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Overexpression of the Arabidopsis thaliana signalling peptide TAXIMIN1 affects lateral organ development.

Colling J, Tohge T, De Clercq R, Brunoud G, Vernoux T, Fernie AR, Makunga NP, Goossens A, Pauwels L - J. Exp. Bot. (2015)

Bottom Line: The first characterization of the secreted cysteine-rich TAXIMIN (TAX) signalling peptides in Arabidopsis is presented here.Nevertheless, TAX1 expression was unchanged in lof1lof2 paraclade junctions and, conversely, LOF gene expression was unchanged in TAX1 overexpressing plants, suggesting TAX1 may act independently.This study identifies TAX1 as the first plant signalling peptide influencing lateral organ separation and implicates the existence of a peptide signal cascade regulating this process in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, Flanders Institute for Biotechnology, (VIB), Technologiepark 927, B-9052 Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, B-9052 Gent, Belgium Institute for Plant Biotechnology, Department of Genetics, Stellenbosch University, Stellenbosch, 7602, South Africa.

No MeSH data available.


Related in: MedlinePlus

TAX1 overexpression in Col-0 and Ler backgrounds results in an alteration of fruit morphology. (A) Relative expression of TAX1 in 19-day-old seedlings compared to the Col-0 wild type for three independent 35S::TAX1 lines. Expression values were normalized to those of the wild type (Col-0), set to 1. Values represent the average of three biological replicates ±SE. (B) Medial and lateral view of mature siliques of TAX1 overexpression (OE) lines. (C) Lateral view with seeds removed. Arrow indicates protrusion of the replum at the tip of the silique in TAX1 overexpressing lines. (D) Lateral silique view with one valve removed. (E) Scanning electron microscopy images of early stage gynoecia of TAX1 OE lines. Arrow indicates protrusion. The scale bar is 400 µm. (F) Relative expression of TAX1 in 19-day-old seedlings compared to the Ler wild type for four independent 35S::TAX1 lines. Expression values were normalized to those of the wild type (Ler). Values represent the average of four biological replicates ±SE. (G) Medial and lateral view of mature siliques of TAX1 OE lines in the same order as in (F). Scale bars in B-D and G are 5mm.
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Figure 2: TAX1 overexpression in Col-0 and Ler backgrounds results in an alteration of fruit morphology. (A) Relative expression of TAX1 in 19-day-old seedlings compared to the Col-0 wild type for three independent 35S::TAX1 lines. Expression values were normalized to those of the wild type (Col-0), set to 1. Values represent the average of three biological replicates ±SE. (B) Medial and lateral view of mature siliques of TAX1 overexpression (OE) lines. (C) Lateral view with seeds removed. Arrow indicates protrusion of the replum at the tip of the silique in TAX1 overexpressing lines. (D) Lateral silique view with one valve removed. (E) Scanning electron microscopy images of early stage gynoecia of TAX1 OE lines. Arrow indicates protrusion. The scale bar is 400 µm. (F) Relative expression of TAX1 in 19-day-old seedlings compared to the Ler wild type for four independent 35S::TAX1 lines. Expression values were normalized to those of the wild type (Ler). Values represent the average of four biological replicates ±SE. (G) Medial and lateral view of mature siliques of TAX1 OE lines in the same order as in (F). Scale bars in B-D and G are 5mm.

Mentions: The highest expressing TAX1 overexpression lines OE-2 and OE-3 showed reduced growth of the seedlings on basal MS plates (Fig. 2A, Supplementary Fig. S2A at JXB online). After transfer to the greenhouse, these lines were delayed in development (Supplementary Fig. S2B, C at JXB online). Importantly though, TAX1 overexpressing lines OE-2 and OE-3 showed developmental defects at paraclade junctions and altered fruit morphology (Figs 2 and 3).


Overexpression of the Arabidopsis thaliana signalling peptide TAXIMIN1 affects lateral organ development.

Colling J, Tohge T, De Clercq R, Brunoud G, Vernoux T, Fernie AR, Makunga NP, Goossens A, Pauwels L - J. Exp. Bot. (2015)

TAX1 overexpression in Col-0 and Ler backgrounds results in an alteration of fruit morphology. (A) Relative expression of TAX1 in 19-day-old seedlings compared to the Col-0 wild type for three independent 35S::TAX1 lines. Expression values were normalized to those of the wild type (Col-0), set to 1. Values represent the average of three biological replicates ±SE. (B) Medial and lateral view of mature siliques of TAX1 overexpression (OE) lines. (C) Lateral view with seeds removed. Arrow indicates protrusion of the replum at the tip of the silique in TAX1 overexpressing lines. (D) Lateral silique view with one valve removed. (E) Scanning electron microscopy images of early stage gynoecia of TAX1 OE lines. Arrow indicates protrusion. The scale bar is 400 µm. (F) Relative expression of TAX1 in 19-day-old seedlings compared to the Ler wild type for four independent 35S::TAX1 lines. Expression values were normalized to those of the wild type (Ler). Values represent the average of four biological replicates ±SE. (G) Medial and lateral view of mature siliques of TAX1 OE lines in the same order as in (F). Scale bars in B-D and G are 5mm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 2: TAX1 overexpression in Col-0 and Ler backgrounds results in an alteration of fruit morphology. (A) Relative expression of TAX1 in 19-day-old seedlings compared to the Col-0 wild type for three independent 35S::TAX1 lines. Expression values were normalized to those of the wild type (Col-0), set to 1. Values represent the average of three biological replicates ±SE. (B) Medial and lateral view of mature siliques of TAX1 overexpression (OE) lines. (C) Lateral view with seeds removed. Arrow indicates protrusion of the replum at the tip of the silique in TAX1 overexpressing lines. (D) Lateral silique view with one valve removed. (E) Scanning electron microscopy images of early stage gynoecia of TAX1 OE lines. Arrow indicates protrusion. The scale bar is 400 µm. (F) Relative expression of TAX1 in 19-day-old seedlings compared to the Ler wild type for four independent 35S::TAX1 lines. Expression values were normalized to those of the wild type (Ler). Values represent the average of four biological replicates ±SE. (G) Medial and lateral view of mature siliques of TAX1 OE lines in the same order as in (F). Scale bars in B-D and G are 5mm.
Mentions: The highest expressing TAX1 overexpression lines OE-2 and OE-3 showed reduced growth of the seedlings on basal MS plates (Fig. 2A, Supplementary Fig. S2A at JXB online). After transfer to the greenhouse, these lines were delayed in development (Supplementary Fig. S2B, C at JXB online). Importantly though, TAX1 overexpressing lines OE-2 and OE-3 showed developmental defects at paraclade junctions and altered fruit morphology (Figs 2 and 3).

Bottom Line: The first characterization of the secreted cysteine-rich TAXIMIN (TAX) signalling peptides in Arabidopsis is presented here.Nevertheless, TAX1 expression was unchanged in lof1lof2 paraclade junctions and, conversely, LOF gene expression was unchanged in TAX1 overexpressing plants, suggesting TAX1 may act independently.This study identifies TAX1 as the first plant signalling peptide influencing lateral organ separation and implicates the existence of a peptide signal cascade regulating this process in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, Flanders Institute for Biotechnology, (VIB), Technologiepark 927, B-9052 Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, B-9052 Gent, Belgium Institute for Plant Biotechnology, Department of Genetics, Stellenbosch University, Stellenbosch, 7602, South Africa.

No MeSH data available.


Related in: MedlinePlus