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The GLV6/RGF8/CLEL2 peptide regulates early pericycle divisions during lateral root initiation.

Fernandez A, Drozdzecki A, Hoogewijs K, Vassileva V, Madder A, Beeckman T, Hilson P - J. Exp. Bot. (2015)

Bottom Line: Small peptides of the Arabidopsis GLV/RGF/CLEL family are involved in different developmental programmes, including meristem maintenance and gravitropic responses.In addition, our previous report suggested that they also participate in the formation of lateral roots.Specifically, GLV6 is transcribed during the first stages of primordium development and GLV6 overexpression results in a strong reduction of emerged lateral roots.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium. Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium.

No MeSH data available.


Related in: MedlinePlus

GLV6 peptide treatment affects nuclear migration before the first division of the founder cells. (A) Stage II primordium is observed 15h after mechanical bending of the GATA23pro:NLS-GFP-GUS primary root. (B) Dynamics of LRFC nuclei represented as the distance between the two nuclei over time, from the beginning of the experiment until the first anticlinal division. Three independent roots are shown. Arrows show timing examples of the indicated events. Higher magnification of the area framed in A shows different events taking place in LRFCs during primordium initiation: (C) immediately after bending; (D) after nuclear migration; (E) first anticlinal division; (F) second anticlinal division; (G, H) first periclinal division in central nuclei; (I) second periclinal division in flanking nuclei. Notice plane change again in central nuclei. (J) Effect of peptide treatment on the onset of nuclear migration. The distance between LRFC nuclei was measured from t0 up to the first anticlinal division in the presence of 1 µM of the indicated peptides. Three independent roots are shown. (K) Representative images showing beginning and end of nuclear migration and first anticlinal division in LRFCs treated with 1 µM of the indicated peptides. (L) Quantification of events illustrated in K. Charts show average time/distance ±SEM (n=6 to 8). Time is shown as h:min. All indicated time points refer to time after the first image was taken. Nuclei were followed in six to eight movies for each condition. Scale bars: 50 µm (A); 20 µm (C−I and K).
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Figure 6: GLV6 peptide treatment affects nuclear migration before the first division of the founder cells. (A) Stage II primordium is observed 15h after mechanical bending of the GATA23pro:NLS-GFP-GUS primary root. (B) Dynamics of LRFC nuclei represented as the distance between the two nuclei over time, from the beginning of the experiment until the first anticlinal division. Three independent roots are shown. Arrows show timing examples of the indicated events. Higher magnification of the area framed in A shows different events taking place in LRFCs during primordium initiation: (C) immediately after bending; (D) after nuclear migration; (E) first anticlinal division; (F) second anticlinal division; (G, H) first periclinal division in central nuclei; (I) second periclinal division in flanking nuclei. Notice plane change again in central nuclei. (J) Effect of peptide treatment on the onset of nuclear migration. The distance between LRFC nuclei was measured from t0 up to the first anticlinal division in the presence of 1 µM of the indicated peptides. Three independent roots are shown. (K) Representative images showing beginning and end of nuclear migration and first anticlinal division in LRFCs treated with 1 µM of the indicated peptides. (L) Quantification of events illustrated in K. Charts show average time/distance ±SEM (n=6 to 8). Time is shown as h:min. All indicated time points refer to time after the first image was taken. Nuclei were followed in six to eight movies for each condition. Scale bars: 50 µm (A); 20 µm (C−I and K).

Mentions: To determine how early GLV6 may function, it is necessary to detect the very first cellular events at the onset of LRP initiation. For this purpose, we used the GATA23 gene whose transcription is induced when founder cells are specified in the elongation zone. Therefore, in a GATA23pro:NLS-GFP-GUS maker line, the founder cell nuclei can already be identified before the first cellular signs of LPR initiation as marked with a GFP signal (De Rybel et al., 2010). As reported before, primordium formation can be induced by mechanical bending of the primary root (Ditengou et al., 2008; Laskowski et al., 2008). Thus, we manually bent the main root of GATA23pro:NLS-GFP-GUS seedlings and performed confocal time-lapse series for 14−16h, long enough for the appearance of a stage II primodium in the bent region (Fig. 6A).


