Limits...
CLE19 expressed in the embryo regulates both cotyledon establishment and endosperm development in Arabidopsis.

Xu TT, Ren SC, Song XF, Liu CM - J. Exp. Bot. (2015)

Bottom Line: CLE19 is expressed in the epidermal layers of the cotyledon primordia, hypocotyl, and root cap in the embryo.Transgenic plants carrying an antagonistic CLE19 G6T construct expressed under the control of CLE19 regulatory elements exhibited a dominant seed abortion phenotype, with defective cotyledon establishment in embryos and delayed nuclear proliferation and cellularization in endosperms.We therefore propose that CLE19 may act as a mobile peptide co-ordinating embryo and endosperm development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Fragrant Hill, Beijing 100093, China University of Chinese Academy of Sciences, Beijing 100049, China.

No MeSH data available.


Related in: MedlinePlus

Dynamic expression of CLE19 during embryogenesis. As examined under a confocal microscope in embryos excised from pCLE19:SV40-3XGFP:tCLE19 transgenic plants, GFP expression was first observed in protodermal cells (indicated by blue arrowheads) at the lower portion of the 32-cell stage embryo (A), and persisted in their progeny cells in the triangular-stage embryo (B). Additional GFP expression was observed in epidermal cells at the tips and abaxial sides of the cotyledon (indicated by white arrowheads) in heart-shaped (C) and torpedo-stage embryos (D). In walking-stick (E) and cotyledonary-stage embryos (F), strong GFP expression was seen in epidermal cells located at the edges of cotyledons (c, indicated by white arrowheads) and in root caps (indicated by yellow arrowheads), weak GFP expression was observed in the hypocotyl (h). (E) and (F) were prepared by superimposing multiple scanned images. Scale bars=50 μm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4526921&req=5

Figure 3: Dynamic expression of CLE19 during embryogenesis. As examined under a confocal microscope in embryos excised from pCLE19:SV40-3XGFP:tCLE19 transgenic plants, GFP expression was first observed in protodermal cells (indicated by blue arrowheads) at the lower portion of the 32-cell stage embryo (A), and persisted in their progeny cells in the triangular-stage embryo (B). Additional GFP expression was observed in epidermal cells at the tips and abaxial sides of the cotyledon (indicated by white arrowheads) in heart-shaped (C) and torpedo-stage embryos (D). In walking-stick (E) and cotyledonary-stage embryos (F), strong GFP expression was seen in epidermal cells located at the edges of cotyledons (c, indicated by white arrowheads) and in root caps (indicated by yellow arrowheads), weak GFP expression was observed in the hypocotyl (h). (E) and (F) were prepared by superimposing multiple scanned images. Scale bars=50 μm.

Mentions: A pCLE19:SV40-3XGFP:tCLE19 reporter construct with a nuclear-localized triple GFP (SV40-3XGFP) was made and transformed into Arabidopsis (Col-0) to define further the expression pattern of CLE19 in developing embryos. CLSM was performed in embryos dissected from pCLE19:SV40-3XGFP:tCLE19 transgenic plants. GFP expression was first observed in epidermal cells located in the lower portion of the embryo at the late globular stage, and in their progeny cells in triangular-stage embryos (indicated by blue arrowheads; Fig. 3A, B). In heart-shaped and torpedo-stage embryos, additional GFP expression was observed in cotyledon primordia (indicated by white arrowheads; Fig. 3C, D). In walking-stick and cotyledonary-stage embryos, GFP expression was observed in cells located at the edges of the two cotyledons and cells in the root cap (Fig. 3E, F). This expression pattern differs slightly from that observed previously for BnCLE19 from Brassica napus, as no expression was observed in the hypocotyl and root cap regions when the BnCLE19 regulatory elements were examined (Fiers et al., 2004).


CLE19 expressed in the embryo regulates both cotyledon establishment and endosperm development in Arabidopsis.

Xu TT, Ren SC, Song XF, Liu CM - J. Exp. Bot. (2015)

Dynamic expression of CLE19 during embryogenesis. As examined under a confocal microscope in embryos excised from pCLE19:SV40-3XGFP:tCLE19 transgenic plants, GFP expression was first observed in protodermal cells (indicated by blue arrowheads) at the lower portion of the 32-cell stage embryo (A), and persisted in their progeny cells in the triangular-stage embryo (B). Additional GFP expression was observed in epidermal cells at the tips and abaxial sides of the cotyledon (indicated by white arrowheads) in heart-shaped (C) and torpedo-stage embryos (D). In walking-stick (E) and cotyledonary-stage embryos (F), strong GFP expression was seen in epidermal cells located at the edges of cotyledons (c, indicated by white arrowheads) and in root caps (indicated by yellow arrowheads), weak GFP expression was observed in the hypocotyl (h). (E) and (F) were prepared by superimposing multiple scanned images. Scale bars=50 μm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526921&req=5

Figure 3: Dynamic expression of CLE19 during embryogenesis. As examined under a confocal microscope in embryos excised from pCLE19:SV40-3XGFP:tCLE19 transgenic plants, GFP expression was first observed in protodermal cells (indicated by blue arrowheads) at the lower portion of the 32-cell stage embryo (A), and persisted in their progeny cells in the triangular-stage embryo (B). Additional GFP expression was observed in epidermal cells at the tips and abaxial sides of the cotyledon (indicated by white arrowheads) in heart-shaped (C) and torpedo-stage embryos (D). In walking-stick (E) and cotyledonary-stage embryos (F), strong GFP expression was seen in epidermal cells located at the edges of cotyledons (c, indicated by white arrowheads) and in root caps (indicated by yellow arrowheads), weak GFP expression was observed in the hypocotyl (h). (E) and (F) were prepared by superimposing multiple scanned images. Scale bars=50 μm.
Mentions: A pCLE19:SV40-3XGFP:tCLE19 reporter construct with a nuclear-localized triple GFP (SV40-3XGFP) was made and transformed into Arabidopsis (Col-0) to define further the expression pattern of CLE19 in developing embryos. CLSM was performed in embryos dissected from pCLE19:SV40-3XGFP:tCLE19 transgenic plants. GFP expression was first observed in epidermal cells located in the lower portion of the embryo at the late globular stage, and in their progeny cells in triangular-stage embryos (indicated by blue arrowheads; Fig. 3A, B). In heart-shaped and torpedo-stage embryos, additional GFP expression was observed in cotyledon primordia (indicated by white arrowheads; Fig. 3C, D). In walking-stick and cotyledonary-stage embryos, GFP expression was observed in cells located at the edges of the two cotyledons and cells in the root cap (Fig. 3E, F). This expression pattern differs slightly from that observed previously for BnCLE19 from Brassica napus, as no expression was observed in the hypocotyl and root cap regions when the BnCLE19 regulatory elements were examined (Fiers et al., 2004).

Bottom Line: CLE19 is expressed in the epidermal layers of the cotyledon primordia, hypocotyl, and root cap in the embryo.Transgenic plants carrying an antagonistic CLE19 G6T construct expressed under the control of CLE19 regulatory elements exhibited a dominant seed abortion phenotype, with defective cotyledon establishment in embryos and delayed nuclear proliferation and cellularization in endosperms.We therefore propose that CLE19 may act as a mobile peptide co-ordinating embryo and endosperm development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Fragrant Hill, Beijing 100093, China University of Chinese Academy of Sciences, Beijing 100049, China.

No MeSH data available.


Related in: MedlinePlus