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Expanding the repertoire of secretory peptides controlling root development with comparative genome analysis and functional assays.

Ghorbani S, Lin YC, Parizot B, Fernandez A, Njo MF, Van de Peer Y, Beeckman T, Hilson P - J. Exp. Bot. (2015)

Bottom Line: Based on structural features that characterize SSP families known to take part in postembryonic development, this comparative genome analysis resulted in the identification of genes coding for oligopeptides potentially involved in cell-to-cell communication.The strategy used in the study, combining comparative genomics, transcriptome meta-analysis and peptide functional assays in planta, pinpoints factors potentially involved in non-cell-autonomous regulatory mechanisms.A similar approach can be implemented in different species for the study of a wide range of developmental programmes.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.

No MeSH data available.


Primary sequence alignment of Arabidopsis SSPs tested in root development assays. The multiple sequence alignment was generated with ClustalW2. f# refers to SSP families as defined in Supplementary Table 2.
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Figure 5: Primary sequence alignment of Arabidopsis SSPs tested in root development assays. The multiple sequence alignment was generated with ClustalW2. f# refers to SSP families as defined in Supplementary Table 2.

Mentions: To investigate the potential role of uncharacterized SSPs, seedlings were grown on agar medium supplemented with synthetic peptides corresponding to conserved C-terminal stretches (Fig. 5; Supplementary Table 5). Whereas synthetic SSPs, including members of the CLV3/CLE and GLV/RGF/CLEL families, are active at nanomolar concentrations (Murphy et al., 2012), the absence of certain post-translational modifications in synthetic copies has been shown to reduce bioactivity compared with native peptides (Matsubayashi, 2014; Seitz, 2000; Shinohara and Matsubayashi, 2013). To avoid false-negative results due to lack of post-translational modification, micromolar concentrations of synthetic peptides were applied, as is commonly reported in such experiments.


Expanding the repertoire of secretory peptides controlling root development with comparative genome analysis and functional assays.

Ghorbani S, Lin YC, Parizot B, Fernandez A, Njo MF, Van de Peer Y, Beeckman T, Hilson P - J. Exp. Bot. (2015)

Primary sequence alignment of Arabidopsis SSPs tested in root development assays. The multiple sequence alignment was generated with ClustalW2. f# refers to SSP families as defined in Supplementary Table 2.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526923&req=5

Figure 5: Primary sequence alignment of Arabidopsis SSPs tested in root development assays. The multiple sequence alignment was generated with ClustalW2. f# refers to SSP families as defined in Supplementary Table 2.
Mentions: To investigate the potential role of uncharacterized SSPs, seedlings were grown on agar medium supplemented with synthetic peptides corresponding to conserved C-terminal stretches (Fig. 5; Supplementary Table 5). Whereas synthetic SSPs, including members of the CLV3/CLE and GLV/RGF/CLEL families, are active at nanomolar concentrations (Murphy et al., 2012), the absence of certain post-translational modifications in synthetic copies has been shown to reduce bioactivity compared with native peptides (Matsubayashi, 2014; Seitz, 2000; Shinohara and Matsubayashi, 2013). To avoid false-negative results due to lack of post-translational modification, micromolar concentrations of synthetic peptides were applied, as is commonly reported in such experiments.

Bottom Line: Based on structural features that characterize SSP families known to take part in postembryonic development, this comparative genome analysis resulted in the identification of genes coding for oligopeptides potentially involved in cell-to-cell communication.The strategy used in the study, combining comparative genomics, transcriptome meta-analysis and peptide functional assays in planta, pinpoints factors potentially involved in non-cell-autonomous regulatory mechanisms.A similar approach can be implemented in different species for the study of a wide range of developmental programmes.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.

No MeSH data available.