Limits...
Genome-wide annotation and characterization of CLAVATA/ESR (CLE) peptide hormones of soybean (Glycine max) and common bean (Phaseolus vulgaris), and their orthologues of Arabidopsis thaliana.

Hastwell AH, Gresshoff PM, Ferguson BJ - J. Exp. Bot. (2015)

Bottom Line: The soybean CLE pre-propeptide family was further analysed and separated into seven distinct groups based on structure, with groupings strongly associated with the CLE domain sequence and function.Transcriptional evidence was also used to provide further insight into the location and function of all CLE peptide-encoding members currently available in gene atlases for the three species.Taken together, this in-depth analysis helped to identify and categorize the complete CLE peptide families of soybean and common bean, established gene orthologues within the two legume species, and Arabidopsis, and provided a platform to help compare, contrast, and identify the function of critical CLE peptide hormones in plant development.

View Article: PubMed Central - PubMed

Affiliation: Centre for Integrative Legume Research, School of Agricultural and Food Sciences, The University of Queensland, St Lucia, Brisbane, Queensland, 4072, Australia.

No MeSH data available.


Multiple sequence alignment of soybean (Glycine max) CLE pre-propeptides. Homeologous copies consistently align together, as do other closely related sequences. Shading of amino acid residues represents conservation, with the darker the shading the more highly conserved the residues. The CLE domain and the leucine-rich region of the signal peptide domain exhibit the greatest degree of conservation across the entire pre-propeptide family. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4526924&req=5

Figure 1: Multiple sequence alignment of soybean (Glycine max) CLE pre-propeptides. Homeologous copies consistently align together, as do other closely related sequences. Shading of amino acid residues represents conservation, with the darker the shading the more highly conserved the residues. The CLE domain and the leucine-rich region of the signal peptide domain exhibit the greatest degree of conservation across the entire pre-propeptide family. (This figure is available in colour at JXB online.)

Mentions: To identify CLE peptide-encoding genes in soybean and common bean, a genome-wide analysis was performed involving multiple BLAST queries, followed by manual validation and the removal of false positives (i.e. no CLE domain). This resulted in the identification of 84 distinct soybean genes and 44 distinct common bean genes (Figs 1, 2; Tables 1, 2). BLAST queries were based on all known soybean CLE genes, and some Arabidopsis genes, and involved searching with both pre-propeptide and CLE domain sequences to enhance the likelihood of detecting all CLE peptide-encoding genes in the two genomes.


Genome-wide annotation and characterization of CLAVATA/ESR (CLE) peptide hormones of soybean (Glycine max) and common bean (Phaseolus vulgaris), and their orthologues of Arabidopsis thaliana.

Hastwell AH, Gresshoff PM, Ferguson BJ - J. Exp. Bot. (2015)

Multiple sequence alignment of soybean (Glycine max) CLE pre-propeptides. Homeologous copies consistently align together, as do other closely related sequences. Shading of amino acid residues represents conservation, with the darker the shading the more highly conserved the residues. The CLE domain and the leucine-rich region of the signal peptide domain exhibit the greatest degree of conservation across the entire pre-propeptide family. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526924&req=5

Figure 1: Multiple sequence alignment of soybean (Glycine max) CLE pre-propeptides. Homeologous copies consistently align together, as do other closely related sequences. Shading of amino acid residues represents conservation, with the darker the shading the more highly conserved the residues. The CLE domain and the leucine-rich region of the signal peptide domain exhibit the greatest degree of conservation across the entire pre-propeptide family. (This figure is available in colour at JXB online.)
Mentions: To identify CLE peptide-encoding genes in soybean and common bean, a genome-wide analysis was performed involving multiple BLAST queries, followed by manual validation and the removal of false positives (i.e. no CLE domain). This resulted in the identification of 84 distinct soybean genes and 44 distinct common bean genes (Figs 1, 2; Tables 1, 2). BLAST queries were based on all known soybean CLE genes, and some Arabidopsis genes, and involved searching with both pre-propeptide and CLE domain sequences to enhance the likelihood of detecting all CLE peptide-encoding genes in the two genomes.

Bottom Line: The soybean CLE pre-propeptide family was further analysed and separated into seven distinct groups based on structure, with groupings strongly associated with the CLE domain sequence and function.Transcriptional evidence was also used to provide further insight into the location and function of all CLE peptide-encoding members currently available in gene atlases for the three species.Taken together, this in-depth analysis helped to identify and categorize the complete CLE peptide families of soybean and common bean, established gene orthologues within the two legume species, and Arabidopsis, and provided a platform to help compare, contrast, and identify the function of critical CLE peptide hormones in plant development.

View Article: PubMed Central - PubMed

Affiliation: Centre for Integrative Legume Research, School of Agricultural and Food Sciences, The University of Queensland, St Lucia, Brisbane, Queensland, 4072, Australia.

No MeSH data available.