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The IDA/IDA-LIKE and PIP/PIP-LIKE gene families in Arabidopsis: phylogenetic relationship, expression patterns, and transcriptional effect of the PIPL3 peptide.

Vie AK, Najafi J, Liu B, Winge P, Butenko MA, Hornslien KS, Kumpf R, Aalen RB, Bones AM, Brembu T - J. Exp. Bot. (2015)

Bottom Line: Here we present three novel members of the IDL subfamily and show that two of them are strongly and rapidly induced by different biotic and abiotic stresses.Expression patterns of the IDA/IDL and PIP/PIPL genes were analysed using in silico data, qRT-PCR and GUS promoter lines.Transcriptomic responses to PIPL3 peptide treatment suggested a role in regulation of biotic stress responses and cell wall modification.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

No MeSH data available.


Related in: MedlinePlus

Transcriptional responses to PIPL3 peptide treatment. GO enrichment analysis of significantly regulated (P<0.05) genes in 2-week-old seedlings 3h after treatment with 100nM PIPL3 peptide. (A) Up-regulated genes; (B) Down-regulated genes. Control seedlings were treated with 100nM mock peptide. The dataset was filtered for expression ratio ratios (>log2 1.0, <log2 −0.7). The 10 most significantly enriched terms are listed from top to bottom. The bars show the frequency ratios of each GO term in the PIPL3-responsive gene set versus the genome.
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Figure 9: Transcriptional responses to PIPL3 peptide treatment. GO enrichment analysis of significantly regulated (P<0.05) genes in 2-week-old seedlings 3h after treatment with 100nM PIPL3 peptide. (A) Up-regulated genes; (B) Down-regulated genes. Control seedlings were treated with 100nM mock peptide. The dataset was filtered for expression ratio ratios (>log2 1.0, <log2 −0.7). The 10 most significantly enriched terms are listed from top to bottom. The bars show the frequency ratios of each GO term in the PIPL3-responsive gene set versus the genome.

Mentions: Treatment with 100nM PIPL3 peptide (containing a hydroxyproline in position 15) for 3h led to a widespread response compared with mock peptide-treated seedlings: 1599 genes were significantly (P<0.05) induced, whereas 1133 genes were significantly repressed. Genes showing low expression ratios (>log2 1.0, <log2 −0.7) were removed, and the filtered dataset (291 induced and 129 repressed genes, respectively, Supplementary Dataset S3) was subjected to a Gene Ontology (GO) enrichment analysis (Ashburner et al., 2000). The ten GO biological process categories most overrepresented in the up-regulated gene set indicated that the peptide treatment induced processes related to biotic stress and responses to chemical substances (possibly derived from other organisms) (Fig. 9A). The GO categories most enriched among the down-regulated genes were related to cell wall modification and loosening (Fig. 9B).


The IDA/IDA-LIKE and PIP/PIP-LIKE gene families in Arabidopsis: phylogenetic relationship, expression patterns, and transcriptional effect of the PIPL3 peptide.

Vie AK, Najafi J, Liu B, Winge P, Butenko MA, Hornslien KS, Kumpf R, Aalen RB, Bones AM, Brembu T - J. Exp. Bot. (2015)

Transcriptional responses to PIPL3 peptide treatment. GO enrichment analysis of significantly regulated (P<0.05) genes in 2-week-old seedlings 3h after treatment with 100nM PIPL3 peptide. (A) Up-regulated genes; (B) Down-regulated genes. Control seedlings were treated with 100nM mock peptide. The dataset was filtered for expression ratio ratios (>log2 1.0, <log2 −0.7). The 10 most significantly enriched terms are listed from top to bottom. The bars show the frequency ratios of each GO term in the PIPL3-responsive gene set versus the genome.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4526919&req=5

Figure 9: Transcriptional responses to PIPL3 peptide treatment. GO enrichment analysis of significantly regulated (P<0.05) genes in 2-week-old seedlings 3h after treatment with 100nM PIPL3 peptide. (A) Up-regulated genes; (B) Down-regulated genes. Control seedlings were treated with 100nM mock peptide. The dataset was filtered for expression ratio ratios (>log2 1.0, <log2 −0.7). The 10 most significantly enriched terms are listed from top to bottom. The bars show the frequency ratios of each GO term in the PIPL3-responsive gene set versus the genome.
Mentions: Treatment with 100nM PIPL3 peptide (containing a hydroxyproline in position 15) for 3h led to a widespread response compared with mock peptide-treated seedlings: 1599 genes were significantly (P<0.05) induced, whereas 1133 genes were significantly repressed. Genes showing low expression ratios (>log2 1.0, <log2 −0.7) were removed, and the filtered dataset (291 induced and 129 repressed genes, respectively, Supplementary Dataset S3) was subjected to a Gene Ontology (GO) enrichment analysis (Ashburner et al., 2000). The ten GO biological process categories most overrepresented in the up-regulated gene set indicated that the peptide treatment induced processes related to biotic stress and responses to chemical substances (possibly derived from other organisms) (Fig. 9A). The GO categories most enriched among the down-regulated genes were related to cell wall modification and loosening (Fig. 9B).

Bottom Line: Here we present three novel members of the IDL subfamily and show that two of them are strongly and rapidly induced by different biotic and abiotic stresses.Expression patterns of the IDA/IDL and PIP/PIPL genes were analysed using in silico data, qRT-PCR and GUS promoter lines.Transcriptomic responses to PIPL3 peptide treatment suggested a role in regulation of biotic stress responses and cell wall modification.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.

No MeSH data available.


Related in: MedlinePlus