUS were expressed in the vasculature and pericycle, but excluded from the developing primordium (Fig. 1D–F). Based on these expression patterns (excluded from the lateral root initiation site), it is hypothesized that these CLEs may be negative regulators of early stages of lateral root development.

Bottom Line: Using chemically synthesized peptide variants, it was found that CLE26 plays an important role in regulating A. thaliana root architecture and interacts with auxin signalling.In addition, through alanine scanning and in silico structural modelling, key residues in the CLE26 peptide sequence that affect its activity are pinpointed.Finally, some interesting similarities and differences regarding the role of CLE26 in regulating monocot root architecture are presented.

View Article: PubMed Central - PubMed

Affiliation: Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Leicestershire LE12 5RD, UK.

No MeSH data available.