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Overexpression of the Arabidopsis thaliana signalling peptide TAXIMIN1 affects lateral organ development.

Colling J, Tohge T, De Clercq R, Brunoud G, Vernoux T, Fernie AR, Makunga NP, Goossens A, Pauwels L - J. Exp. Bot. (2015)

Bottom Line: The first characterization of the secreted cysteine-rich TAXIMIN (TAX) signalling peptides in Arabidopsis is presented here.Nevertheless, TAX1 expression was unchanged in lof1lof2 paraclade junctions and, conversely, LOF gene expression was unchanged in TAX1 overexpressing plants, suggesting TAX1 may act independently.This study identifies TAX1 as the first plant signalling peptide influencing lateral organ separation and implicates the existence of a peptide signal cascade regulating this process in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, Flanders Institute for Biotechnology, (VIB), Technologiepark 927, B-9052 Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, B-9052 Gent, Belgium Institute for Plant Biotechnology, Department of Genetics, Stellenbosch University, Stellenbosch, 7602, South Africa.

No MeSH data available.


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Expression of lateral organ boundary genes does not change in TAX1 overexpressing lines. (A-D) Relative expression of TAX1 and boundary genes in the paraclade junctions of 35S::TAX1 lines (TAX1 OE abbreviated as OE) cultivated in the greenhouse for 5 weeks. Expression values were normalized to those of the wild type (Col-0), set to 1. Values represent the average of three biological replicates ±SE. (E, F) GUS activity in paraclade junctions of 28-day-old mature pLOF2::GUS (E) and pLOF2::GUS x TAX1 OE-3 (F). Arrows indicate the LOF2 expression domain. ax, axillary stem; c, cauline leaf; ps, primary stem. Scale bar = 1mm. (G, H) LOF1 (G) and LOF2 (H) expression in 19-day-old seedlings. (I, J) LOF1 (I) and LOF2 (J) expression in paraclade junctions of 5-week-old tax1tax2 plants. (K, L) TAX1 (K) and TAX2 (L) expression in paraclade junctions of 5-week-old lof1lof2 plants.
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Figure 5: Expression of lateral organ boundary genes does not change in TAX1 overexpressing lines. (A-D) Relative expression of TAX1 and boundary genes in the paraclade junctions of 35S::TAX1 lines (TAX1 OE abbreviated as OE) cultivated in the greenhouse for 5 weeks. Expression values were normalized to those of the wild type (Col-0), set to 1. Values represent the average of three biological replicates ±SE. (E, F) GUS activity in paraclade junctions of 28-day-old mature pLOF2::GUS (E) and pLOF2::GUS x TAX1 OE-3 (F). Arrows indicate the LOF2 expression domain. ax, axillary stem; c, cauline leaf; ps, primary stem. Scale bar = 1mm. (G, H) LOF1 (G) and LOF2 (H) expression in 19-day-old seedlings. (I, J) LOF1 (I) and LOF2 (J) expression in paraclade junctions of 5-week-old tax1tax2 plants. (K, L) TAX1 (K) and TAX2 (L) expression in paraclade junctions of 5-week-old lof1lof2 plants.

Mentions: First, overexpression of TAX1 in the junctions was confirmed by qRT-PCR (Fig. 5A). Line OE-3 showed very high expression of TAX1 at this site, corresponding to the severity of the fusion phenotypes observed in these lines associated with downward bending of the axillary stem (Fig. 3). However, expression of none of the tested boundary genes was significantly altered at this site (Fig. 5B-D). To confirm this finding, a pLOF2::GUS reporter line (Lee et al., 2009) was crossed into the OE-3 background. Both the expression intensity and pattern of pLOF2-driven expression remained unaltered under TAX1 overexpression (Fig. 5E, F), confirming the qRT-PCR results. Likewise, also earlier during development, LOF expression was unchanged in seedlings overexpressing TAX1 (Fig. 5G, H). Finally, when expression of LOF1 and LOF2 was tested in paraclade junctions of the tax1tax2 double mutant, no changes could be observed (Fig. 5I, J). Conversely, to assess whether TAX1 or TAX2 could act downstream of the LOF transcription factors, the paraclade junctions of lof1lof2 mutant plants that show similar fusion defects (Lee et al., 2009) were harvested, but, again, no significant effect on TAX1 and TAX2 expression could be detected (Fig. 5K, L).