The GLV6/RGF8/CLEL2 peptide regulates early pericycle divisions during lateral root initiation.

Fernandez A, Drozdzecki A, Hoogewijs K, Vassileva V, Madder A, Beeckman T, Hilson P - J. Exp. Bot. (2015)

GLV6 peptide treatment affects nuclear migration before the first division of the founder cells. (A) Stage II primordium is observed 15h after mechanical bending of the GATA23pro:NLS-GFP-GUS primary root. (B) Dynamics of LRFC nuclei represented as the distance between the two nuclei over time, from the beginning of the experiment until the first anticlinal division. Three independent roots are shown. Arrows show timing examples of the indicated events. Higher magnification of the area framed in A shows different events taking place in LRFCs during primordium initiation: (C) immediately after bending; (D) after nuclear migration; (E) first anticlinal division; (F) second anticlinal division; (G, H) first periclinal division in central nuclei; (I) second periclinal division in flanking nuclei. Notice plane change again in central nuclei. (J) Effect of peptide treatment on the onset of nuclear migration. The distance between LRFC nuclei was measured from t0 up to the first anticlinal division in the presence of 1 µM of the indicated peptides. Three independent roots are shown. (K) Representative images showing beginning and end of nuclear migration and first anticlinal division in LRFCs treated with 1 µM of the indicated peptides. (L) Quantification of events illustrated in K. Charts show average time/distance ±SEM (n=6 to 8). Time is shown as h:min. All indicated time points refer to time after the first image was taken. Nuclei were followed in six to eight movies for each condition. Scale bars: 50 µm (A); 20 µm (C−I and K).
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Figure 6: GLV6 peptide treatment affects nuclear migration before the first division of the founder cells. (A) Stage II primordium is observed 15h after mechanical bending of the GATA23pro:NLS-GFP-GUS primary root. (B) Dynamics of LRFC nuclei represented as the distance between the two nuclei over time, from the beginning of the experiment until the first anticlinal division. Three independent roots are shown. Arrows show timing examples of the indicated events. Higher magnification of the area framed in A shows different events taking place in LRFCs during primordium initiation: (C) immediately after bending; (D) after nuclear migration; (E) first anticlinal division; (F) second anticlinal division; (G, H) first periclinal division in central nuclei; (I) second periclinal division in flanking nuclei. Notice plane change again in central nuclei. (J) Effect of peptide treatment on the onset of nuclear migration. The distance between LRFC nuclei was measured from t0 up to the first anticlinal division in the presence of 1 µM of the indicated peptides. Three independent roots are shown. (K) Representative images showing beginning and end of nuclear migration and first anticlinal division in LRFCs treated with 1 µM of the indicated peptides. (L) Quantification of events illustrated in K. Charts show average time/distance ±SEM (n=6 to 8). Time is shown as h:min. All indicated time points refer to time after the first image was taken. Nuclei were followed in six to eight movies for each condition. Scale bars: 50 µm (A); 20 µm (C−I and K).
Mentions: To determine how early GLV6 may function, it is necessary to detect the very first cellular events at the onset of LRP initiation. For this purpose, we used the GATA23 gene whose transcription is induced when founder cells are specified in the elongation zone. Therefore, in a GATA23pro:NLS-GFP-GUS maker line, the founder cell nuclei can already be identified before the first cellular signs of LPR initiation as marked with a GFP signal (De Rybel et al., 2010). As reported before, primordium formation can be induced by mechanical bending of the primary root (Ditengou et al., 2008; Laskowski et al., 2008). Thus, we manually bent the main root of GATA23pro:NLS-GFP-GUS seedlings and performed confocal time-lapse series for 14−16h, long enough for the appearance of a stage II primodium in the bent region (Fig. 6A).

Bottom Line: Small peptides of the Arabidopsis GLV/RGF/CLEL family are involved in different developmental programmes, including meristem maintenance and gravitropic responses.In addition, our previous report suggested that they also participate in the formation of lateral roots.Specifically, GLV6 is transcribed during the first stages of primordium development and GLV6 overexpression results in a strong reduction of emerged lateral roots.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium. Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium.

No MeSH data available.


Related in: MedlinePlus