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The GLV6/RGF8/CLEL2 peptide regulates early pericycle divisions during lateral root initiation.

Fernandez A, Drozdzecki A, Hoogewijs K, Vassileva V, Madder A, Beeckman T, Hilson P - J. Exp. Bot. (2015)

Bottom Line: Small peptides of the Arabidopsis GLV/RGF/CLEL family are involved in different developmental programmes, including meristem maintenance and gravitropic responses.In addition, our previous report suggested that they also participate in the formation of lateral roots.Specifically, GLV6 is transcribed during the first stages of primordium development and GLV6 overexpression results in a strong reduction of emerged lateral roots.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium. Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium.

No MeSH data available.


Related in: MedlinePlus

Treatments with GLV6 peptides phenocopy overexpression of the gene. (A) ELR density of seedlings treated with GLV6 synthetic peptides. The asterisks indicate statistically significant differences compared to the randomized rGLV6p SO3 control at the same concentration (*,P<0.05; **, P<0.01; ***, P<0.001) (n=25). (B) Treatment with GLV6 bioactive peptides induce pericycle divisions similar to those observed in GLV6 overexpression lines. Peptides were added at 1 µM. Arrowheads indicate anticlinal divisions. Scale bars, 50 µm.
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Figure 4: Treatments with GLV6 peptides phenocopy overexpression of the gene. (A) ELR density of seedlings treated with GLV6 synthetic peptides. The asterisks indicate statistically significant differences compared to the randomized rGLV6p SO3 control at the same concentration (*,P<0.05; **, P<0.01; ***, P<0.001) (n=25). (B) Treatment with GLV6 bioactive peptides induce pericycle divisions similar to those observed in GLV6 overexpression lines. Peptides were added at 1 µM. Arrowheads indicate anticlinal divisions. Scale bars, 50 µm.

Mentions: We then investigated whether LR development can be perturbed upon treatment with a synthetic sulfonated GLV6 peptide (GLV6p SO3). A random peptide (rGLV6p SO3) containing the same amino acids but in a randomized sequence was used as control. Seedlings germinated in liquid media supplemented with GLV6p SO3 had decreased ELR density compared to untreated plants or rGLV6p SO3 controls reminiscent of the phenotype observed in GLV6 overexpression plants (Fig. 4A). Nevertheless, the reduction in emerged LR density resulting from peptide treatment, even at the highest tested concentration (4 µM), was not as strong as that of GLV6 ectopic expression (compare Fig. 2G and 4A). Since the functional role of proline hydroxylation has not been reported so far for GLV peptides, we then tested whether it could further increase peptide bioactivity. Treatments with GLV6p Hyp SO3 had the same effect as those with GLV6p SO3, indicating that, at least in exogenous synthetic GLV6 peptides, the presence of a hydroxproline residue does not enhance bioactivity (Fig. 4A). As in 35Spro:GLV6 plants, excessive anticlinal divisions were observed in early stage LRPs of roots germinated in the presence of GLV6 SO3 or GLV6p Hyp SO3 peptides (Fig. 4B and data not shown).


The GLV6/RGF8/CLEL2 peptide regulates early pericycle divisions during lateral root initiation.

Fernandez A, Drozdzecki A, Hoogewijs K, Vassileva V, Madder A, Beeckman T, Hilson P - J. Exp. Bot. (2015)

Treatments with GLV6 peptides phenocopy overexpression of the gene. (A) ELR density of seedlings treated with GLV6 synthetic peptides. The asterisks indicate statistically significant differences compared to the randomized rGLV6p SO3 control at the same concentration (*,P<0.05; **, P<0.01; ***, P<0.001) (n=25). (B) Treatment with GLV6 bioactive peptides induce pericycle divisions similar to those observed in GLV6 overexpression lines. Peptides were added at 1 µM. Arrowheads indicate anticlinal divisions. Scale bars, 50 µm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526922&req=5

Figure 4: Treatments with GLV6 peptides phenocopy overexpression of the gene. (A) ELR density of seedlings treated with GLV6 synthetic peptides. The asterisks indicate statistically significant differences compared to the randomized rGLV6p SO3 control at the same concentration (*,P<0.05; **, P<0.01; ***, P<0.001) (n=25). (B) Treatment with GLV6 bioactive peptides induce pericycle divisions similar to those observed in GLV6 overexpression lines. Peptides were added at 1 µM. Arrowheads indicate anticlinal divisions. Scale bars, 50 µm.
Mentions: We then investigated whether LR development can be perturbed upon treatment with a synthetic sulfonated GLV6 peptide (GLV6p SO3). A random peptide (rGLV6p SO3) containing the same amino acids but in a randomized sequence was used as control. Seedlings germinated in liquid media supplemented with GLV6p SO3 had decreased ELR density compared to untreated plants or rGLV6p SO3 controls reminiscent of the phenotype observed in GLV6 overexpression plants (Fig. 4A). Nevertheless, the reduction in emerged LR density resulting from peptide treatment, even at the highest tested concentration (4 µM), was not as strong as that of GLV6 ectopic expression (compare Fig. 2G and 4A). Since the functional role of proline hydroxylation has not been reported so far for GLV peptides, we then tested whether it could further increase peptide bioactivity. Treatments with GLV6p Hyp SO3 had the same effect as those with GLV6p SO3, indicating that, at least in exogenous synthetic GLV6 peptides, the presence of a hydroxproline residue does not enhance bioactivity (Fig. 4A). As in 35Spro:GLV6 plants, excessive anticlinal divisions were observed in early stage LRPs of roots germinated in the presence of GLV6 SO3 or GLV6p Hyp SO3 peptides (Fig. 4B and data not shown).

Bottom Line: Small peptides of the Arabidopsis GLV/RGF/CLEL family are involved in different developmental programmes, including meristem maintenance and gravitropic responses.In addition, our previous report suggested that they also participate in the formation of lateral roots.Specifically, GLV6 is transcribed during the first stages of primordium development and GLV6 overexpression results in a strong reduction of emerged lateral roots.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium. Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium.

No MeSH data available.


Related in: MedlinePlus