Limits...
The GLV6/RGF8/CLEL2 peptide regulates early pericycle divisions during lateral root initiation.

Fernandez A, Drozdzecki A, Hoogewijs K, Vassileva V, Madder A, Beeckman T, Hilson P - J. Exp. Bot. (2015)

Bottom Line: Small peptides of the Arabidopsis GLV/RGF/CLEL family are involved in different developmental programmes, including meristem maintenance and gravitropic responses.In addition, our previous report suggested that they also participate in the formation of lateral roots.Specifically, GLV6 is transcribed during the first stages of primordium development and GLV6 overexpression results in a strong reduction of emerged lateral roots.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium. Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium.

No MeSH data available.


Related in: MedlinePlus

Excess GLV6 activity disrupts the first pericycle asymmetric divisions during primordium initiation. (A−E) The GFP signal in PIN1pro:PIN1-GFP plants was used as a plasma membrane marker. These plants were either crossed to GLV6 overexpression plants (A, B) and the F1 progeny was analysed or they were germinated on 1 µM of the indicated peptide (C−E). Arrowheads indicate anticlinal divisions. Scale bars, 20 µm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4526922&req=5

Figure 5: Excess GLV6 activity disrupts the first pericycle asymmetric divisions during primordium initiation. (A−E) The GFP signal in PIN1pro:PIN1-GFP plants was used as a plasma membrane marker. These plants were either crossed to GLV6 overexpression plants (A, B) and the F1 progeny was analysed or they were germinated on 1 µM of the indicated peptide (C−E). Arrowheads indicate anticlinal divisions. Scale bars, 20 µm.

Mentions: To further study GLV6 function in primordium initiation we used a PIN1pro:PIN1-GFP line as a plasma membrane marker to visualize the first divisions occurring in LRFCs since this auxin transporter is known to localize to the plasma membrane in the forming primordium (Benkova et al., 2003). In untreated PIN1pro:PIN1-GFP plants and in plants treated with a random control peptide, the first asymmetric anticlinal division was clearly visible, resulting in two small central cells and two larger flanking cells as previously reported (Fig. 5A, C). However, a remarkably different pattern was observed when these plants were either crossed to 35Spro:GLV6 (Fig. 5B) or germinated on GLV6p SO3 (Fig. 5D, E), whereby the initial anticlinal divisions yielded daughter cells of more similar sizes. Sometimes, one of the two neighbouring LRFCs divided symmetrically while the other one divided asymmetrically (Fig. 5D).


The GLV6/RGF8/CLEL2 peptide regulates early pericycle divisions during lateral root initiation.

Fernandez A, Drozdzecki A, Hoogewijs K, Vassileva V, Madder A, Beeckman T, Hilson P - J. Exp. Bot. (2015)

Excess GLV6 activity disrupts the first pericycle asymmetric divisions during primordium initiation. (A−E) The GFP signal in PIN1pro:PIN1-GFP plants was used as a plasma membrane marker. These plants were either crossed to GLV6 overexpression plants (A, B) and the F1 progeny was analysed or they were germinated on 1 µM of the indicated peptide (C−E). Arrowheads indicate anticlinal divisions. Scale bars, 20 µm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526922&req=5

Figure 5: Excess GLV6 activity disrupts the first pericycle asymmetric divisions during primordium initiation. (A−E) The GFP signal in PIN1pro:PIN1-GFP plants was used as a plasma membrane marker. These plants were either crossed to GLV6 overexpression plants (A, B) and the F1 progeny was analysed or they were germinated on 1 µM of the indicated peptide (C−E). Arrowheads indicate anticlinal divisions. Scale bars, 20 µm.
Mentions: To further study GLV6 function in primordium initiation we used a PIN1pro:PIN1-GFP line as a plasma membrane marker to visualize the first divisions occurring in LRFCs since this auxin transporter is known to localize to the plasma membrane in the forming primordium (Benkova et al., 2003). In untreated PIN1pro:PIN1-GFP plants and in plants treated with a random control peptide, the first asymmetric anticlinal division was clearly visible, resulting in two small central cells and two larger flanking cells as previously reported (Fig. 5A, C). However, a remarkably different pattern was observed when these plants were either crossed to 35Spro:GLV6 (Fig. 5B) or germinated on GLV6p SO3 (Fig. 5D, E), whereby the initial anticlinal divisions yielded daughter cells of more similar sizes. Sometimes, one of the two neighbouring LRFCs divided symmetrically while the other one divided asymmetrically (Fig. 5D).

Bottom Line: Small peptides of the Arabidopsis GLV/RGF/CLEL family are involved in different developmental programmes, including meristem maintenance and gravitropic responses.In addition, our previous report suggested that they also participate in the formation of lateral roots.Specifically, GLV6 is transcribed during the first stages of primordium development and GLV6 overexpression results in a strong reduction of emerged lateral roots.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium. Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium.

No MeSH data available.


Related in: MedlinePlus