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Overexpression of the Arabidopsis thaliana signalling peptide TAXIMIN1 affects lateral organ development.

Colling J, Tohge T, De Clercq R, Brunoud G, Vernoux T, Fernie AR, Makunga NP, Goossens A, Pauwels L - J. Exp. Bot. (2015)

Bottom Line: The first characterization of the secreted cysteine-rich TAXIMIN (TAX) signalling peptides in Arabidopsis is presented here.Nevertheless, TAX1 expression was unchanged in lof1lof2 paraclade junctions and, conversely, LOF gene expression was unchanged in TAX1 overexpressing plants, suggesting TAX1 may act independently.This study identifies TAX1 as the first plant signalling peptide influencing lateral organ separation and implicates the existence of a peptide signal cascade regulating this process in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, Flanders Institute for Biotechnology, (VIB), Technologiepark 927, B-9052 Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, B-9052 Gent, Belgium Institute for Plant Biotechnology, Department of Genetics, Stellenbosch University, Stellenbosch, 7602, South Africa.

No MeSH data available.


Related in: MedlinePlus

The TAXIMIN peptides in Arabidopsis. (A) Sequence alignment of TbTAX and Arabidopsis orthologues TAX1 and TAX2. The in silico-predicted TbTAX signal peptide (Onrubia et al., 2014), located at the N-terminus, and hydrophobic amino acids are underlined and marked by circles, respectively. (B) Schematic diagram of the gene structure of TAX1 and TAX2 displaying the site of T-DNA insertion in the respective tax mutant lines. Black bars, grey bars, and black lines represent exons, UTR regions, and introns, respectively. (C, D) Subcellular localization of the TAX1 peptide with (C) and without (D) the N-terminal signal fused to Venus expressed in 5-day-old Arabidopsis root cells and visualized with a confocal microscope. Propidium iodide (PI) staining was used as a localization control. Scale bars are 20 µm.
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Figure 1: The TAXIMIN peptides in Arabidopsis. (A) Sequence alignment of TbTAX and Arabidopsis orthologues TAX1 and TAX2. The in silico-predicted TbTAX signal peptide (Onrubia et al., 2014), located at the N-terminus, and hydrophobic amino acids are underlined and marked by circles, respectively. (B) Schematic diagram of the gene structure of TAX1 and TAX2 displaying the site of T-DNA insertion in the respective tax mutant lines. Black bars, grey bars, and black lines represent exons, UTR regions, and introns, respectively. (C, D) Subcellular localization of the TAX1 peptide with (C) and without (D) the N-terminal signal fused to Venus expressed in 5-day-old Arabidopsis root cells and visualized with a confocal microscope. Propidium iodide (PI) staining was used as a localization control. Scale bars are 20 µm.

Mentions: Imaging of living SAMs of 5-week-old plants was performed using a LSM700 laser-scanning confocal microscope (Zeiss, Jena, Germany). TAX-Venus fusions were imaged in 5- or 10-day-old seedlings with an Olympus FV10 ASW confocal microscope. Images in Fig. 1 and Supplementary Fig. S1 at JXB online are from independent transformed lines. Before imaging, seedlings were briefly incubated in propidium iodide (3mg/L, Sigma) and subsequently washed and mounted in water. For scanning electron microscopy of gynoecia, flowers at stage 13 were collected from wild-type and TAX1 overexpressing plants cultivated in the greenhouse. Sepals, petals, and stamen were removed to reveal the carpel, which was directly mounted on the steel stubs. Images were collected using a Hitachi TM-1000 table-top scanning electron microscope (Hitachi High-Technologies Corporation).


Overexpression of the Arabidopsis thaliana signalling peptide TAXIMIN1 affects lateral organ development.

Colling J, Tohge T, De Clercq R, Brunoud G, Vernoux T, Fernie AR, Makunga NP, Goossens A, Pauwels L - J. Exp. Bot. (2015)

The TAXIMIN peptides in Arabidopsis. (A) Sequence alignment of TbTAX and Arabidopsis orthologues TAX1 and TAX2. The in silico-predicted TbTAX signal peptide (Onrubia et al., 2014), located at the N-terminus, and hydrophobic amino acids are underlined and marked by circles, respectively. (B) Schematic diagram of the gene structure of TAX1 and TAX2 displaying the site of T-DNA insertion in the respective tax mutant lines. Black bars, grey bars, and black lines represent exons, UTR regions, and introns, respectively. (C, D) Subcellular localization of the TAX1 peptide with (C) and without (D) the N-terminal signal fused to Venus expressed in 5-day-old Arabidopsis root cells and visualized with a confocal microscope. Propidium iodide (PI) staining was used as a localization control. Scale bars are 20 µm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526920&req=5

Figure 1: The TAXIMIN peptides in Arabidopsis. (A) Sequence alignment of TbTAX and Arabidopsis orthologues TAX1 and TAX2. The in silico-predicted TbTAX signal peptide (Onrubia et al., 2014), located at the N-terminus, and hydrophobic amino acids are underlined and marked by circles, respectively. (B) Schematic diagram of the gene structure of TAX1 and TAX2 displaying the site of T-DNA insertion in the respective tax mutant lines. Black bars, grey bars, and black lines represent exons, UTR regions, and introns, respectively. (C, D) Subcellular localization of the TAX1 peptide with (C) and without (D) the N-terminal signal fused to Venus expressed in 5-day-old Arabidopsis root cells and visualized with a confocal microscope. Propidium iodide (PI) staining was used as a localization control. Scale bars are 20 µm.
Mentions: Imaging of living SAMs of 5-week-old plants was performed using a LSM700 laser-scanning confocal microscope (Zeiss, Jena, Germany). TAX-Venus fusions were imaged in 5- or 10-day-old seedlings with an Olympus FV10 ASW confocal microscope. Images in Fig. 1 and Supplementary Fig. S1 at JXB online are from independent transformed lines. Before imaging, seedlings were briefly incubated in propidium iodide (3mg/L, Sigma) and subsequently washed and mounted in water. For scanning electron microscopy of gynoecia, flowers at stage 13 were collected from wild-type and TAX1 overexpressing plants cultivated in the greenhouse. Sepals, petals, and stamen were removed to reveal the carpel, which was directly mounted on the steel stubs. Images were collected using a Hitachi TM-1000 table-top scanning electron microscope (Hitachi High-Technologies Corporation).

Bottom Line: The first characterization of the secreted cysteine-rich TAXIMIN (TAX) signalling peptides in Arabidopsis is presented here.Nevertheless, TAX1 expression was unchanged in lof1lof2 paraclade junctions and, conversely, LOF gene expression was unchanged in TAX1 overexpressing plants, suggesting TAX1 may act independently.This study identifies TAX1 as the first plant signalling peptide influencing lateral organ separation and implicates the existence of a peptide signal cascade regulating this process in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Systems Biology, Flanders Institute for Biotechnology, (VIB), Technologiepark 927, B-9052 Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, B-9052 Gent, Belgium Institute for Plant Biotechnology, Department of Genetics, Stellenbosch University, Stellenbosch, 7602, South Africa.

No MeSH data available.


Related in: MedlinePlus