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CLE19 expressed in the embryo regulates both cotyledon establishment and endosperm development in Arabidopsis.

Xu TT, Ren SC, Song XF, Liu CM - J. Exp. Bot. (2015)

Bottom Line: CLE19 is expressed in the epidermal layers of the cotyledon primordia, hypocotyl, and root cap in the embryo.Transgenic plants carrying an antagonistic CLE19 G6T construct expressed under the control of CLE19 regulatory elements exhibited a dominant seed abortion phenotype, with defective cotyledon establishment in embryos and delayed nuclear proliferation and cellularization in endosperms.We therefore propose that CLE19 may act as a mobile peptide co-ordinating embryo and endosperm development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Fragrant Hill, Beijing 100093, China University of Chinese Academy of Sciences, Beijing 100049, China.

No MeSH data available.


Related in: MedlinePlus

Prolonged and elevated expression of early endosperm-specific genes in the endosperm of pCLE19:CLE19G6T:tCLE19 transgenic plants. (A) qRT-PCR analyses showed elevated expression of MEA, FIS2, and AGL62 in those abnormal ovules (a) from a pCLE19:CLE19G6T:tCLE19 transgenic plant (CLE19G6T), as compared with those normal ovules (n) from the same plant, and ovules from the wild type (WT). All ovules were analysed at 12 DAP. Data represent the mean ±SD of three independently extracted RNA samples. (B–E) GUS expression in seeds from the wild type (B) and pCLE19:CLE19G6T:tCLE19 transgenic plants carrying a pMEA:GUS reporter construct, examined at 12 DAP (C–E). Note that aborted seeds in (D) and (E) showed prolonged GUS expression in chalarzal endosperm (marked by asterisks), but not in the normal seed (C) in the same plant. Embryos are traced with dotted lines. c, cotyledon; hy, hypocotyl; h, heart-shaped stage embryo; t, torpedo-stage embryo. Asterisks indicate significant differences from the wild-type (P<0.01 by Student’s t-test). Scale bars in B–E=50 μm.
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Figure 7: Prolonged and elevated expression of early endosperm-specific genes in the endosperm of pCLE19:CLE19G6T:tCLE19 transgenic plants. (A) qRT-PCR analyses showed elevated expression of MEA, FIS2, and AGL62 in those abnormal ovules (a) from a pCLE19:CLE19G6T:tCLE19 transgenic plant (CLE19G6T), as compared with those normal ovules (n) from the same plant, and ovules from the wild type (WT). All ovules were analysed at 12 DAP. Data represent the mean ±SD of three independently extracted RNA samples. (B–E) GUS expression in seeds from the wild type (B) and pCLE19:CLE19G6T:tCLE19 transgenic plants carrying a pMEA:GUS reporter construct, examined at 12 DAP (C–E). Note that aborted seeds in (D) and (E) showed prolonged GUS expression in chalarzal endosperm (marked by asterisks), but not in the normal seed (C) in the same plant. Embryos are traced with dotted lines. c, cotyledon; hy, hypocotyl; h, heart-shaped stage embryo; t, torpedo-stage embryo. Asterisks indicate significant differences from the wild-type (P<0.01 by Student’s t-test). Scale bars in B–E=50 μm.

Mentions: To determine whether expression of the early endosperm-specific genes was altered in pCLE19:CLE19G6T:tCLE19 transgenic plants, expression of MEA, FIS2, and AGL62, which are expressed in the endosperm prior to cellularization (Luo et al., 2000; Kang et al., 2008), was analysed at 10–12 DAP using qRT-PCR. Compared with the wild-type, expression of all these three genes was elevated in those abnormal ovules from pCLE19:CLE19G6T:tCLE19 transgenic plants (Fig. 7A). However, no significant difference in expression levels was seen between wild-type ovules and those normal-looking ovules from the transgenic plants (Fig. 7A).


CLE19 expressed in the embryo regulates both cotyledon establishment and endosperm development in Arabidopsis.

Xu TT, Ren SC, Song XF, Liu CM - J. Exp. Bot. (2015)

Prolonged and elevated expression of early endosperm-specific genes in the endosperm of pCLE19:CLE19G6T:tCLE19 transgenic plants. (A) qRT-PCR analyses showed elevated expression of MEA, FIS2, and AGL62 in those abnormal ovules (a) from a pCLE19:CLE19G6T:tCLE19 transgenic plant (CLE19G6T), as compared with those normal ovules (n) from the same plant, and ovules from the wild type (WT). All ovules were analysed at 12 DAP. Data represent the mean ±SD of three independently extracted RNA samples. (B–E) GUS expression in seeds from the wild type (B) and pCLE19:CLE19G6T:tCLE19 transgenic plants carrying a pMEA:GUS reporter construct, examined at 12 DAP (C–E). Note that aborted seeds in (D) and (E) showed prolonged GUS expression in chalarzal endosperm (marked by asterisks), but not in the normal seed (C) in the same plant. Embryos are traced with dotted lines. c, cotyledon; hy, hypocotyl; h, heart-shaped stage embryo; t, torpedo-stage embryo. Asterisks indicate significant differences from the wild-type (P<0.01 by Student’s t-test). Scale bars in B–E=50 μm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 7: Prolonged and elevated expression of early endosperm-specific genes in the endosperm of pCLE19:CLE19G6T:tCLE19 transgenic plants. (A) qRT-PCR analyses showed elevated expression of MEA, FIS2, and AGL62 in those abnormal ovules (a) from a pCLE19:CLE19G6T:tCLE19 transgenic plant (CLE19G6T), as compared with those normal ovules (n) from the same plant, and ovules from the wild type (WT). All ovules were analysed at 12 DAP. Data represent the mean ±SD of three independently extracted RNA samples. (B–E) GUS expression in seeds from the wild type (B) and pCLE19:CLE19G6T:tCLE19 transgenic plants carrying a pMEA:GUS reporter construct, examined at 12 DAP (C–E). Note that aborted seeds in (D) and (E) showed prolonged GUS expression in chalarzal endosperm (marked by asterisks), but not in the normal seed (C) in the same plant. Embryos are traced with dotted lines. c, cotyledon; hy, hypocotyl; h, heart-shaped stage embryo; t, torpedo-stage embryo. Asterisks indicate significant differences from the wild-type (P<0.01 by Student’s t-test). Scale bars in B–E=50 μm.
Mentions: To determine whether expression of the early endosperm-specific genes was altered in pCLE19:CLE19G6T:tCLE19 transgenic plants, expression of MEA, FIS2, and AGL62, which are expressed in the endosperm prior to cellularization (Luo et al., 2000; Kang et al., 2008), was analysed at 10–12 DAP using qRT-PCR. Compared with the wild-type, expression of all these three genes was elevated in those abnormal ovules from pCLE19:CLE19G6T:tCLE19 transgenic plants (Fig. 7A). However, no significant difference in expression levels was seen between wild-type ovules and those normal-looking ovules from the transgenic plants (Fig. 7A).

Bottom Line: CLE19 is expressed in the epidermal layers of the cotyledon primordia, hypocotyl, and root cap in the embryo.Transgenic plants carrying an antagonistic CLE19 G6T construct expressed under the control of CLE19 regulatory elements exhibited a dominant seed abortion phenotype, with defective cotyledon establishment in embryos and delayed nuclear proliferation and cellularization in endosperms.We therefore propose that CLE19 may act as a mobile peptide co-ordinating embryo and endosperm development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Fragrant Hill, Beijing 100093, China University of Chinese Academy of Sciences, Beijing 100049, China.

No MeSH data available.


Related in: MedlinePlus