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Expanding the repertoire of secretory peptides controlling root development with comparative genome analysis and functional assays.

Ghorbani S, Lin YC, Parizot B, Fernandez A, Njo MF, Van de Peer Y, Beeckman T, Hilson P - J. Exp. Bot. (2015)

Bottom Line: Based on structural features that characterize SSP families known to take part in postembryonic development, this comparative genome analysis resulted in the identification of genes coding for oligopeptides potentially involved in cell-to-cell communication.The strategy used in the study, combining comparative genomics, transcriptome meta-analysis and peptide functional assays in planta, pinpoints factors potentially involved in non-cell-autonomous regulatory mechanisms.A similar approach can be implemented in different species for the study of a wide range of developmental programmes.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.

No MeSH data available.


LR-related phenotypes induced by the f919-2 peptide. (A) Distribution of LR developmental stages in roots 12 days after germination. I–VII, primordium stages; NE, non-emerged primordia; E, emerged LRs; total, total number of LRs; np, no peptide; r, randomized peptide. Results for one of two independent experiments are shown (see Materials and Methods). Error bars represent the 95% confidence interval. Asterisks indicate significant differences compared with the no-peptide control (*** P < 0.001). (B, C) Differential interference contrast images of representative stage V LRP-treated (C) or not treated (B) with the f919-2 peptide (10 μM). (D) Relative distribution of the normal and flattened stage V LRP. D, dome-shaped primordia (black); F, flattened primordia (grey) (n = 15–48).
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Figure 7: LR-related phenotypes induced by the f919-2 peptide. (A) Distribution of LR developmental stages in roots 12 days after germination. I–VII, primordium stages; NE, non-emerged primordia; E, emerged LRs; total, total number of LRs; np, no peptide; r, randomized peptide. Results for one of two independent experiments are shown (see Materials and Methods). Error bars represent the 95% confidence interval. Asterisks indicate significant differences compared with the no-peptide control (*** P < 0.001). (B, C) Differential interference contrast images of representative stage V LRP-treated (C) or not treated (B) with the f919-2 peptide (10 μM). (D) Relative distribution of the normal and flattened stage V LRP. D, dome-shaped primordia (black); F, flattened primordia (grey) (n = 15–48).

Mentions: Finally, the authors investigated whether the phenotype caused by newly discovered bioactive peptides may be an indication of their plausible function. Cleared roots were analysed after treatment with Pep f919-2, which is the strongest inhibitor of root branching in this study (Fig. 6), and compared with untreated roots or roots treated with a randomized Pep f919-2 (Fig. 7). This experiment confirmed that Pep f919-2 significantly decreased the number of emerged LRs. However, the peptide treatment did not affect the number of primordia being initiated (Fig. 7A). Instead, Pep f919-2-treated roots carried an unusually high number of primordia at stage V of development, which normally precedes the progression of the LR through the overlying cell layers (endodermis, cortex and epidermis) before it emerges from the body of the main root (Malamy and Benfey, 1997). Furthermore, the shape of the primordia was clearly different depending on the root treatment. Most primordia grew with a classical dome shape in the control plants (Fig. 7B). In contrast, in Pep f919-2-treated roots, the vast majority of LRPs appeared flattened as if pressed against the overlying tissues (Fig. 7C, D).


Expanding the repertoire of secretory peptides controlling root development with comparative genome analysis and functional assays.

Ghorbani S, Lin YC, Parizot B, Fernandez A, Njo MF, Van de Peer Y, Beeckman T, Hilson P - J. Exp. Bot. (2015)

LR-related phenotypes induced by the f919-2 peptide. (A) Distribution of LR developmental stages in roots 12 days after germination. I–VII, primordium stages; NE, non-emerged primordia; E, emerged LRs; total, total number of LRs; np, no peptide; r, randomized peptide. Results for one of two independent experiments are shown (see Materials and Methods). Error bars represent the 95% confidence interval. Asterisks indicate significant differences compared with the no-peptide control (*** P < 0.001). (B, C) Differential interference contrast images of representative stage V LRP-treated (C) or not treated (B) with the f919-2 peptide (10 μM). (D) Relative distribution of the normal and flattened stage V LRP. D, dome-shaped primordia (black); F, flattened primordia (grey) (n = 15–48).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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Figure 7: LR-related phenotypes induced by the f919-2 peptide. (A) Distribution of LR developmental stages in roots 12 days after germination. I–VII, primordium stages; NE, non-emerged primordia; E, emerged LRs; total, total number of LRs; np, no peptide; r, randomized peptide. Results for one of two independent experiments are shown (see Materials and Methods). Error bars represent the 95% confidence interval. Asterisks indicate significant differences compared with the no-peptide control (*** P < 0.001). (B, C) Differential interference contrast images of representative stage V LRP-treated (C) or not treated (B) with the f919-2 peptide (10 μM). (D) Relative distribution of the normal and flattened stage V LRP. D, dome-shaped primordia (black); F, flattened primordia (grey) (n = 15–48).
Mentions: Finally, the authors investigated whether the phenotype caused by newly discovered bioactive peptides may be an indication of their plausible function. Cleared roots were analysed after treatment with Pep f919-2, which is the strongest inhibitor of root branching in this study (Fig. 6), and compared with untreated roots or roots treated with a randomized Pep f919-2 (Fig. 7). This experiment confirmed that Pep f919-2 significantly decreased the number of emerged LRs. However, the peptide treatment did not affect the number of primordia being initiated (Fig. 7A). Instead, Pep f919-2-treated roots carried an unusually high number of primordia at stage V of development, which normally precedes the progression of the LR through the overlying cell layers (endodermis, cortex and epidermis) before it emerges from the body of the main root (Malamy and Benfey, 1997). Furthermore, the shape of the primordia was clearly different depending on the root treatment. Most primordia grew with a classical dome shape in the control plants (Fig. 7B). In contrast, in Pep f919-2-treated roots, the vast majority of LRPs appeared flattened as if pressed against the overlying tissues (Fig. 7C, D).

Bottom Line: Based on structural features that characterize SSP families known to take part in postembryonic development, this comparative genome analysis resulted in the identification of genes coding for oligopeptides potentially involved in cell-to-cell communication.The strategy used in the study, combining comparative genomics, transcriptome meta-analysis and peptide functional assays in planta, pinpoints factors potentially involved in non-cell-autonomous regulatory mechanisms.A similar approach can be implemented in different species for the study of a wide range of developmental programmes.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.

No MeSH data available.