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The GLV6/RGF8/CLEL2 peptide regulates early pericycle divisions during lateral root initiation.

Fernandez A, Drozdzecki A, Hoogewijs K, Vassileva V, Madder A, Beeckman T, Hilson P - J. Exp. Bot. (2015)

Bottom Line: Small peptides of the Arabidopsis GLV/RGF/CLEL family are involved in different developmental programmes, including meristem maintenance and gravitropic responses.In addition, our previous report suggested that they also participate in the formation of lateral roots.Specifically, GLV6 is transcribed during the first stages of primordium development and GLV6 overexpression results in a strong reduction of emerged lateral roots.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium. Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium.

No MeSH data available.


Related in: MedlinePlus

The GLV6 mature peptide is contained in the C-terminus of the precursor. Figure shows the ELR density in lines overexpressing truncated GLV6 ORFs (n=12−28). SP, signal peptide; GLV, conserved C-terminal domain; VR, variable region. The asterisks indicate that the ELR density was significantly different compared to the empty vector control (**, P<0.01; ***, P<0.001).
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Figure 3: The GLV6 mature peptide is contained in the C-terminus of the precursor. Figure shows the ELR density in lines overexpressing truncated GLV6 ORFs (n=12−28). SP, signal peptide; GLV, conserved C-terminal domain; VR, variable region. The asterisks indicate that the ELR density was significantly different compared to the empty vector control (**, P<0.01; ***, P<0.001).

Mentions: Signalling peptides are translated as large precursors that are processed to yield the mature secreted peptides (Matsubayashi, 2011). Three domains can be distinguished in GLV precursor proteins, from the amino- to the carboxyl-terminus: a signal peptide (SP) presumably necessary for secretion, a variable region (VR), and a conserved GLV motif that defines the family (Fernandez et al., 2013b). To confirm that the bioactive GLV6 signal peptide is encoded in the GLV motif, we overexpressed truncated versions of the GLV6 ORF in Arabidopsis plants. The tested constructs included the GLV6 sequences either (i) coding for the full precursor where the three aforementioned domains are present, (ii) lacking the region corresponding to the SP (35Spro:GLV6ΔSP), (iii) lacking the region corresponding to the GLV motif (35Spro:GLV6ΔGLV), or (iv) comprising a translational fusion between the SP and the GLV domains and thus where the variable region has been deleted (35Spro:GLV6ΔVR) (Fig. 3).


The GLV6/RGF8/CLEL2 peptide regulates early pericycle divisions during lateral root initiation.

Fernandez A, Drozdzecki A, Hoogewijs K, Vassileva V, Madder A, Beeckman T, Hilson P - J. Exp. Bot. (2015)

The GLV6 mature peptide is contained in the C-terminus of the precursor. Figure shows the ELR density in lines overexpressing truncated GLV6 ORFs (n=12−28). SP, signal peptide; GLV, conserved C-terminal domain; VR, variable region. The asterisks indicate that the ELR density was significantly different compared to the empty vector control (**, P<0.01; ***, P<0.001).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526922&req=5

Figure 3: The GLV6 mature peptide is contained in the C-terminus of the precursor. Figure shows the ELR density in lines overexpressing truncated GLV6 ORFs (n=12−28). SP, signal peptide; GLV, conserved C-terminal domain; VR, variable region. The asterisks indicate that the ELR density was significantly different compared to the empty vector control (**, P<0.01; ***, P<0.001).
Mentions: Signalling peptides are translated as large precursors that are processed to yield the mature secreted peptides (Matsubayashi, 2011). Three domains can be distinguished in GLV precursor proteins, from the amino- to the carboxyl-terminus: a signal peptide (SP) presumably necessary for secretion, a variable region (VR), and a conserved GLV motif that defines the family (Fernandez et al., 2013b). To confirm that the bioactive GLV6 signal peptide is encoded in the GLV motif, we overexpressed truncated versions of the GLV6 ORF in Arabidopsis plants. The tested constructs included the GLV6 sequences either (i) coding for the full precursor where the three aforementioned domains are present, (ii) lacking the region corresponding to the SP (35Spro:GLV6ΔSP), (iii) lacking the region corresponding to the GLV motif (35Spro:GLV6ΔGLV), or (iv) comprising a translational fusion between the SP and the GLV domains and thus where the variable region has been deleted (35Spro:GLV6ΔVR) (Fig. 3).

Bottom Line: Small peptides of the Arabidopsis GLV/RGF/CLEL family are involved in different developmental programmes, including meristem maintenance and gravitropic responses.In addition, our previous report suggested that they also participate in the formation of lateral roots.Specifically, GLV6 is transcribed during the first stages of primordium development and GLV6 overexpression results in a strong reduction of emerged lateral roots.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium. Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium.

No MeSH data available.


Related in: MedlinePlus