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CLE19 expressed in the embryo regulates both cotyledon establishment and endosperm development in Arabidopsis.

Xu TT, Ren SC, Song XF, Liu CM - J. Exp. Bot. (2015)

Bottom Line: CLE19 is expressed in the epidermal layers of the cotyledon primordia, hypocotyl, and root cap in the embryo.Transgenic plants carrying an antagonistic CLE19 G6T construct expressed under the control of CLE19 regulatory elements exhibited a dominant seed abortion phenotype, with defective cotyledon establishment in embryos and delayed nuclear proliferation and cellularization in endosperms.We therefore propose that CLE19 may act as a mobile peptide co-ordinating embryo and endosperm development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Fragrant Hill, Beijing 100093, China University of Chinese Academy of Sciences, Beijing 100049, China.

No MeSH data available.


Related in: MedlinePlus

Delayed embryo sac and endosperm development in pCLE19:CLE19G6T:tCLE19 transgenic plants. (A) Smaller sizes of embryo sacs in pCLE19:CLE19G6T:tCLE19 transgenic plants (CLE19G6T) as compared with those in the wild type (WT). Box plots are used to show total embryo sac areas. The whole area occupied by the embryo and endosperm was measured. Upper and lower bars represent the largest and the smallest sizes of embryo sacs, respectively. Note that smaller embryo sac sizes were observed in transgenic plants at all four stages (globular, triangular, heart-shape, and torpedo) examined (n=30). (B) Lower numbers of endosperm nuclei in pCLE19:CLE19G6T:tCLE19 transgenic plants (CLE19G6T) compared with the wild type (WT) when counted at the triangular stage of embryo development (n=30). Significantly reduced endosperm nuclei numbers (P<0.05 by Welch’s t-test) were observed in transgenic plants (indicated by asterisks).
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Figure 6: Delayed embryo sac and endosperm development in pCLE19:CLE19G6T:tCLE19 transgenic plants. (A) Smaller sizes of embryo sacs in pCLE19:CLE19G6T:tCLE19 transgenic plants (CLE19G6T) as compared with those in the wild type (WT). Box plots are used to show total embryo sac areas. The whole area occupied by the embryo and endosperm was measured. Upper and lower bars represent the largest and the smallest sizes of embryo sacs, respectively. Note that smaller embryo sac sizes were observed in transgenic plants at all four stages (globular, triangular, heart-shape, and torpedo) examined (n=30). (B) Lower numbers of endosperm nuclei in pCLE19:CLE19G6T:tCLE19 transgenic plants (CLE19G6T) compared with the wild type (WT) when counted at the triangular stage of embryo development (n=30). Significantly reduced endosperm nuclei numbers (P<0.05 by Welch’s t-test) were observed in transgenic plants (indicated by asterisks).

Mentions: To examine further the endosperm defect in pCLE19:CLE19G6T:tCLE19 transgenic plants, the size of the embryo sac (the total area occupied by the endosperm and the embryo) was measured in ovules when embryos were at the late globular, triangular, heart-shaped, or torpedo stages. Compared with those in the wild type, embryo sacs in ovules from siliques of pCLE19:CLE19G6T:tCLE19 transgenic plants exhibited much greater variation in size. Many embryo sacs from the transgenic plant were smaller than those from the wild type at the same stage (Fig. 6A). The smallest embryo sac in pCLE19:CLE19G6T:tCLE19 transgenic plants was only half the size of those from the wild type, while the largest embryo sac was not significantly bigger than those from the wild type (Fig. 6A). This result suggests that endosperm development is delayed in pCLE19:CLE19G6T:tCLE19 transgenic plants.


CLE19 expressed in the embryo regulates both cotyledon establishment and endosperm development in Arabidopsis.

Xu TT, Ren SC, Song XF, Liu CM - J. Exp. Bot. (2015)

Delayed embryo sac and endosperm development in pCLE19:CLE19G6T:tCLE19 transgenic plants. (A) Smaller sizes of embryo sacs in pCLE19:CLE19G6T:tCLE19 transgenic plants (CLE19G6T) as compared with those in the wild type (WT). Box plots are used to show total embryo sac areas. The whole area occupied by the embryo and endosperm was measured. Upper and lower bars represent the largest and the smallest sizes of embryo sacs, respectively. Note that smaller embryo sac sizes were observed in transgenic plants at all four stages (globular, triangular, heart-shape, and torpedo) examined (n=30). (B) Lower numbers of endosperm nuclei in pCLE19:CLE19G6T:tCLE19 transgenic plants (CLE19G6T) compared with the wild type (WT) when counted at the triangular stage of embryo development (n=30). Significantly reduced endosperm nuclei numbers (P<0.05 by Welch’s t-test) were observed in transgenic plants (indicated by asterisks).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 6: Delayed embryo sac and endosperm development in pCLE19:CLE19G6T:tCLE19 transgenic plants. (A) Smaller sizes of embryo sacs in pCLE19:CLE19G6T:tCLE19 transgenic plants (CLE19G6T) as compared with those in the wild type (WT). Box plots are used to show total embryo sac areas. The whole area occupied by the embryo and endosperm was measured. Upper and lower bars represent the largest and the smallest sizes of embryo sacs, respectively. Note that smaller embryo sac sizes were observed in transgenic plants at all four stages (globular, triangular, heart-shape, and torpedo) examined (n=30). (B) Lower numbers of endosperm nuclei in pCLE19:CLE19G6T:tCLE19 transgenic plants (CLE19G6T) compared with the wild type (WT) when counted at the triangular stage of embryo development (n=30). Significantly reduced endosperm nuclei numbers (P<0.05 by Welch’s t-test) were observed in transgenic plants (indicated by asterisks).
Mentions: To examine further the endosperm defect in pCLE19:CLE19G6T:tCLE19 transgenic plants, the size of the embryo sac (the total area occupied by the endosperm and the embryo) was measured in ovules when embryos were at the late globular, triangular, heart-shaped, or torpedo stages. Compared with those in the wild type, embryo sacs in ovules from siliques of pCLE19:CLE19G6T:tCLE19 transgenic plants exhibited much greater variation in size. Many embryo sacs from the transgenic plant were smaller than those from the wild type at the same stage (Fig. 6A). The smallest embryo sac in pCLE19:CLE19G6T:tCLE19 transgenic plants was only half the size of those from the wild type, while the largest embryo sac was not significantly bigger than those from the wild type (Fig. 6A). This result suggests that endosperm development is delayed in pCLE19:CLE19G6T:tCLE19 transgenic plants.

Bottom Line: CLE19 is expressed in the epidermal layers of the cotyledon primordia, hypocotyl, and root cap in the embryo.Transgenic plants carrying an antagonistic CLE19 G6T construct expressed under the control of CLE19 regulatory elements exhibited a dominant seed abortion phenotype, with defective cotyledon establishment in embryos and delayed nuclear proliferation and cellularization in endosperms.We therefore propose that CLE19 may act as a mobile peptide co-ordinating embryo and endosperm development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Fragrant Hill, Beijing 100093, China University of Chinese Academy of Sciences, Beijing 100049, China.

No MeSH data available.


Related in: MedlinePlus