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CLE19 expressed in the embryo regulates both cotyledon establishment and endosperm development in Arabidopsis.

Xu TT, Ren SC, Song XF, Liu CM - J. Exp. Bot. (2015)

Bottom Line: CLE19 is expressed in the epidermal layers of the cotyledon primordia, hypocotyl, and root cap in the embryo.Transgenic plants carrying an antagonistic CLE19 G6T construct expressed under the control of CLE19 regulatory elements exhibited a dominant seed abortion phenotype, with defective cotyledon establishment in embryos and delayed nuclear proliferation and cellularization in endosperms.We therefore propose that CLE19 may act as a mobile peptide co-ordinating embryo and endosperm development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Fragrant Hill, Beijing 100093, China University of Chinese Academy of Sciences, Beijing 100049, China.

No MeSH data available.


Related in: MedlinePlus

Embryo culture in vitro did not rescue the defective cotyledon phenotype in embryos excised from pCLE19:CLE19G6T:tCLE19 transgenic plants. Note the abnormal cotyledon establishment in embryos from pCLE19:CLE19G6T:tCLE19 transgenic plants (B, C), as compared with the control embryo from the wild type (A). c, cotyledon; h, hypocotyl. Scale bars=50 μm.
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Figure 4: Embryo culture in vitro did not rescue the defective cotyledon phenotype in embryos excised from pCLE19:CLE19G6T:tCLE19 transgenic plants. Note the abnormal cotyledon establishment in embryos from pCLE19:CLE19G6T:tCLE19 transgenic plants (B, C), as compared with the control embryo from the wild type (A). c, cotyledon; h, hypocotyl. Scale bars=50 μm.

Mentions: The major defect of pCLE19:CLE19G6T:tCLE19 transgenic plants was observed in embryos after the heart-shaped stage. Therefore, in vitro embryo culture was used to examine whether the defective cotyledon development phenotype could be rescued. Early heart-shaped embryos were isolated from the wild-type and transgenic plants and cultured in vitro. After 5 d of cultivation, wild-type embryos had progressed to the cotyledonary stage and had formed two well-established cotyledons (Fig. 4A). However, embryos isolated from pCLE19:CLE19G6T:tCLE19 transgenic plants exhibited a severe defect in cotyledon establishment (Fig. 4B–D), suggesting that the cotyledon arrest was intrinsic to the embryo.


CLE19 expressed in the embryo regulates both cotyledon establishment and endosperm development in Arabidopsis.

Xu TT, Ren SC, Song XF, Liu CM - J. Exp. Bot. (2015)

Embryo culture in vitro did not rescue the defective cotyledon phenotype in embryos excised from pCLE19:CLE19G6T:tCLE19 transgenic plants. Note the abnormal cotyledon establishment in embryos from pCLE19:CLE19G6T:tCLE19 transgenic plants (B, C), as compared with the control embryo from the wild type (A). c, cotyledon; h, hypocotyl. Scale bars=50 μm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526921&req=5

Figure 4: Embryo culture in vitro did not rescue the defective cotyledon phenotype in embryos excised from pCLE19:CLE19G6T:tCLE19 transgenic plants. Note the abnormal cotyledon establishment in embryos from pCLE19:CLE19G6T:tCLE19 transgenic plants (B, C), as compared with the control embryo from the wild type (A). c, cotyledon; h, hypocotyl. Scale bars=50 μm.
Mentions: The major defect of pCLE19:CLE19G6T:tCLE19 transgenic plants was observed in embryos after the heart-shaped stage. Therefore, in vitro embryo culture was used to examine whether the defective cotyledon development phenotype could be rescued. Early heart-shaped embryos were isolated from the wild-type and transgenic plants and cultured in vitro. After 5 d of cultivation, wild-type embryos had progressed to the cotyledonary stage and had formed two well-established cotyledons (Fig. 4A). However, embryos isolated from pCLE19:CLE19G6T:tCLE19 transgenic plants exhibited a severe defect in cotyledon establishment (Fig. 4B–D), suggesting that the cotyledon arrest was intrinsic to the embryo.

Bottom Line: CLE19 is expressed in the epidermal layers of the cotyledon primordia, hypocotyl, and root cap in the embryo.Transgenic plants carrying an antagonistic CLE19 G6T construct expressed under the control of CLE19 regulatory elements exhibited a dominant seed abortion phenotype, with defective cotyledon establishment in embryos and delayed nuclear proliferation and cellularization in endosperms.We therefore propose that CLE19 may act as a mobile peptide co-ordinating embryo and endosperm development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Fragrant Hill, Beijing 100093, China University of Chinese Academy of Sciences, Beijing 100049, China.

No MeSH data available.


Related in: MedlinePlus