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Expanding the repertoire of secretory peptides controlling root development with comparative genome analysis and functional assays.

Ghorbani S, Lin YC, Parizot B, Fernandez A, Njo MF, Van de Peer Y, Beeckman T, Hilson P - J. Exp. Bot. (2015)

Bottom Line: Based on structural features that characterize SSP families known to take part in postembryonic development, this comparative genome analysis resulted in the identification of genes coding for oligopeptides potentially involved in cell-to-cell communication.The strategy used in the study, combining comparative genomics, transcriptome meta-analysis and peptide functional assays in planta, pinpoints factors potentially involved in non-cell-autonomous regulatory mechanisms.A similar approach can be implemented in different species for the study of a wide range of developmental programmes.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.

No MeSH data available.


Root-related phenotypes induced by the identified SSPs. (A) Number of emerged LRs per unit length (mm) (n = 20–37). (B) Primary root length (n = 19–44). Seedlings (10 days after germination) were compared with controls after treatment with the indicated peptides. Error bars represent the 95% confidence interval. Asterisks mark significant differences: * P < 0.05; ** P < 0.005, *** P < 0.001. Data were pooled from independent biological replicates. (C) Induction of SSP gene transcription by auxin. Seedlings were treated with 1 µM NAA for the indicated time points. Fold changes were measured after qRT-PCR analysis of root tissues. Data are shown for one of two independent experiments. np, no peptide.
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Figure 6: Root-related phenotypes induced by the identified SSPs. (A) Number of emerged LRs per unit length (mm) (n = 20–37). (B) Primary root length (n = 19–44). Seedlings (10 days after germination) were compared with controls after treatment with the indicated peptides. Error bars represent the 95% confidence interval. Asterisks mark significant differences: * P < 0.05; ** P < 0.005, *** P < 0.001. Data were pooled from independent biological replicates. (C) Induction of SSP gene transcription by auxin. Seedlings were treated with 1 µM NAA for the indicated time points. Fold changes were measured after qRT-PCR analysis of root tissues. Data are shown for one of two independent experiments. np, no peptide.

Mentions: The number of LRs and the primary root length were compared between control seedlings and seedlings treated with 1 µM or 10 µM of peptides for three uncharacterized families. Peptides (Pep) from families f31 and f919 decreased the number of emerged LRs. Pep f919-2 (At4G34600), in particular, resulted in a 70% decrease compared with control untreated seedlings (Fig. 6A; Supplementary Fig. 4). In all cases, the effect was stronger or only detectable at 10 µM. Furthermore, plantlets treated with 10 µM of Pep f31-2 (At4G37295) were pale and arrested in growth. From the family f1528, only Pep f1528-2-2 (At2G23270) and Pep f1528-3-2 (At4G37290) induced significant differences compared with control untreated plants (Fig. 6A; Supplementary Fig. 4). Peptides inhibiting LR emergence had no detectable effect on primary root growth, except Pep f31-1 and Pep f919-2 and, at high concentration, Pep f919-1 and Pep f1528-2-1 (Fig. 6B; Supplementary Fig. 4). As expected, treatment with randomized Pep f31-2 and Pep f919-2 showed no effect on either root growth or LR emergence.


Expanding the repertoire of secretory peptides controlling root development with comparative genome analysis and functional assays.

Ghorbani S, Lin YC, Parizot B, Fernandez A, Njo MF, Van de Peer Y, Beeckman T, Hilson P - J. Exp. Bot. (2015)

Root-related phenotypes induced by the identified SSPs. (A) Number of emerged LRs per unit length (mm) (n = 20–37). (B) Primary root length (n = 19–44). Seedlings (10 days after germination) were compared with controls after treatment with the indicated peptides. Error bars represent the 95% confidence interval. Asterisks mark significant differences: * P < 0.05; ** P < 0.005, *** P < 0.001. Data were pooled from independent biological replicates. (C) Induction of SSP gene transcription by auxin. Seedlings were treated with 1 µM NAA for the indicated time points. Fold changes were measured after qRT-PCR analysis of root tissues. Data are shown for one of two independent experiments. np, no peptide.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
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Figure 6: Root-related phenotypes induced by the identified SSPs. (A) Number of emerged LRs per unit length (mm) (n = 20–37). (B) Primary root length (n = 19–44). Seedlings (10 days after germination) were compared with controls after treatment with the indicated peptides. Error bars represent the 95% confidence interval. Asterisks mark significant differences: * P < 0.05; ** P < 0.005, *** P < 0.001. Data were pooled from independent biological replicates. (C) Induction of SSP gene transcription by auxin. Seedlings were treated with 1 µM NAA for the indicated time points. Fold changes were measured after qRT-PCR analysis of root tissues. Data are shown for one of two independent experiments. np, no peptide.
Mentions: The number of LRs and the primary root length were compared between control seedlings and seedlings treated with 1 µM or 10 µM of peptides for three uncharacterized families. Peptides (Pep) from families f31 and f919 decreased the number of emerged LRs. Pep f919-2 (At4G34600), in particular, resulted in a 70% decrease compared with control untreated seedlings (Fig. 6A; Supplementary Fig. 4). In all cases, the effect was stronger or only detectable at 10 µM. Furthermore, plantlets treated with 10 µM of Pep f31-2 (At4G37295) were pale and arrested in growth. From the family f1528, only Pep f1528-2-2 (At2G23270) and Pep f1528-3-2 (At4G37290) induced significant differences compared with control untreated plants (Fig. 6A; Supplementary Fig. 4). Peptides inhibiting LR emergence had no detectable effect on primary root growth, except Pep f31-1 and Pep f919-2 and, at high concentration, Pep f919-1 and Pep f1528-2-1 (Fig. 6B; Supplementary Fig. 4). As expected, treatment with randomized Pep f31-2 and Pep f919-2 showed no effect on either root growth or LR emergence.

Bottom Line: Based on structural features that characterize SSP families known to take part in postembryonic development, this comparative genome analysis resulted in the identification of genes coding for oligopeptides potentially involved in cell-to-cell communication.The strategy used in the study, combining comparative genomics, transcriptome meta-analysis and peptide functional assays in planta, pinpoints factors potentially involved in non-cell-autonomous regulatory mechanisms.A similar approach can be implemented in different species for the study of a wide range of developmental programmes.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.

No MeSH data available.