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Overexpression of the Arabidopsis thaliana signalling peptide TAXIMIN1 affects lateral organ development.

Colling J, Tohge T, De Clercq R, Brunoud G, Vernoux T, Fernie AR, Makunga NP, Goossens A, Pauwels L - J. Exp. Bot. (2015)

Bottom Line: The first characterization of the secreted cysteine-rich TAXIMIN (TAX) signalling peptides in Arabidopsis is presented here.Nevertheless, TAX1 expression was unchanged in lof1lof2 paraclade junctions and, conversely, LOF gene expression was unchanged in TAX1 overexpressing plants, suggesting TAX1 may act independently.This study identifies TAX1 as the first plant signalling peptide influencing lateral organ separation and implicates the existence of a peptide signal cascade regulating this process in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, Flanders Institute for Biotechnology, (VIB), Technologiepark 927, B-9052 Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, B-9052 Gent, Belgium Institute for Plant Biotechnology, Department of Genetics, Stellenbosch University, Stellenbosch, 7602, South Africa.

No MeSH data available.


Related in: MedlinePlus

Constitutive TAX1 overexpression results in lateral organ fusion with reduced penetrance. (A) Schematic overview of the location and frequency of phenotypes observed in line OE-3. (B) Undifferentiated outgrowths (indicated by arrow) at the inflorescence. (C) Side view of the protrusion (indicated by the arrow) of the primary stem at the first node, accompanied with fusion of the broader cauline leaf base to the axillary stem which deflects downwards. (D) Top view of node 2 with a broader cauline leaf base. (E) Fusion of the cauline leaf to both the axillary stem and the primary stem at node 3. (F) Fusion of cauline leaves to the axillary stem that originates from the rosette and to the accessory shoot when there is no third node. Abbreviations: ax, axillary stem; ac, accessory shoot; ps, primary stem.
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Figure 3: Constitutive TAX1 overexpression results in lateral organ fusion with reduced penetrance. (A) Schematic overview of the location and frequency of phenotypes observed in line OE-3. (B) Undifferentiated outgrowths (indicated by arrow) at the inflorescence. (C) Side view of the protrusion (indicated by the arrow) of the primary stem at the first node, accompanied with fusion of the broader cauline leaf base to the axillary stem which deflects downwards. (D) Top view of node 2 with a broader cauline leaf base. (E) Fusion of the cauline leaf to both the axillary stem and the primary stem at node 3. (F) Fusion of cauline leaves to the axillary stem that originates from the rosette and to the accessory shoot when there is no third node. Abbreviations: ax, axillary stem; ac, accessory shoot; ps, primary stem.

Mentions: The highest expressing TAX1 overexpression lines OE-2 and OE-3 showed reduced growth of the seedlings on basal MS plates (Fig. 2A, Supplementary Fig. S2A at JXB online). After transfer to the greenhouse, these lines were delayed in development (Supplementary Fig. S2B, C at JXB online). Importantly though, TAX1 overexpressing lines OE-2 and OE-3 showed developmental defects at paraclade junctions and altered fruit morphology (Figs 2 and 3).


Overexpression of the Arabidopsis thaliana signalling peptide TAXIMIN1 affects lateral organ development.

Colling J, Tohge T, De Clercq R, Brunoud G, Vernoux T, Fernie AR, Makunga NP, Goossens A, Pauwels L - J. Exp. Bot. (2015)

Constitutive TAX1 overexpression results in lateral organ fusion with reduced penetrance. (A) Schematic overview of the location and frequency of phenotypes observed in line OE-3. (B) Undifferentiated outgrowths (indicated by arrow) at the inflorescence. (C) Side view of the protrusion (indicated by the arrow) of the primary stem at the first node, accompanied with fusion of the broader cauline leaf base to the axillary stem which deflects downwards. (D) Top view of node 2 with a broader cauline leaf base. (E) Fusion of the cauline leaf to both the axillary stem and the primary stem at node 3. (F) Fusion of cauline leaves to the axillary stem that originates from the rosette and to the accessory shoot when there is no third node. Abbreviations: ax, axillary stem; ac, accessory shoot; ps, primary stem.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526920&req=5

Figure 3: Constitutive TAX1 overexpression results in lateral organ fusion with reduced penetrance. (A) Schematic overview of the location and frequency of phenotypes observed in line OE-3. (B) Undifferentiated outgrowths (indicated by arrow) at the inflorescence. (C) Side view of the protrusion (indicated by the arrow) of the primary stem at the first node, accompanied with fusion of the broader cauline leaf base to the axillary stem which deflects downwards. (D) Top view of node 2 with a broader cauline leaf base. (E) Fusion of the cauline leaf to both the axillary stem and the primary stem at node 3. (F) Fusion of cauline leaves to the axillary stem that originates from the rosette and to the accessory shoot when there is no third node. Abbreviations: ax, axillary stem; ac, accessory shoot; ps, primary stem.
Mentions: The highest expressing TAX1 overexpression lines OE-2 and OE-3 showed reduced growth of the seedlings on basal MS plates (Fig. 2A, Supplementary Fig. S2A at JXB online). After transfer to the greenhouse, these lines were delayed in development (Supplementary Fig. S2B, C at JXB online). Importantly though, TAX1 overexpressing lines OE-2 and OE-3 showed developmental defects at paraclade junctions and altered fruit morphology (Figs 2 and 3).

Bottom Line: The first characterization of the secreted cysteine-rich TAXIMIN (TAX) signalling peptides in Arabidopsis is presented here.Nevertheless, TAX1 expression was unchanged in lof1lof2 paraclade junctions and, conversely, LOF gene expression was unchanged in TAX1 overexpressing plants, suggesting TAX1 may act independently.This study identifies TAX1 as the first plant signalling peptide influencing lateral organ separation and implicates the existence of a peptide signal cascade regulating this process in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, Flanders Institute for Biotechnology, (VIB), Technologiepark 927, B-9052 Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, B-9052 Gent, Belgium Institute for Plant Biotechnology, Department of Genetics, Stellenbosch University, Stellenbosch, 7602, South Africa.

No MeSH data available.


Related in: MedlinePlus