Limits...
The GLV6/RGF8/CLEL2 peptide regulates early pericycle divisions during lateral root initiation.

Fernandez A, Drozdzecki A, Hoogewijs K, Vassileva V, Madder A, Beeckman T, Hilson P - J. Exp. Bot. (2015)

Bottom Line: Small peptides of the Arabidopsis GLV/RGF/CLEL family are involved in different developmental programmes, including meristem maintenance and gravitropic responses.In addition, our previous report suggested that they also participate in the formation of lateral roots.Specifically, GLV6 is transcribed during the first stages of primordium development and GLV6 overexpression results in a strong reduction of emerged lateral roots.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium. Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium.

No MeSH data available.


Related in: MedlinePlus

GLV6 expression pattern during lateral root initiation. GLV6 transcription was detected in a GLV6pro:NLS-GFP-GUS line. (A) GLV6 transcription in the primary root. Cell walls were counterstained with propidium iodide (red). (B−G) GLV6 expression at the site of lateral root initiation in plants carrying the GLV6pro:NLS-GFP-GUS marker together with the Wave 131Y plasma membrane reporter. (B) The GLV6 signal is not detected in pericycle cells at the beginning of the differentiation zone. (C) The GLV6pro-driven GFP nuclear signal appears in founder cells prior to the first anticlinal division. (D−G) GLV6 transcription remains active throughout primordium formation and is confined to the central cells at later stages. The arrowheads point to expression in the endodermal cell adjacent to the primordium. Scale bars, 20 µm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4526922&req=5

Figure 1: GLV6 expression pattern during lateral root initiation. GLV6 transcription was detected in a GLV6pro:NLS-GFP-GUS line. (A) GLV6 transcription in the primary root. Cell walls were counterstained with propidium iodide (red). (B−G) GLV6 expression at the site of lateral root initiation in plants carrying the GLV6pro:NLS-GFP-GUS marker together with the Wave 131Y plasma membrane reporter. (B) The GLV6 signal is not detected in pericycle cells at the beginning of the differentiation zone. (C) The GLV6pro-driven GFP nuclear signal appears in founder cells prior to the first anticlinal division. (D−G) GLV6 transcription remains active throughout primordium formation and is confined to the central cells at later stages. The arrowheads point to expression in the endodermal cell adjacent to the primordium. Scale bars, 20 µm.

Mentions: To address this hypothesis, we first examined the timing of GLV6 induction during primordium initiation in plants carrying the GLV6pro::NLS-GFP-GUS transgene, where a nuclear GFP signal indicates GLV6 transcription (Fig. 1). To visualize cell boundaries, we introduced in these plants a plasma membrane marker (Wave 131Y; Geldner et al., 2009) (Fig. 1B−G). Analysis of the reporter line in the maturation zone revealed that GLV6 is only transcribed in pericycle cells where a primordium is forming and appeared also at later stages in the endodermal cell(s) surrounding the primordium. The GFP nuclear signal was first detected in paired pericycle cells with nuclei positioned close to the common cell wall suggesting that these are LRFCs (Fig. 1C), then after the first division in all primordium stages (Fig. 1D−G). At later stages, GLV6 transcription was mainly restricted to the centre of the developing primordium (Fig. 1F, G).


The GLV6/RGF8/CLEL2 peptide regulates early pericycle divisions during lateral root initiation.

Fernandez A, Drozdzecki A, Hoogewijs K, Vassileva V, Madder A, Beeckman T, Hilson P - J. Exp. Bot. (2015)

GLV6 expression pattern during lateral root initiation. GLV6 transcription was detected in a GLV6pro:NLS-GFP-GUS line. (A) GLV6 transcription in the primary root. Cell walls were counterstained with propidium iodide (red). (B−G) GLV6 expression at the site of lateral root initiation in plants carrying the GLV6pro:NLS-GFP-GUS marker together with the Wave 131Y plasma membrane reporter. (B) The GLV6 signal is not detected in pericycle cells at the beginning of the differentiation zone. (C) The GLV6pro-driven GFP nuclear signal appears in founder cells prior to the first anticlinal division. (D−G) GLV6 transcription remains active throughout primordium formation and is confined to the central cells at later stages. The arrowheads point to expression in the endodermal cell adjacent to the primordium. Scale bars, 20 µm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526922&req=5

Figure 1: GLV6 expression pattern during lateral root initiation. GLV6 transcription was detected in a GLV6pro:NLS-GFP-GUS line. (A) GLV6 transcription in the primary root. Cell walls were counterstained with propidium iodide (red). (B−G) GLV6 expression at the site of lateral root initiation in plants carrying the GLV6pro:NLS-GFP-GUS marker together with the Wave 131Y plasma membrane reporter. (B) The GLV6 signal is not detected in pericycle cells at the beginning of the differentiation zone. (C) The GLV6pro-driven GFP nuclear signal appears in founder cells prior to the first anticlinal division. (D−G) GLV6 transcription remains active throughout primordium formation and is confined to the central cells at later stages. The arrowheads point to expression in the endodermal cell adjacent to the primordium. Scale bars, 20 µm.
Mentions: To address this hypothesis, we first examined the timing of GLV6 induction during primordium initiation in plants carrying the GLV6pro::NLS-GFP-GUS transgene, where a nuclear GFP signal indicates GLV6 transcription (Fig. 1). To visualize cell boundaries, we introduced in these plants a plasma membrane marker (Wave 131Y; Geldner et al., 2009) (Fig. 1B−G). Analysis of the reporter line in the maturation zone revealed that GLV6 is only transcribed in pericycle cells where a primordium is forming and appeared also at later stages in the endodermal cell(s) surrounding the primordium. The GFP nuclear signal was first detected in paired pericycle cells with nuclei positioned close to the common cell wall suggesting that these are LRFCs (Fig. 1C), then after the first division in all primordium stages (Fig. 1D−G). At later stages, GLV6 transcription was mainly restricted to the centre of the developing primordium (Fig. 1F, G).

Bottom Line: Small peptides of the Arabidopsis GLV/RGF/CLEL family are involved in different developmental programmes, including meristem maintenance and gravitropic responses.In addition, our previous report suggested that they also participate in the formation of lateral roots.Specifically, GLV6 is transcribed during the first stages of primordium development and GLV6 overexpression results in a strong reduction of emerged lateral roots.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium. Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium.

No MeSH data available.


Related in: MedlinePlus