Overexpression of the Arabidopsis thaliana signalling peptide TAXIMIN1 affects lateral organ development.

Colling J, Tohge T, De Clercq R, Brunoud G, Vernoux T, Fernie AR, Makunga NP, Goossens A, Pauwels L - J. Exp. Bot. (2015)

Expression of lateral organ boundary genes does not change in TAX1 overexpressing lines. (A-D) Relative expression of TAX1 and boundary genes in the paraclade junctions of 35S::TAX1 lines (TAX1 OE abbreviated as OE) cultivated in the greenhouse for 5 weeks. Expression values were normalized to those of the wild type (Col-0), set to 1. Values represent the average of three biological replicates ±SE. (E, F) GUS activity in paraclade junctions of 28-day-old mature pLOF2::GUS (E) and pLOF2::GUS x TAX1 OE-3 (F). Arrows indicate the LOF2 expression domain. ax, axillary stem; c, cauline leaf; ps, primary stem. Scale bar = 1mm. (G, H) LOF1 (G) and LOF2 (H) expression in 19-day-old seedlings. (I, J) LOF1 (I) and LOF2 (J) expression in paraclade junctions of 5-week-old tax1tax2 plants. (K, L) TAX1 (K) and TAX2 (L) expression in paraclade junctions of 5-week-old lof1lof2 plants.
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Figure 5: Expression of lateral organ boundary genes does not change in TAX1 overexpressing lines. (A-D) Relative expression of TAX1 and boundary genes in the paraclade junctions of 35S::TAX1 lines (TAX1 OE abbreviated as OE) cultivated in the greenhouse for 5 weeks. Expression values were normalized to those of the wild type (Col-0), set to 1. Values represent the average of three biological replicates ±SE. (E, F) GUS activity in paraclade junctions of 28-day-old mature pLOF2::GUS (E) and pLOF2::GUS x TAX1 OE-3 (F). Arrows indicate the LOF2 expression domain. ax, axillary stem; c, cauline leaf; ps, primary stem. Scale bar = 1mm. (G, H) LOF1 (G) and LOF2 (H) expression in 19-day-old seedlings. (I, J) LOF1 (I) and LOF2 (J) expression in paraclade junctions of 5-week-old tax1tax2 plants. (K, L) TAX1 (K) and TAX2 (L) expression in paraclade junctions of 5-week-old lof1lof2 plants.
Mentions: First, overexpression of TAX1 in the junctions was confirmed by qRT-PCR (Fig. 5A). Line OE-3 showed very high expression of TAX1 at this site, corresponding to the severity of the fusion phenotypes observed in these lines associated with downward bending of the axillary stem (Fig. 3). However, expression of none of the tested boundary genes was significantly altered at this site (Fig. 5B-D). To confirm this finding, a pLOF2::GUS reporter line (Lee et al., 2009) was crossed into the OE-3 background. Both the expression intensity and pattern of pLOF2-driven expression remained unaltered under TAX1 overexpression (Fig. 5E, F), confirming the qRT-PCR results. Likewise, also earlier during development, LOF expression was unchanged in seedlings overexpressing TAX1 (Fig. 5G, H). Finally, when expression of LOF1 and LOF2 was tested in paraclade junctions of the tax1tax2 double mutant, no changes could be observed (Fig. 5I, J). Conversely, to assess whether TAX1 or TAX2 could act downstream of the LOF transcription factors, the paraclade junctions of lof1lof2 mutant plants that show similar fusion defects (Lee et al., 2009) were harvested, but, again, no significant effect on TAX1 and TAX2 expression could be detected (Fig. 5K, L).

Bottom Line: The first characterization of the secreted cysteine-rich TAXIMIN (TAX) signalling peptides in Arabidopsis is presented here.Nevertheless, TAX1 expression was unchanged in lof1lof2 paraclade junctions and, conversely, LOF gene expression was unchanged in TAX1 overexpressing plants, suggesting TAX1 may act independently.This study identifies TAX1 as the first plant signalling peptide influencing lateral organ separation and implicates the existence of a peptide signal cascade regulating this process in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, Flanders Institute for Biotechnology, (VIB), Technologiepark 927, B-9052 Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, B-9052 Gent, Belgium Institute for Plant Biotechnology, Department of Genetics, Stellenbosch University, Stellenbosch, 7602, South Africa.

No MeSH data available.


Related in: MedlinePlus