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CLE19 expressed in the embryo regulates both cotyledon establishment and endosperm development in Arabidopsis.

Xu TT, Ren SC, Song XF, Liu CM - J. Exp. Bot. (2015)

Bottom Line: CLE19 is expressed in the epidermal layers of the cotyledon primordia, hypocotyl, and root cap in the embryo.Transgenic plants carrying an antagonistic CLE19 G6T construct expressed under the control of CLE19 regulatory elements exhibited a dominant seed abortion phenotype, with defective cotyledon establishment in embryos and delayed nuclear proliferation and cellularization in endosperms.We therefore propose that CLE19 may act as a mobile peptide co-ordinating embryo and endosperm development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Fragrant Hill, Beijing 100093, China University of Chinese Academy of Sciences, Beijing 100049, China.

No MeSH data available.


Related in: MedlinePlus

Embryo-specific expression of CLE19 during seed development. (A–E) GUS expression in seeds excised from pCLE19:GUS:tCLE19 transgenic plants. Note that GUS staining was first detected in late globular-stage embryos (B), and persisted in the triangular (C), heart-shaped (D), and torpedo-stage embryos (E), while no GUS expression was observed in the early globular embryo (A). Scale bars=50 μm. Early embryos (A–C) are indicated by arrowheads; h, heart-shaped embryo; t, torpedo-stage embryo; e, endosperm; s, suspensor. (F) RT–PCR to show CLE19 expression in ovules and embryos, but not in mixed endosperm and testa tissues (En+Ts). EIF4A was used as an internal standard. Control, without cDNA.
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Figure 2: Embryo-specific expression of CLE19 during seed development. (A–E) GUS expression in seeds excised from pCLE19:GUS:tCLE19 transgenic plants. Note that GUS staining was first detected in late globular-stage embryos (B), and persisted in the triangular (C), heart-shaped (D), and torpedo-stage embryos (E), while no GUS expression was observed in the early globular embryo (A). Scale bars=50 μm. Early embryos (A–C) are indicated by arrowheads; h, heart-shaped embryo; t, torpedo-stage embryo; e, endosperm; s, suspensor. (F) RT–PCR to show CLE19 expression in ovules and embryos, but not in mixed endosperm and testa tissues (En+Ts). EIF4A was used as an internal standard. Control, without cDNA.

Mentions: To investigate the expression of CLE19 in Arabidopsis, a pCLE19:GUS:tCLE19 reporter construct was made, with the coding region of pCLE19:CLE19G6T:tCLE19 replaced by the GUS gene, and transformed into Arabidopsis (Col-0). In ovules from the transgenic plants obtained, GUS expression was first observed in embryos at the late globular stage (Fig. 2A, B), and persisted in the triangular (Fig. 2C), heart-shaped (Fig. 2D), and torpedo-stage (Fig. 2E) embryos. No GUS expression was observed in testa or suspensors.


CLE19 expressed in the embryo regulates both cotyledon establishment and endosperm development in Arabidopsis.

Xu TT, Ren SC, Song XF, Liu CM - J. Exp. Bot. (2015)

Embryo-specific expression of CLE19 during seed development. (A–E) GUS expression in seeds excised from pCLE19:GUS:tCLE19 transgenic plants. Note that GUS staining was first detected in late globular-stage embryos (B), and persisted in the triangular (C), heart-shaped (D), and torpedo-stage embryos (E), while no GUS expression was observed in the early globular embryo (A). Scale bars=50 μm. Early embryos (A–C) are indicated by arrowheads; h, heart-shaped embryo; t, torpedo-stage embryo; e, endosperm; s, suspensor. (F) RT–PCR to show CLE19 expression in ovules and embryos, but not in mixed endosperm and testa tissues (En+Ts). EIF4A was used as an internal standard. Control, without cDNA.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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getmorefigures.php?uid=PMC4526921&req=5

Figure 2: Embryo-specific expression of CLE19 during seed development. (A–E) GUS expression in seeds excised from pCLE19:GUS:tCLE19 transgenic plants. Note that GUS staining was first detected in late globular-stage embryos (B), and persisted in the triangular (C), heart-shaped (D), and torpedo-stage embryos (E), while no GUS expression was observed in the early globular embryo (A). Scale bars=50 μm. Early embryos (A–C) are indicated by arrowheads; h, heart-shaped embryo; t, torpedo-stage embryo; e, endosperm; s, suspensor. (F) RT–PCR to show CLE19 expression in ovules and embryos, but not in mixed endosperm and testa tissues (En+Ts). EIF4A was used as an internal standard. Control, without cDNA.
Mentions: To investigate the expression of CLE19 in Arabidopsis, a pCLE19:GUS:tCLE19 reporter construct was made, with the coding region of pCLE19:CLE19G6T:tCLE19 replaced by the GUS gene, and transformed into Arabidopsis (Col-0). In ovules from the transgenic plants obtained, GUS expression was first observed in embryos at the late globular stage (Fig. 2A, B), and persisted in the triangular (Fig. 2C), heart-shaped (Fig. 2D), and torpedo-stage (Fig. 2E) embryos. No GUS expression was observed in testa or suspensors.

Bottom Line: CLE19 is expressed in the epidermal layers of the cotyledon primordia, hypocotyl, and root cap in the embryo.Transgenic plants carrying an antagonistic CLE19 G6T construct expressed under the control of CLE19 regulatory elements exhibited a dominant seed abortion phenotype, with defective cotyledon establishment in embryos and delayed nuclear proliferation and cellularization in endosperms.We therefore propose that CLE19 may act as a mobile peptide co-ordinating embryo and endosperm development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Fragrant Hill, Beijing 100093, China University of Chinese Academy of Sciences, Beijing 100049, China.

No MeSH data available.


Related in: MedlinePlus