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Overexpression of the Arabidopsis thaliana signalling peptide TAXIMIN1 affects lateral organ development.

Colling J, Tohge T, De Clercq R, Brunoud G, Vernoux T, Fernie AR, Makunga NP, Goossens A, Pauwels L - J. Exp. Bot. (2015)

Bottom Line: The first characterization of the secreted cysteine-rich TAXIMIN (TAX) signalling peptides in Arabidopsis is presented here.Nevertheless, TAX1 expression was unchanged in lof1lof2 paraclade junctions and, conversely, LOF gene expression was unchanged in TAX1 overexpressing plants, suggesting TAX1 may act independently.This study identifies TAX1 as the first plant signalling peptide influencing lateral organ separation and implicates the existence of a peptide signal cascade regulating this process in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, Flanders Institute for Biotechnology, (VIB), Technologiepark 927, B-9052 Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, B-9052 Gent, Belgium Institute for Plant Biotechnology, Department of Genetics, Stellenbosch University, Stellenbosch, 7602, South Africa.

No MeSH data available.


Related in: MedlinePlus

TAX1 and TAX2 have distinct promoter activities. Plants expressing a nuclear localized GUS-GFP fusion under control of the TAX1 (A-I) and TAX2 (J-L) promoter were used. (A, B) GUS activity in 10-day-old seedlings. (C-E) GFP signal at the shoot apical meristem visualized by confocal microscopy. (C) Stacked image. Primordia (P) are numbered from youngest to oldest. (D) Longitudinal optical section at P2. (E) Transverse optical section of the centre of the meristem (M). L1 and L2 layers are indicated. (F-H) GUS activity in the flower (F, G) and the paraclade junction (H) of 28-day-old mature plants. (I) GUS activity at the shoot apical meristem is also visible in a second TAX1 reporter line that had lower GUS expression levels. (J-L) pTAX2::GUS activity in 10-day-old seedlings (J), the flower (K), and the paraclade junction (L) of 28-day-old mature plants. Abbreviations: ax, axillary stem; c, cauline leaf; ps, primary stem. All scale bars are 1mm.
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Figure 4: TAX1 and TAX2 have distinct promoter activities. Plants expressing a nuclear localized GUS-GFP fusion under control of the TAX1 (A-I) and TAX2 (J-L) promoter were used. (A, B) GUS activity in 10-day-old seedlings. (C-E) GFP signal at the shoot apical meristem visualized by confocal microscopy. (C) Stacked image. Primordia (P) are numbered from youngest to oldest. (D) Longitudinal optical section at P2. (E) Transverse optical section of the centre of the meristem (M). L1 and L2 layers are indicated. (F-H) GUS activity in the flower (F, G) and the paraclade junction (H) of 28-day-old mature plants. (I) GUS activity at the shoot apical meristem is also visible in a second TAX1 reporter line that had lower GUS expression levels. (J-L) pTAX2::GUS activity in 10-day-old seedlings (J), the flower (K), and the paraclade junction (L) of 28-day-old mature plants. Abbreviations: ax, axillary stem; c, cauline leaf; ps, primary stem. All scale bars are 1mm.

Mentions: In 10-day-old in vitro-germinated pTAX1::GUS:GFP seedlings, the GUS expression was detected mainly in the SAM region (Fig. 4A, B, I). Using GFP, TAX1 promoter activity was detected in the entire inflorescence meristem of 5-week-old plants, but was stronger in the organ primordia (Fig. 4C-E). TAX1 expression was mostly specific to the L1 layer in the centre of the meristem but was also detected in the L2 layer in organ primordia (Fig. 4C-E).


Overexpression of the Arabidopsis thaliana signalling peptide TAXIMIN1 affects lateral organ development.

Colling J, Tohge T, De Clercq R, Brunoud G, Vernoux T, Fernie AR, Makunga NP, Goossens A, Pauwels L - J. Exp. Bot. (2015)

TAX1 and TAX2 have distinct promoter activities. Plants expressing a nuclear localized GUS-GFP fusion under control of the TAX1 (A-I) and TAX2 (J-L) promoter were used. (A, B) GUS activity in 10-day-old seedlings. (C-E) GFP signal at the shoot apical meristem visualized by confocal microscopy. (C) Stacked image. Primordia (P) are numbered from youngest to oldest. (D) Longitudinal optical section at P2. (E) Transverse optical section of the centre of the meristem (M). L1 and L2 layers are indicated. (F-H) GUS activity in the flower (F, G) and the paraclade junction (H) of 28-day-old mature plants. (I) GUS activity at the shoot apical meristem is also visible in a second TAX1 reporter line that had lower GUS expression levels. (J-L) pTAX2::GUS activity in 10-day-old seedlings (J), the flower (K), and the paraclade junction (L) of 28-day-old mature plants. Abbreviations: ax, axillary stem; c, cauline leaf; ps, primary stem. All scale bars are 1mm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526920&req=5

Figure 4: TAX1 and TAX2 have distinct promoter activities. Plants expressing a nuclear localized GUS-GFP fusion under control of the TAX1 (A-I) and TAX2 (J-L) promoter were used. (A, B) GUS activity in 10-day-old seedlings. (C-E) GFP signal at the shoot apical meristem visualized by confocal microscopy. (C) Stacked image. Primordia (P) are numbered from youngest to oldest. (D) Longitudinal optical section at P2. (E) Transverse optical section of the centre of the meristem (M). L1 and L2 layers are indicated. (F-H) GUS activity in the flower (F, G) and the paraclade junction (H) of 28-day-old mature plants. (I) GUS activity at the shoot apical meristem is also visible in a second TAX1 reporter line that had lower GUS expression levels. (J-L) pTAX2::GUS activity in 10-day-old seedlings (J), the flower (K), and the paraclade junction (L) of 28-day-old mature plants. Abbreviations: ax, axillary stem; c, cauline leaf; ps, primary stem. All scale bars are 1mm.
Mentions: In 10-day-old in vitro-germinated pTAX1::GUS:GFP seedlings, the GUS expression was detected mainly in the SAM region (Fig. 4A, B, I). Using GFP, TAX1 promoter activity was detected in the entire inflorescence meristem of 5-week-old plants, but was stronger in the organ primordia (Fig. 4C-E). TAX1 expression was mostly specific to the L1 layer in the centre of the meristem but was also detected in the L2 layer in organ primordia (Fig. 4C-E).

Bottom Line: The first characterization of the secreted cysteine-rich TAXIMIN (TAX) signalling peptides in Arabidopsis is presented here.Nevertheless, TAX1 expression was unchanged in lof1lof2 paraclade junctions and, conversely, LOF gene expression was unchanged in TAX1 overexpressing plants, suggesting TAX1 may act independently.This study identifies TAX1 as the first plant signalling peptide influencing lateral organ separation and implicates the existence of a peptide signal cascade regulating this process in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, Flanders Institute for Biotechnology, (VIB), Technologiepark 927, B-9052 Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, B-9052 Gent, Belgium Institute for Plant Biotechnology, Department of Genetics, Stellenbosch University, Stellenbosch, 7602, South Africa.

No MeSH data available.


Related in: MedlinePlus